Polymorphism of coagulase gene in Staphylococcus aureus isolated from buffaloe milk
Subject Areas :
Food Science and Technology
جلال Shayegh
1
(
Assistant Professor, Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University (IAU), Shabestar, Iran.
)
A.R Monadi
2
(
Assistant Professor, Facultry of Veterinary Medicine, Tabriz Branch, Islamic Azad University (IAU), Tabriz, Iran.
)
Received: 2013-08-19
Accepted : 2013-12-17
Published : 2013-05-22
Keywords:
Milk,
polymorphism,
<i>Staphylococcous areues<i>,
Buffaloe,
Coagulase gen,
Abstract :
The aim of this study was to analyze the coA gene on Staphylococcus aureus isolates collected from milks of native buffaloes in north-west of Iran. For this purpose, seventy five S. aureus isolates were examined by PCR-RFLP. Amplification of coA gene revealed various amplicons with a sizes of approximately 600, 700, 760 and 850 bp in 12, 25, 29 and 9 of the isolates, respectively. AluI digestion of the amplicons demonstrated that the isolates had the same PCR band size in the same PCR-RFLP patterns. The result of coA gen analysis was similar to the isolates from cattle milk.
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