بهینهسازی پارامترهای موثر در استخراج اسانس از بذر (Heracleum persicum Desf. Ex Fischer) با استفاده از روش سطح پاسخ
محورهای موضوعی : گیاهان دارویی
1 - هیات علمی گروه باغبانی دانشگاه ارومیه
2 - گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه
کلید واژه: روش سطح پاسخ, استخراج اسانس روغن, بهینهسازی (Heracleum persicum Desf. Ex Fischer),
چکیده مقاله :
چکیده امروزه استفاده از اسانسهای گیاهی در صنایع غذا و دارو در حال افزایش است. لذا مطالعه حاضر، با هدف بهینهسازی فاکتورهای موثر در به حداکثر رساندن عملکرد اسانس و تعیین ترکیبات اسانس در شرایط بهینه صورت گرفت. اثر پارامترهای: مقادیر مختلف گیاه (X1)، زمان اسانسگیری (X2) و اندازه ذرات بذرها با مش (X3)، در سه سطح (1، 0، 1-) به‌عنوان متغیر مستقل، برای بهینهسازی درصد اسانس به عنوان متغیر وابسته با استفاده از روش سطح پاسخ بررسی شد. پس از جمع‌آوری بذرهای گیاه گلپر از منطقه آینالو (آذربایجان شرقی) در مرداد ماه 1394، اسانس به روش تقطیر با آب توسط دستگاه کلونجر استخراج شد و شناسایی ترکیبات اسانس با استفاده از دستگاه GC-MS صورت گرفت. نتایج تجزیه ANOVA و اشتباه آزمایشی برای مدل RSM نشان داد که مدل پیشبینی شده برای درصد اسانس به‌طور معنیداری دقیق است (P
Abstract In the recent years use of plant essential oils (EOs) in the food and medicine industry is increasing. So, the aim of this study was to optimize effective factors for maximizing essential oil yield and evaluation of its composition under the optimal conditions. The effect of different parameters including different amounts of plant (X1), extraction time of essential oils (X2) and particle size of seeds obtained with mesh (X3) on three levels (1, 0, 1-) as independent variable to optimize essential oil percentage as dependent variables were evaluated using response surface methodology. The seeds of Heracleum persicum Desf. Ex Fischer were collected from Ayalono area (East Azerbaijan) in August 2015. The essential oil was extracted by Clevenger apparatus and were identified by GC/MS. ANOVA analysis results and experimental error for the RSM model were showed that the predicted model for the essential oil percentage was significantly accurate (P
References
1.Amini, M., Younesi, H., Bahramifar, N., Lorestani, A.A.Z., Ghorbani, F., Daneshi, A. and Sharifzadeh, M. 2008. Application of response surface methodology for optimization of lead biosorption in an aqueous solution by Aspergillus niger. Journal of hazardous materials, 154(1-3):694-702.
2.Ara, K.M., Jowkarderis, M. and Raofie, F. 2015. Optimization of supercritical fluid extraction of essential oils and fatty acids from flixweed (Descurainia Sophia L.) seed using response surface methodology and central composite design. Journal of food science and technology, 52(7):4450-4458.
3.Bakkali, F., Averbeck, S., Averbeck, D. and Idaomar, M. 2008. Biological effects of essential oils–a review. Food and Chemical Toxicology, 46(2): 446-475.
4.DarvishiCheshmehSoltani, R., Rezaee, A., Khataee, A.R. and Godini, H. 2014. Optimisation of the operational parameters during a biological nitrification process using response surface methodology.The Canadian Journal of ChemicalEngineering,92(1): 13-22.
5.Davari, M. and Ezazi, R. 2017. Chemical composition and antifungal activity of the essential oil of Zhumeriamajdae, Heracleumpersicum and Eucalyptus sp. against some important phytopathogenic fungi.Journal de mycologiemedicale, 27(4):463-468.
6.Dehghan, H., Sarrafi, Y., Salehi, P. and Ebrahimi, S.N. 2017. α-Glucosidase inhibitory and antioxidant activity of furanocoumarins from Heracleumpersicum. Medicinal ChemistryResearch, 26(4): 849-855.
7.Dey, S.S. and Dora, K.C. 2014. Optimization of the production of shrimp waste protein hydrolysate using microbial proteases adopting response surface methodology.Journal of food science and technology, 51(1):16-24.
8.Ebrahimniaketai, SH. and Golestan, L. 2015. the factors affecting the extraction of essential oils using supercritical fluids, Twenty-third National Congress of Food Science and Technology, Quchan, Islamic Azad University, Quchan.
9.Ebadollahi, A., Zavieh, E.A., Nazifi, A., Sendi, J.J., Farjaminezhad, M., Samadzadeh, A. and Tajmiri, P. 2014. Chemical composition and bio-pesticidal values of essential oil isolated from the seed of HeracleumpersicumDesf. ex Fischer (Apiaceae). Spanish Journal of Agricultural Research, 12(4): 1166-1174.
10.Fattahi, M. and Fattahi, B. 2010. Principles of medicinal plants.First Edition.Publications of Tehran University Jihad-e Daneshgahi publication.473 p.
11.Ghahraman, A. 1996. The Scientific Names of 2000 Plants in Iranian Color Flora.First Edition.Forestry and Rangeland Research Institute, 347p.
12.Hajhashemi, V., Sajjadi, S.E. and Heshmati, M. 2009. Anti-inflammatory and analgesic properties of Heracleumpersicum essential oil and hydroalcoholic extract in animal models. Journal of Ethnopharmacology, 124(3): 475-480.
13.Hajhashemi, V., Dashti, G., Saberi, S. and Malekjamshidi, P. 2014. The effect of hydroalcoholic extract and essential oil of Heracleumpersicum on lipid profile in cholesterol-fed rabbits.AvicennaJournal of phytomedicine, 4(3): p.144.
14.Hemati, A., Azarnia, M. and Angaji, A.H. 2010. Medicinal effects of Heracleumpersicum(Golpar). Middle-East Journal of Scientific Research, 5(3): 174-176.
15.Kan, Y., Chen, T., Wu, Y. and Wu, J., 2015. Antioxidant activity of polysaccharideextracted from Ganodermalucidum using response surface methodology. International journal of biological macromolecules, 72: 151-157.
16.Khazaei, K.M., Jafari, S.M., Ghorbani, M., Kakhki, A.H. and Sarfarazi, M. 2016. Optimization of anthocyanin extraction from saffron petals with response surface methodology.Food Analytical Methods, 9(7): 1993-2001.
17.Larkeche, O., Zermane, A., Meniai, A.H., Crampon, C. and Badens, E. 2015. Supercritical extraction of essential oil from Juniperuscommunis L. needles: Application of response surface methodology. The Journal of Supercritical Fluids, 99: 8-14.
18.Majidi, Z. and Lamardi, S.N.S. 2018. Phytochemistry and biological activities of Heracleumpersicum: a review. Journal of integrative medicine, 3: 34-51.
19.Mozaffarian, V. 2007. Iran Flora Apiaceae family.Research Institute forests and rangelands, 580p.
20.Mojab, F., Rustaiyan, A.H. and Jasbi, A.R. 2002.Essential oils of HeracleumpersicumDesf.ex Fischer leaves. DARU Journal of Pharmaceutical Sciences, 10(1): 6-10.
21.Noorimotlagh, Z., Shahriyar, S., DarvishiCheshmehSoltani, R. and Tajik, R. 2016. Optimized adsorption of 4-chlorophenol onto activated carbon derived from milk vetch utilizing response surface methodology. Desalination and Water Treatment, 57(30): 14213-14226.
22.Puértolas, E., Saldaña, G., Álvarez, I. and Raso, J. 2011. Experimental design approach for the evaluation of anthocyanin content of rosé wines obtained by pulsed electric fields. Influence of temperature and time of maceration. Food Chemistry, 126(3): 1482-1487.
23.Radjabian, T., Salimi, A., Rahmani, N., Shockravi, A. and Mozaffarian, V. 2013.Essential oil composition of some wild populations of HeracleumpersicumDesf.Ex Fischer growing in Iran. Journal of Essential Oil Bearing Plants, 16(6): 841-849.
24.Raut, J.S. and Karuppayil, S.M. 2014. A status review on the medicinal properties of essential oils.Industrial Crops and Products, 62: 250-264.
25.Saeidnia, S., Gohari, A.R., Hadjiakhoondi, A., Afrapoli, F.M.and Shafiee, A. 2005. Cytotoxicity and chemical constituents of the volatile oil of golpar (HeracleumpersicumDesf.Ex Fischer). Bioscience Research, 2:107-10.
26.Sayyah, M., Moaied, S. and Kamalinejad, M. 2005. Anticonvulsant activity of Heracleumpersicum seed. Journal of Ethno pharmacology, 98(1): 209-211.
27.Sodeifian, G., Ardestani, N.S., Sajadian, S.A. and Ghorbandoost, S. 2016. Application of supercritical carbon dioxide to extract essential oil from Cleome coluteoidesBoiss.: experimental, response surface and grey wolf optimization methodology. The Journal of Supercritical Fluids, 114: 55-63.
28.Srivastava, M., Maheshwari, S., Kundra, T.K. and Rathee, S. 2017. Multi-response optimization of fused deposition modelling process parameters of ABS using response surface methodology (RSM)-based desirability analysis. Materials Today: Proceedings, 4(2):1972-1977.
29.Walasek, M., Grzegorczyk, A., Malm, A. and Skalicka-Woźniak, K. 2015. Bioactivity-guided isolation of antimicrobial coumarins from HeracleummantegazzianumSommier& Levier (Apiaceae) fruits by high-performance counter-current chromatography. Food chemistry, 186:133-138.
30.Walia, A., Mehta, P., Guleria, S. and Shirkot, C.K. 2015. Improvement for enhanced xylanase production by Cellulosimicrobiumcellulans CKMX1 using central composite design of response surface methodology.3 Biotech, 5(6): 1053-1066.
31.Xu, H., Xu, X., Tao, Y., Yuan, F. and Gao, Y. 2015. Optimization by response surface methodology of supercritical carbon dioxide extraction of flavour compounds from Chinese liquor vinasse. Flavour and fragrance journal, 30(4): 275-281.
32.Yılmaz, F.M., Karaaslan, M. and Vardin, H. 2015. Optimization of extraction parameters on the isolation of phenolic compounds from sour cherry (Prunuscerasus L.) pomace. Journal of Food Science and Technology, 52(5): 2851-2859.
_||_