Optimization of Effective Parameters in Essential Oil Extraction of Heracleum persicum Desf. Ex Fischer Seed Using Response Surface Methodology
Subject Areas : Medicinal Plantsمحمد فتاحی 1 , maryam rezapoor 2
1 - Department of Horticulture- Faculty of Agriculture- Urmia University
2 - departmet of Horticuture, faculty of agriculture, urmia university
Keywords: Optimization, response surface methodology, essential oil extraction, Heracleum persicum Desf. Ex Fischer,
Abstract :
Abstract In the recent years use of plant essential oils (EOs) in the food and medicine industry is increasing. So, the aim of this study was to optimize effective factors for maximizing essential oil yield and evaluation of its composition under the optimal conditions. The effect of different parameters including different amounts of plant (X1), extraction time of essential oils (X2) and particle size of seeds obtained with mesh (X3) on three levels (1, 0, 1-) as independent variable to optimize essential oil percentage as dependent variables were evaluated using response surface methodology. The seeds of Heracleum persicum Desf. Ex Fischer were collected from Ayalono area (East Azerbaijan) in August 2015. The essential oil was extracted by Clevenger apparatus and were identified by GC/MS. ANOVA analysis results and experimental error for the RSM model were showed that the predicted model for the essential oil percentage was significantly accurate (P
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