بررسی فیتوشیمیایی و آنتی اکسیدانی ترکیبات موثره و فعالیت آنتی اکسیدانی اسانس اکوتیپ های مختلف گیاه دارویی ( .Oliveria decumbens Vent) در استان خوزستان
محورهای موضوعی : گیاهان داروییسید محمد حسین آل عمرانی نژاد 1 , علی رضوانی اقدم 2
1 - گروه کشاورزی، دانشگاه آزاد اسلامی، واحد بین المللی خرمشهر-خلیج فارس، خرمشهر، ایران.
2 - گروه کشاورزی، دانشگاه آزاد اسلامی، واحد بین المللی خرمشهر- خلیج فارس، خرمشهر، ایران.
کلید واژه: اسانس, خوزستان, آنتی اکسیدان, لعل کوهستان,
چکیده مقاله :
گیاهان معطر بعنوان با ارزش ترین منبع آنتی اکسیدانی در طبیعت محسوب می شوند. در این تحقیق اکوتیپ های مختلف گیاه لعل کوهستان (Oliveria decumbens Vent.) در مرحله گلدهی کامل، از سه رویشگاه مهم در استان خوزستان شامل بهبهان، شوشتر و مسجد سلیمان جمع آوری گردید. اسانس گیاهان جمع آوری شده، به روش تقطیر با آب توسط دستگاه کلونجر استخراج گردید و ترکیبات آن توسط دستگاه گازکروماتوگرافی با طیف سنج جرمی(GC-Mass)، شناسایی شد. نتایج حاصل نشاندهنده غالب بودن تیمول و کارواکرول بود، که بیشترین مقدار آنها به ترتیب با 67/29 و 88/27 درصد از گیاهان برداشت شده از منطقه بهبهان حاصل شد. درصد تخریب رادیکالهای آزاد DPPH در غلظت های مختلف اسانس شامل 50، 100، 200، 300 و 400 میکرولیتر(µl) جهت تعیین فعالیت آنتی اکسیدانی اسانس، محاسبه شد. تست آنتی اکسیدانی، لعل کوهستان را از نظر خواص آنتی اکسیدانی، گیاهی پر اهمیت نشان داد. در این تحقیق میزان IC50 لعل کوهستان بومی منطقه شوشتر، مسجد سلیمان و بهبهان به ترتیب21/34 ،85/26 و 23/21 بدست آمد. درنهایت می توان گفت اکوتیپ بهبهان با بیشترین خاصیت آنتی رادیکالی می تواند بعنوان منبع طبیعی جدیدی جهت تامین آنتی اکسیدان، در نظر گرفته شود.
Aromatic herbs are considered the most valuable antioxidant source in nature. In this research, 3 different ecotypes of Oliveria decumbens Vent plant were collected from three important regions in Khuzestan province, including Behbahan, Shushtar and Masjed Soleyman in flowering stage. Essential oil was extracted by Clevenger aparatus; and identified essential oil component by gas chromatography/mass spectrometry(GC -Mass). The antioxidant activities were specified using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The highest Thymol (29.67) and Carvacrol (27.88%), the dominant compositions in essential oil, were detected in Behbahan region. The percentage of degradation of DPPH free radicals in different concentrations of essential oil including 50, 100, 200, 300, and 400 μl were calculated to determine the antioxidant activity of the essential oil. The IC50 values were calculated 34.21, 26.85 and 21.23 respectively for harvested plants from Shushtar, Masjed Soleyman and Behbahan regions. Finally, Behbahan ecotype with the highest antiradical properties can be considered as a new natural source of antioxidant supply.
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