بررسی پروفایل اسید آمینه پروتئین هیدرولیز شده بدست آمده از آنابنا و خواص ضداکسیدانی و ضدباکتریایی آن
الموضوعات :مهدیه دراج 1 , مهناز سادات صادقی 2 , مژگان امتیازجو 3 , ندا سلطانی 4 , فریبا زمانی هرگلانی 5
1 - دانشجوی دکتری، دانشکده علوم و فنون دریایی، گروه زیست شناسی دریا، دانشگاه آزاداسلامی، واحد تهران شمال. تهران، ایران
2 - استادیار دانشکده علوم و فنون دریایی، گروه زیست شناسی دریا، دانشگاه آزاداسلامی، واحد تهران شمال. تهران، ایران.
3 - گروه بیولوژی دریا، دانشکده علوم و فون دریایی دانشگاه آزاد اسلامی واحد تهران شمال
4 - گروه پژوهشی میکروبیولوژی نفت، پژوهشکده علوم پایه کاربردی جهاددانشگاهی
5 - دانشگاه آزاد اسلامی واحد علوم و تحقیقات
الکلمات المفتاحية: آلکالاز, پروتئین هیدرولیز شده, خواص ضداکسیدانی, خواص ضدباکتریایی, آنابنا,
ملخص المقالة :
چکیده مقدمه: مقدمه: امروزه سیانوباکتری‌ها که گروهی از جلبک‌های میکروسکوپی هستند که بعنوان منبع پروتئینی برای تولید پروتئین هیدرولیز شده مورد توجه قرار گرفته¬اند. مواد و روش کار: پروتئین استخراج شده از آنابنا توسط آنزیم آلکالاز در دمای 55 درجه سانتی گراد و pH 8 به مدت 8 ساعت هیدرولیز شد. پروفایل اسید آمینه پروتئین هیدرولیز شده توسط یک سیستم RP-HPLC مورد بررسی قرار گرفت. خواص ضداکسیدانی نمونه¬های بدست آمده با روش سرکوب کنندگی رادیکال¬های آزاد DPPH، ABTS و شلاته کنندگی یونهای فلزی سنجیده شد. در ادامه خواص ضدباکتریایی پروتئین هیدرولیز شده تولید شده در برابر تعدادی از پاتوژنهای بیماری¬زا بررسی شد. نتایج و بحث: پروفایل اسید آمینه پروتئین هیدرولیز شده بدست آمده نشان داد که این نمونه حاوی مقادیر بالاتری نسبت به نیاز روزانه آمینواسیدی کودکان و بزرگسالان بود و همچنین مقادیر مناسبی از اسیدهای آمینه ضروری را نیز داشت. بالاترین درصد خنثی کنندگی در برابر رادیکال های آزاد DPPH و ABTS به تریبت 22/88% و 90/68% بود. بالاترین فعالیت شلاته کنندگی نمونه بدست آمده 69/62% در غلظت 2 میلی¬ گرم/میلی لیتر مشاهده شد. همچنین نتایج نشان داد بالاترین خواص ضدباکتریایی نمونه مورد بررسی در برابر باکتریهای استافیلوکوکوس اورئوس، لیستریا مونوسیتوژنز، اشریشیا کلی و سالمونلا تیفی موریوم به ترتیب 02/6، 90/5، 30/6 و 80/3 میلی متر در غلظت 100 میلی¬گرم / میلی¬لیتر بود (P< 0.05). نتیجه گیری: بر اساس نتایج بدست آمده مشخص گردید که پروتئین استخراج شده از آنابنا قابلیت استفاده برای تولید پروتئین هیدرولیز شده با ویژگی های ضداکسیدانی، ضدباکتریایی را دارد و پتانسیل استفاده در صنایع غذایی و دارویی را دارد.
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