This work aimed to make a smart biofilm based on tapioca starch/bovine gelatin and roselle calyx extract (RCE)/nanofiller-ZnO. Then the utilization of the biofilm was studied to monitor the rainbow trout fillets. The biofilms were fabricated by casting the starch/gelatin solution with sorbitol /glycerol (3.2g) on casting plates and were dried at 25C. RCE was added into biofilms (0.5 g for TP/RCE/ZnO-N and 2g for TP/RCE), and the biofilm characteristics, such as thickness, color, and antioxidant properties were investigated. The films containing nanofiller-ZnO/RCE and RCE showed significantly (p0.05) more antioxidant activity than the neat film which respective values of 18.31 11.42 and 3.1. The thickness of the smart films significantly increased (p0.05) from 0.08 to 0.13 by the addition of RCE/nanofiller ZnO into the solution. According to the results, a value was significantly higher (p0.05) in smart film with nanofiller-ZnO/RCE and RCE. The lowest L* value was obtained in film with nanofiller-ZnO/RCE. At the end of the conservation, the TVB-N value reached 35.7 mg N/100 and with the TVB-N significantly increase (p0.05), the color properties of the smart films also changed. The color of the biofilm was purple, but the a value significantly decreased. Therefore, the film based tapioca starch/bovine gelatin/nanofiller ZnO/RCE can be applied as a natural indicator of rainbow trout fillets.
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