Evaluating the Antimicrobial Effect of Eucalyptus globulus Oil on Yeast Kluyveromyces marxianus: A Study on Biopreservative in Heat Treated Noncarbonated Doogh
Subject Areas : food microbiologyN. Rezaee 1 , A. S. Tabatabaee Bafroee 2 , A. Sharifan 3
1 - M. Sc. Graduated of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Assistant Professor of the Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
3 - Associate Professor of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Keywords: Doogh, Eucalyptus Essential Oil, Kluyveromyces marxianus, Natural Preservative, Sensory Characteristics,
Abstract :
The purpose of this study was to investigate the application of natural preservatives as an alternative to chemical preservatives in the production of Doogh. In order to carry out the work, different concentrations (0.25, 0.5, 0.75, 1, 1.5, 3%) of Eucalyptus essential oil were employed to study the growth of Kluyveromyces marxianus in Doogh at 4 °C and 15°C within 9 days. The essential oil of the plant was prepared by the hydro-distillation method and the chemical analysis was carried out by the application of Gas Chromatography -Mass Spectroscopy (GC/MS). Minimum Inhibitory Concentration (MIC) for Eucalyptus oil was determined through an agar diffusion method. The inhibitory effect and sensory characteristics of the oil were measured during 9 days of storage at 4 and 25°C. Chemical analysis of the oil indicated the presence of 15 compounds where Eucalyptol was the major constituent (78.68%) followed by γ - Terpinene (7.02%), α- Pinene (4.16%) and α- Terpinene (1.69%). The results showed that the number of K. marxianus in Doogh during storage time depended on the concentration of the used essential oil in the samples and storage temperature. It therefore might be concluded that the application of Eucalyptus essential oil to samples stored at 4°C and 25°C as a natural preservative might improve the desirable microbial, chemical and sensory properties of Doogh.
Anon. (2008a). Doogh Specifications and test method. National Standard No. 2453. URL: http://www.isiri.org/portal/files/ std/2453. pdf. Accessed 20 May 2015.
Ah Lee, J. & Chee, H. Y. (2010) In Vitro Antifungal Activity of Equol against Candida albicans. Mycobiology, 38(4), 328-330.
Anon. (2016). Performance standards for antimicrobial susceptibility testing; Twenty-sixth informational supplement. Clinical and Laboratory Standards Institute (CLSI) document M100-S26. Wayne, PA: Clinical and Laboratory Standards Institute.
Bachir, R. & Benali, M. (2012). Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pacific Journal of Tropical Biomedical, 2(9), 739-742.
Basma, A., Asrar, A. & Kurji, M. (2013). Extraction of Oil from Eucalyptus camadulensis Using Water Distillation Method. Iraqi Journal of Chemical and Petroleum Engineering, 14(2), 7- 12.
Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods. International Journal of Food Microbiology, 94(3), 223-253.
Buttriss, J. (1997). Nutritional properties of fermented milk products. International Journal of Dairy Technology, 50(1), 21-27.
Fathi, E., Sefidkon, F. & Abravesh, Z. (2009). The Effects of Drying and Extraction Methods on Essential Oil Content and Composition of Eucalyptus spathulata. Jeobp, 12 (2), 189 – 197.
Gilles, M., Zhao, J., An, M. & Agboola, S. (2010) Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species. Food Chemistry, 119, 731–737.
Horie, K., Horie, N., Abdou, A. M., Yang, J. O., Yun, S. S., Chun, H. N., Park, C. K., Kim, M. & Hatta, H. (2004). Suppressive effect of functional drinking yogurt containing specific egg yolk immunoglobulatin on Helicobacter pylori in humans. American dairy science association, 87(12), 4073-4079.
Maciel, M. V., Morais, S. M., Bevilaqua, C. M. L., Silva, R. A., Barros, R. S., Sousa, R. N. & Souza-Neto, M. A. (2010). Chemical composition of Eucalyptus spp. essential oils and their insecticidal effects on Lutzomyia longipalpis. Veterinary Parasitology, 167(1), 1-7.
Melani, M. L. & Morrissey, J. P. (2010). Kluyveromyces marxianus: A yeast emerging from its sister, s shadow. Fungal Biology Reviews, 24, 17-26.
Najafian, M., Karimi, H. & Zand, N. (2015). Antimicrobial Effect of Spearmint and Dill oils on Yeast Kluyveromyces marxianus (K. marxianus) in Iranian Doogh. Biological Forum – An International Journal, 7(2), 69-74.
Nikooyeh, B., Neyestani, T., Tayebinejad, N. & Alavimajd, H. (2014) Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D. Journal of Human Nutrition and Dietetics, 27 (2), 276-283.
Nasab asgarian, H., Sajadi, E., Sadeghi, H., Naderian, M. & Abedi, A. (2012). The survey of phytochemical of Ferulago an gulate (schlecht.) Boiss. National congress of medicine herbal 5-8 September, yasooj.
Ogunwande, I. A., Olawore, N. O., Adeleke, K. A., Ekundayo, O. & Koenig, W. A. (2003). Chemical composition of the leaf volatile oil of Psidium guajava L. growing in Nigeria. Flavour and Fragrance Journal, 18(2), 136-138.
Oussalah, M., Caillet, S., Saucier, S. & Lacroix, M. (2005). Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control, 18, 414-420.
Singh, G., Maurya, S. & Catalan, C. (2005). Chemical constituent's antimicrobial investigations and antioxidant potentials of Anethum graveolens essential oil and acetone extract, Journal of Food Science, 70, 15- 208.
Soltani, M., Say, D. & Güzeler, N. (2012) Production and Quality Characteristics of “Doogh”. Akademik Gıda® / Academic Food Journal, 10(4), 50-53.
Tamime, A. Y. & Robinson, R. K. (2001). Yoghurt, science and technology, 2 ED, CRC press, England, 249305, 535-587.
Teixeira, B., Marques, A., Ramos, C., Neng, N. R., Nogueira, J. M., Saraiva, J. A. & Nunes, M. L. (2013). Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Industrial Crops and Products, 43, 587-595.
Viljoen, B. C. (2003). Temperature abuse initiating yeasts growth in yoghurt. Food Research International, 36, 193-197.