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مقاله
1 - Investigation of Bovine Leukocyte Adhesion Deficiency (BLAD) and Complex Vertebral Malformation (CVM) in a Population of Iranian Holstein CowsIranian Journal of Applied Animal Science , شماره 1 , سال 5 , زمستان 2015In the present research, molecular detection of bovine leukocyte adhesion deficiency (BLAD) and complex vertebral malformation (CVM)in a population of Iranian Holstein cows has been carried outusing milk somatic cells by polymerase chain reaction-restriction fragment le چکیده کاملIn the present research, molecular detection of bovine leukocyte adhesion deficiency (BLAD) and complex vertebral malformation (CVM)in a population of Iranian Holstein cows has been carried outusing milk somatic cells by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The BLAD and CVM are monogenic and autosomal recessive heredity lethal syndrome in Holstein-Friesian cattle. BLAD characterized by affecting the haematopoietic system via reduced expression of the adhesion molecules on neutrophils. CVM characterized by intra-uterine mortality with disorders such as short neck, curved legs, abnormality of ribs and some certain heart abnormalities. In the first step of our research program,tank milk samples from 50 herds were collected. PCR-RFLP was performed to detect a point mutation of both CVM and BLAD genes. After DNA extraction, PCR was amplified using specific primers for 136 bp DNA (CD18 gene) and233 bp DNA (SLC35A3 gene).TaqIand EcoT22I enzymes were used to identify both BLAD and CVM alleles of both genes by digestion of PCR products.In these herds, we did not find any affected herd with the mutant allele of BLAD comparing with a positive evidence but the mutation of SLC35A3 gene found in 17 different herds. In the next step of our study a herd with 120 cows was randomly selected for individual test using blood samples. We showed two cows out of 120 were identified as carriers of this gene. In this herd, the total number of dominant homozygote (AA), heterozygote (Aa) and recessive homozygote (aa) genotypes for CVM were 118, 2 and 0, respectively and the frequency of A and a alleles were 0.992 and 0.008, respectively. The other affected herds will be tested in the next step of our research program. پرونده مقاله -
مقاله
2 - Association between Insulin–Like Growth Factor I Polymorphism and Early Growth Traits in Iranian Zandi Sheep, Found Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP)Iranian Journal of Applied Animal Science , شماره 4 , سال 6 , تابستان 2016Early growth traits in sheep are economically important. Breeding for these traits has been shown to be problematic using quantitative genetic methods, particularly in native sheep herds. Molecular genetics is useful for sheep breeding. The gene for insulin-like growth چکیده کاملEarly growth traits in sheep are economically important. Breeding for these traits has been shown to be problematic using quantitative genetic methods, particularly in native sheep herds. Molecular genetics is useful for sheep breeding. The gene for insulin-like growth factor 1 (IGF1) is one of the important candidate genes known for ovine early growth traits. The aim of this study was to investigate IGF1 polymorphism in exon 1 and its association with some early growth traits in Iranian Zandi sheep. For this purpose, 120 male and female lambs were selected randomly at up to 5 months old and blood samples were taken individually. Also, phenotypic records were collected from related farms. Genomic DNA was extracted by a salting-out method. A 265 bp region in exon 1 of IGF1 was amplified by polymerase chain reaction (PCR), then genotyped using the restriction endonuclease HaeII. Two alleles, here called A and B, were found. Examination using PopGene32 showed allele frequencies of 0.47 A and 0.53 B. Genotype % frequencies were 28.33 AA, 37.50 AB and 34.17 BB. Chi-squared and G-squared tests showed that the population was not at Hardy-Weinberg equilibrium (P<0.01). An association study in SAS 9.2 found no significant effect of IGF1 genotype on any of these early growth traits: birth weight, average daily gain up to age five months and weight at age five months. However the AA genotype was linked to the highest values of birth weight. IGF1 polymorphism should be investigated as a molecular marker along with other polygene effects for growth traits in native sheep breeding programs. پرونده مقاله -
مقاله
3 - اثر سه نوع افزودنی خوراکی کوآنزیم10 Q ،ال کارنی تین و راکتوپامین بر عملکردوبرخی فراسنجه های خونی در جوجه های گوشتیدانش و پژوهش علوم دامی , شماره 5 , سال 93 , زمستان 1393 -
مقاله
4 - برآورد مؤلفههای واریانس و کواریانس صفت رشد با استفاده ازمدل تابعیت تصادفی در گوسفند زندیدانش و پژوهش علوم دامی , شماره 5 , سال 91 , زمستان 1391 -
مقاله
5 - بررسی اثر ورم پستان تحت بالینی بر لاکتوز، مواد جامد و pH شیر در بز سانندانش و پژوهش علوم دامی , شماره 5 , سال 93 , زمستان 1393 -
مقاله
6 - بررسی نقص چسبندگی گلبولهای سفید خون در جمعیتی از گاوهای هلشتاین ایرانیدانش و پژوهش علوم دامی , شماره 2 , سال 92 , تابستان 1392