The goal of this study was investigation of the calpastatin gene polymorphism and its effect on early body weight traits in Zandi sheep by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. Calpastatin is the main inhibitors of calpain-calpastatin enzyme system that play an important role in regulating the protein regeneration process. Genomic DNA was extracted by whole blood samples from 124 randomly selected Zandi breed sheep using salting out method. Birth weight, weight on 150 days old and daily weight gain from birth to 150 days old were recorded. The exon and intron 1 of ovine calpastatin gene were amplified to produce a 622 bp fragment. The PCR products were digested with MspI restriction enzyme and electrophoresised on agarose gel. Results showed 80.6% and 19.4% allele frequencies for A and B alleles and 65.32%, 4.04% and 30.64% genotype frequencies for AA, BB and AB genotypes, respectively. X2 and G2 tests showed the Hardy-Weinberg equilibrium for studied locus. The effect of calpastatin genotype on growth traits was insignificant, but genotype BB had the best performance among all the three traits, which indicates the better performance of normal allele (B) than mutant allele (A) for these traits. پرونده مقاله

Early growth traits in sheep are economically important. Breeding for these traits has been shown to be problematic using quantitative genetic methods, particularly in native sheep herds. Molecular genetics is useful for sheep breeding. The gene for insulin-like growth factor 1 (IGF1) is one of the important candidate genes known for ovine early growth traits. The aim of this study was to investigate IGF1 polymorphism in exon 1 and its association with some early growth traits in Iranian Zandi sheep. For this purpose, 120 male and female lambs were selected randomly at up to 5 months old and blood samples were taken individually. Also, phenotypic records were collected from related farms. Genomic DNA was extracted by a salting-out method. A 265 bp region in exon 1 of IGF1 was amplified by polymerase chain reaction (PCR), then genotyped using the restriction endonuclease HaeII. Two alleles, here called A and B, were found. Examination using PopGene32 showed allele frequencies of 0.47 A and 0.53 B. Genotype % frequencies were 28.33 AA, 37.50 AB and 34.17 BB. Chi-squared and G-squared tests showed that the population was not at Hardy-Weinberg equilibrium (P<0.01). An association study in SAS 9.2 found no significant effect of IGF1 genotype on any of these early growth traits: birth weight, average daily gain up to age five months and weight at age five months. However the AA genotype was linked to the highest values of birth weight. IGF1 polymorphism should be investigated as a molecular marker along with other polygene effects for growth traits in native sheep breeding programs. پرونده مقاله

تحقیق حاضر با هدف بررسی اثر اسید آسکوربیک و هیدرو کسی آنالوگ متیونین بر عملکرد و بافت‌شناسی کبد جوجه‌های گوشتی تحت شرایط تنش گرمایی انجام شد. جهت انجام ازمایش از 400 قطعه جوجه نر یکروزه سویه تجاری راس 308 در قالب یک طرح کاملا تصادفی با 4 تیمار و 5 تکرار (هر تکرار حاوی 20 قطعه جوجه) استفاده شد. تیمار های آزمایشی عبارت بودند از: 1) جیره پایه بر اساس ذرت-سویا (شاهد)، 2) جیره پایه به همراه 2/0 درصد ویتامین C، 3) جیره پایه به همراه 46/0، 36/0 و 32/0 درصد متیونین هیدروکسی آنالوگ به ترتیب در دوره اغازین، رشد و پایانی و 4) جیره پایه به همراه مخلوط 2/0 درصد ویتامین C و 46/0، 36/0 و 32/0 درصد متیونین هیدروکسی آنالوگ به ترتیب در دوره اغازین، رشد و پایانی. در دوره رشد خوراک مصرفی در تیمار حاوی متیونین هیدروکسی آنالوگ افزایش معنی داری با شاهد داشت (05/0 > p). در دوره پایانی ضریب تبدیل خوراک در تیمارهایی که از مخلوط ویتامین C و متیونین هیدروکسی آنالوگ استفاده کرده بودند بهبود معنی داری با شاهد داشتند (05/0 >p ). قطر هپاتوسیت و قطر هسته هپاتوسیت در جوجه هایی که از ویتامین C و مخلوط ویتامین C و متیونین هیدروکسی آنالوگ استفاده کرده بودند کاهش معنی داری با شاهد داشتند (05/0>P). نتایج این آزمایش نشان داد که بکار بردن مخلوط ویتامین C و متیونین هیدروکسی آنالوگ نسبت به استفاده جداگانه از آن ها در جیره غذایی می تواند بر کاهش آثار منفی تنش حرارتی موثر باشد. پرونده مقاله