Association between Insulin–Like Growth Factor I Polymorphism and Early Growth Traits in Iranian Zandi Sheep, Found Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP)
محورهای موضوعی : Camelف. نظری 1 , ع. نوشری 2 , ب. همتی 3
1 - Department of Animal Science, Faculty of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran
2 - Department of Animal Science, Faculty of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran
3 - Department of Animal Science, Faculty of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran
کلید واژه: Zandi sheep, early growth traits, allele frequency, genotype frequency, <i>IGF1</i>,
چکیده مقاله :
Early growth traits in sheep are economically important. Breeding for these traits has been shown to be problematic using quantitative genetic methods, particularly in native sheep herds. Molecular genetics is useful for sheep breeding. The gene for insulin-like growth factor 1 (IGF1) is one of the important candidate genes known for ovine early growth traits. The aim of this study was to investigate IGF1 polymorphism in exon 1 and its association with some early growth traits in Iranian Zandi sheep. For this purpose, 120 male and female lambs were selected randomly at up to 5 months old and blood samples were taken individually. Also, phenotypic records were collected from related farms. Genomic DNA was extracted by a salting-out method. A 265 bp region in exon 1 of IGF1 was amplified by polymerase chain reaction (PCR), then genotyped using the restriction endonuclease HaeII. Two alleles, here called A and B, were found. Examination using PopGene32 showed allele frequencies of 0.47 A and 0.53 B. Genotype % frequencies were 28.33 AA, 37.50 AB and 34.17 BB. Chi-squared and G-squared tests showed that the population was not at Hardy-Weinberg equilibrium (P<0.01). An association study in SAS 9.2 found no significant effect of IGF1 genotype on any of these early growth traits: birth weight, average daily gain up to age five months and weight at age five months. However the AA genotype was linked to the highest values of birth weight. IGF1 polymorphism should be investigated as a molecular marker along with other polygene effects for growth traits in native sheep breeding programs.
صفات اولیه رشد در گوسفند از نظر اقتصادی دارای اهمیت هستند. اصلاح نژاد برای این صفات با استفاده از روشهای ژنتیک کمی به ویژه در گلههای بومی گوسفند با مشکلات زیادی مواجه است. ژنتیک مولکولی یکی از ابزارهای کارآمد در اصلاح نژاد گوسفند محسوب میشود. ژن فاکتور 1 رشد شبه انسولین (IGF1) یکی از ژنهای کاندیدای مهم برای صفات اولیه رشد در گوسفند شناخته شده است. هدف این تحقیق بررسی چندشکلی در اگزون شماره 1 این ژن و رابطه این چندشکلیها با عملکرد صفات اولیه رشد در گوسفندان زندی کشور ایران میباشد. برای این منظور تعداد 120 رأس از برههای نر و ماده در سن تا 5 ماهگی به طور تصادفی انتخاب شدند و خونگیری به صورت انفرادی از آنها انجام شد. همچنین رکوردهای فنوتیپی از دامداریهای مربوطه جمعآوری گردید. استخراج DNA به روش نمکی انجام شد. واکنش زنجیرهای پلیمراز (PCR) برای تکثیر ناحیه 265 bp اگزون 1 ژن IGF1 انجام شد و سپس با استفاده از آنزیم برشی HaeII تعیین ژنوتیپ انجام گردید. نتایج این تحقیق نشان دهنده وجود دو آلل شامل A وB بود. بررسی با استفاده از نرم افزار PopGene32 فراوانی آللهای A و B را به ترتیب 47/0 و 53/0 نشان داد. فراوانیهای ژنوتیپی برای سه ژنوتیپ AA، AB و BB به ترتیب 33/28، 50/37 و 17/34 بود. همچنین آزمونهای کای مربع و جی مربع نشان داد جمعیت در تعادل هاردی واینبرگ قرار ندارد (01/0>P). مطالعه ارتباط با استفاده از نرم افزار SAS 9.2 نشان داد اثر ژنوتیپهای IGF1 روی هیچ یک از صفات اولیه رشد شامل وزن تولد، وزن 5 ماهگی و میانگین افرایش وزن روزانه تولد تا 5 ماهگی معنیدار نیست. با این حال ژنوتیپ AA با عملکرد بیشتر برای وزن تولد دارای ارتباط بود. چندشکلیهای ژن IGF1 باید به عنوان یک نشانگر مولکولی در کنار اثر سایر پلی ژنهای مربوط به صفات رشد در برنامههای اصلاح نژاد در گوسفندان بومی مورد بررسی قرار گیرد.
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