Optimized Purification of Free Amino Acids from Molasses by Nanofiltration Membrane

محورهای موضوعی : food science

M. Varaee ¹ (Ph. D. Graduated of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
M. Honarvar ² (Associate Professor of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
M. H. Eikani ³ (Professor of the Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran.)
M. R. Omidkhah ⁴ (Professor of the Department of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.)
N. Mooraki ⁵ (Associate Professor of the Department of Fisheries Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.)

تاریخ دریافت : 1400/06/06 تاریخ پذیرش : 1400/09/22 تاریخ انتشار : 1401/10/11

کلید واژه: Alanine, Glutamic acid, Aspartic acid, Dead-end filtration, Lysine,

چکیده مقاله :

In this research, nanofiltration was utilized to purify free amino acids (AAs) from sugar and colloids of sugar cane molasses (SCM) and sugar beet molasses (SBM) based on their molecular weight. The impact of temperature (30-50˚C), pressure (2-7 bar), and pH (2-11) in optimizing the purification condition was evaluated using the response surface methodology. The SCM and SBM purification results revealed the same optimum conditions in both types of molasses: 47 ˚C temperature, 3 bar pressure, and 9.3-9.5 pH. In the optimum condition, the recovery values of AAs for SCM and SBM were 66% and 63% for aspartic acid, 68.5% and 66% for glutamic acid, 84% and 69% for alanine, and 82% and 69% for lysine, respectively. The total efficiency values of four AAs and flux were obtained as 75% and 70 Lm-2h-2 for SCM and 67% and 45 Lm-2h-2 for SBM, respectively. The results indicated the suitability of the nanofiltration system's purifying function for AAs from SCM and SBM with desirable selectivity and purification efficiency.

منابع و مأخذ:

Abdelmoez, W., Nakahasi, T. & Yoshida, H. (2007). Amino Acid Transformation and Decomposition in Saturated Subcritical Water Conditions. Industrial & Engineering Chemistry Research, 46(16), 5286–5294.

Abejón, R., Belleville, M.P., Sanchez-Marcano, J., Garea, A. & Irabien, A. (2017). Optimal Design of Industrial Scale Continuous Process For Fractionation By Membrane Technologies Of Protein Hydrolysate Derived From Fish Wastes. Separation and Purification Technology, 197, 137-146.

Alexander, S. P. H. (2009). Encyclopedia of Neuroscience. eds., Academic press, Cambridge Massachusetts. pp. 885–894.

Bernal, M. Ruiz, M.O., Geanta, R.M., Benito, J.M. & Escudero, I. (2016). Colour removal from beet molasses by ultrafiltration with activated charcoal. Chemical Engineering Journal, 283, 313-322.

Betancur-Ancona, D., Dávila-Ortiz, G., Chel-Guerrero, L.A. & Torruco-Uco. J.G. (2015). ACE-I inhibitory activity from Phaseolus lunatus and Phaseolus vulgaris peptide fractions obtained by ultrafiltration. Journal of Medicinal Food, 18, 1247-1254.

Bhagavan, N.V. & Ha, C.E. (2011). Essentials of Medical Biochemistry: With Clinical Cases. eds. pp. 19–27.

Bowen, W.R. & Mukhtar, H. (1996). Characterization and prediction of separation of nanofiltration membranes. Journal of Membrane Science, 112, 263–274.

Centenaro, G.S., Salas-Mellado, M., Pires, C., Batista, I., Nunes, M.L. & Prentice, C. (2014). Fractionation of protein hydrolysates of fish and chicken using membrane ultrafiltration: investigation of antioxidant activity. Applied Biochemistry and Biotechnology, 172, 2877-2893.

Chabeaud, A., Vandanjon, L., Bourseau, P., Jaouen, P., Chaplain-Derouiniot, M. & Guerard, F. (2009). Performances of ultrafiltration membranes for fractionating a fish protein hydrolysate: Application to the refining of bioactive peptidic fractions. Separation and Purification Technology, 66, 463-471.

Chakrabarty, T., Thakur, A.K. & Shahi, V.K. (2013). Functionalized chitosan based nano-filter membranes for pH-controlled separation of amino acids. Separation and Purification Technology, 108, 57–64.

Clarke, M.A. (2003). Syrups, In: Encyclopedia of Food Sciences and Nutrition (Second Edition). pp. 5711-5717.

Clarke, M.A. & Edye, L.A. (1996). Sugar beet and sugar cane as renewable resources, In: Fuller, G., McKeon, T.A., Bills, D.D. (Eds.), Agricultural materials as renewable resources, American Chemical Society. pp. 231-237.

Coca, M., Garcia, M.T., Gonzalez, G., Pena, M. & Garcia, J.A. (2004). Study of coloured components formed in sugar beet processing. Food Chemistry, 86, 421–433.

Córdovaa, A., Astudilloa, C., Santibanez, L., Cassano, A., Ruby-Figueroad, R. & Illanes, A. (2017). Purification of galacto-oligosaccharides (GOS) bythree-stage serial nanofiltration units under criticaltransmembrane pressure conditions. Chemical engineering research and design, 117, 488–499.

Curtin, L.V. (1983). Molasses-General Consideration, National Feed Ingredients Association West Des Moines, Iowa. (Accessed 28 December 2018, http://rcrec-ona.ifas.ufl.edu/media/rcrec-onaifasufledu/pdf/Molasses---General-Considerations.pdf)

Eckera, J., Raabb, T. & Haraseka, M. (2012). Nanofiltration as key technology for the separation of LA and AA. Journal of Membrane Science, 389, 389–398.

El-Geddawy, D., Mennat-Allah, M.A., Omar, M.B., seleim, M.M. & Elsyiad, S.I. (2012). Composition and properties of Egyptian beet molasses. Journal of Food and Dairy Sciences, 3(12), 669-679.

Fabiani, A., Versari, A., Parpinello, G.P., Castellari, M. & Galassi, S. (2002). High-performance liquid chromatographic analysis of free amino acids in fruit juices using derivatization with 9-Fluorenylmethyl-Chloroformate. Journal of Chromatographic Science, 40, 14–18.

Ferry, J.D. (1936). Ultrafilter and ultrafiltration, Chem. Rev. 11, 376-455.

Filipcev, B., Misan, A., Saric, B. & Simurina, O. (2016). Sugar beet molasses as an ingredient to enhance the nutritional and functional properties of glutenfree cookies. International Journal of Food Sciences and Nutrition, 67(3), 1-8.

Garem, A., Daufin, G., Maubois, J. & Leonil, J. (1996). Selective separation of amino acids with charged inorganic nanofiltration membrae: effect of physicochemical parameters on selectivity. Biotechnology and Bioengineering, 54(4), 291–302.

Geanta, R.M., Ruiz, M.O. & Escudero, I. (2013). Micellar-enhanced ultrafiltration for the recovery of lactic acid and citric acid from beet molasses with sodium dodecyl sulphate. Journal of Membrane Science, 430, 11-23.

 Gómez-Díaz, D., Navaza, J.M. & Quintáns-Riveiro, L.C. (2009). Effect of temperature on the viscosity of honey. International Journal of Food Properties, 12(2), 396-404.

 Gotoh, T., Iguchi, H. & Kikuchi, K.I. (2004). Separation of glutathione and its related amino acids by nanofiltration. Biochemical Engineering Journal, 19, 165–170.

 Goulas, A.K., Kapasakalidis, P.G., Sinclair, H.R., Rastall, R.A. & Grandison, A.S. (2002). Purification of oligosaccharides by nanofiltration. Journal of Membrane Science, 209, 321–335.

 Grib, H., Persin, M., Gavach, C., Piron, D.L., Sandeaux, J. & Mameri, N. (2000). Amino acid retention with alumina γ nanofiltration membrane. Journal of Membrane Science, 172, 9–17.

  Guan, Y., Tang, Q., Fu, X., Yu, S., Wu, S. & Chen, M. (2014). Preparation of antioxidants from sugarcane molasses. Food Chemistry, 152, 552–557.

  Guo, S., Luo, L., Wu, Y., Qi, B., Chen, X. & Wan, Y. (2018). Decolaration of sugar cane molasses by tight ultrafiltration: Filtration behavior and fouling control. Separation and Purification Technology, 204, 66-74.

  Gyura, J., Seres, Z., Vatai, G. & Molnar, E.B. (2002). The separation of non-sucrose compounds from the syrup of sugar beet processing by ultra and nanofiltration using polymer membrane. Desalination, 148, 49-56.

 Hong, S.U. & Bruening, M.L. (2006). Separation of amino acid mixtures using multilayer polyelectrolyte nanofiltration membranes. Journal of Membrane Science, 280, 1–5.

 Hong, S.U., Miller, M.D. & Bruening, M.L. (2006). Removal of Dyes, Sugars, and Amino Acids from NaCl Solutions Using Multilayer Polyelectrolyte Nanofiltration Membranes. Industrial & Engineering Chemistry Research, 45, 6284-6288.

  Hubbard, A.J. & Binder, D.K. (2016). Glutamate metabolism, In: A.J. Hubbard, D.K. Binder (Eds.), Astrocytes and Epilepsy, eds., Academic press. Cambridge Massachusetts. pp. 197–224.

  Ingole, P.G., Bajaj, H.C. & Singh, K. (2014). Membrane separation processes: Optical resolution of lysine and asparagine amino acids. Desalination, 343, 75–81.

  Katz, E.P. (1968). Molecular weight determination from amino acid analysis data: A numerical method. Analytical Biochemistry, 25, 417-431.

  Khan, S.H., Rasool, G. & Nadeemm, S. (2006). Bioconversion of cane molasses into amino acids. Pakistan Journal of Agricultural Sciences, 43, 157–161.

 Kovacs, Z. & Samhaber, W. (2008). Contribution of pH dependent osmotic pressure to amino acid transport through nanofiltration membranes. Separation and Purification Technology, 61, 243–248.

  Kovacs, Z. & Samhaber, W. (2009). Nanofiltration of concentrated amino acid solutions. Desalination, 240, 78-88.

 Lameloise, M.L. & Lewandowski, R. (1994). Purification of beet molasses by ionexclusion chromatography: fixed-bed modeling. Journal Chromatography A, 685, 45-52.

 Liu, M., Du, M., Zhang, Y., Xu, W., Wang, C., Wang, K. & Zhang, L. (2013). Purification and identification of an ACE inhibitory peptide from walnut protein. Journal of Agricultural and Food Chemistry, 61, 4097-4100.

Liu, L., Xie, X., Zambare, R.S. Selvaraj, A.P.J., Sowrirajalu, B.N., Song, X., Tang, C.Y. & Gao, C. (2018). Functionalized Graphene Oxide Modified Polyethersulfone Membranes for Low-Pressure Anionic Dye/Salt Fractionation. Polymers, 10(795), 2-15.

 Martin-Orue, C., Bouhallab, S. & Garem, A. (1998). Nanofiltration of amino acid and peptide solutions: mechanisms of separation. Journal of Membrane Science, 142, 225-233.

 Mee, J.M.L., Brooks, C.C. & Stanley R.W. (1979). Amino acid and fatty acid composition of cane molasses. Journal of the Science of Food and Agriculture, 30, 429–432.

 Muir, B., Quick, S., Slater, B.J., Cooper, D.B., Moran, M.C., Timperley, C.M., Carrick, W.A. & Burnell, C.K. (2005). Analysis of chemical warfare agents: II. Use of thiols and statistical experimental design for the trace level determination of vesicant compounds in air samples. Journal Chromatography A, 1068, 315–326.

 Munir, A. (2006). Dead End Membrane Filtration ENE 806 Laboratory Feasibility Studies in Environmental Engineering. (Accessed 28 December 2018, https://www.egr.msu.edu/~hashsham/courses/ene806/docs/Membrane%20Filtration.pdf.

 Rearik, D.E. & Mckey, C. (1996). The behavior of amino acids in chromatographic molasses desugarization, Conference on Sugar Process Research. (Accessed 28 December 2018), http://www.arifractal.com/images/files/The-behavior-of-amino-acids-in-chromatographic-molasses-desugarization-systems.pdf.

 Rodríguez, I., Amado, J.A., Vázquez, M.P. & González, M.A. (2013). Production of antihypertensive and antioxidant activities by enzymatic hydrolysis of protein concentrates recovered by ultrafiltration from cuttlefish processing wastewaters. Biochemical Engineering Journal, 76, 43-54.

Saidi, S., Deratani, A., Ben Amar, R. & Belleville, M.P. (2013). Fractionation of a tuna dark muscle hydrolysate by a two-step membrane process. Separation and Purification Technology, 108, 28-36.

Saric, L.C., Filipcev, B.V., Simurina, O.D., Plavsic, D.V., Saric, B.M., Lazarevic, J.M. & Milovanovic, I.L. (2016). Sugar beet molasses: properties and applications in osmotic dehydration of fruits and vegetables. Food and Feed Research, 43, 135–144.

Sato, N., Quitain, A.T., Kang, K., Daimon, H. & Fujie, K. (2004). Reaction Kinetics of Amino Acid Decomposition in High-Temperature and High-Pressure Water. Industrial and Engineering Chemistry Research, 43, 3217-3222.

 Sharma, R.R. & Chellam, S. (2005). Temperature effects on the morphology of porous thin film composite nanofiltration membranes. Environmental Science and Technology, 39, 5022-5030.

 Shim, Y., Rixey, W.G. & Chellam, S. (2008). Influence of sorption on removal of tryptophan and phenylalanine during nanofiltration. Journal of Membrane Science, 323, 99–104.

Tanaka, F., Tanaka, A. & Uchino, T. (2017). Effect of high temperature drying on amino acids decomposition in feed rice. Engineering in Agriculture, Environment and Food, 10, 1-3.

Thuy, C.X., Lam, T.B. & Commick, K.M. (2014). Optimizing of nanofiltration to obtain fish protein isolate (FPI) from Pangasius Hypophthalmus byproducts with calcium-binding bioactivity. Global Journal of Agricultural Research, 2(1), 11-21.

Timmer, J.M.K., Speelmans, M.P.J. & van der Horst, H.C. (1998). Separation of amino acids by nanofiltration and ultrafiltration membranes. Separation and Purification Technology, 14, 133–144.

Tsuru, T., Shutou, T., Nakao, S. & Kimura, S. (1994). Peptide and amino acid separation with nanofiltration membranes. Separation Science and Technology, 29(8), 971–984.

Valli, V., Gomez-Caravaca, A.M., Nunzio, M. D., Danesi, F., Caboni, M.F. & Bordoni, A. (2012). Sugar cane and sugar beet molasses, antioxidant-rich alternatives to refined sugar. Journal of Agricultural and Food Chemistry, 60, 12508-12515.

Varaee, M., Honarvar, M., Eikani, M.H., Omidkhah, M.R. & Moraki, N. (2019). Supercritical fluid extraction of free amino acids from sugar beet and sugar cane molasses. The Journal of Supercritical Fluids, 144, 48–55.

Vyas, B.B. & Ray, P. (2015). Preparation of nanofiltration membranes and relating surface chemistry with potential and topography: Application in separation and desalting of amino acids. Desalination, 362, 104–116.

Wang, G., Zhang, C., Sun, M., Zhang, X., Wu, C. & Wu, Y. (2017). Separation of mixed amino acids by BMED process using porous SPES and SPSF cation exchange membranes. 188, 539-547.

Wang, X.L., Ying, A.L. & Wang, W.N. (2002). Nanofiltration of l-phenylalanine and l-aspartic acid aqueous solutions. Journal of Membrane Science, 196, 59–67.

Wang, X.L., Tsuru, T., Togoh, M., Nakao, S. & Kimura, S. (1995). Evaluation of pore structure and electrical properties of nanofiltration membranes. Journal of Chemical Engineering of Japan, 28, 186–192.

Wang, X.L., Tsuru, T., Nakao, S. & Kimura, S. (1997). The electrostatic and steric-hindrance model for the transport of charged solutes through nanofiltration membranes. Journal of Membrane Science, 135, 19–32.

Wang, X.L., Tsuru, T., Togoh, M., Nakao, S. & Kimura, S. (1995). Transport of organic electrolytes with electrostatic and sterichindrance effects through nanofiltration membranes. Journal of Chemical Engineering of Japan, 28, 372–380.

Weiss, I.M., Muth, C., Drumm, R. & Kirchner, H.O.K. (2018). Thermal decomposition of the amino acids glycine, cysteine, aspartic acid, asparagine, glutamic acid, glutamine, arginine and histidine. BMC Biophysics, 11(2), 2-15.

Wilkes, R. & Jenning, R.P. (1971). A routine method for measuring molasses viscosity,   Proceedings of the South African Sugar Technologists Association. 107-113. (Accessed 28 December 2018, https://pdfs.semanticscholar.org/33dd/5d4f8bd9ebe9ed7e0aac0c13d119c15198b8.pdf.

Wu, Q., Du, J., Jia, J. & Kuang, C. (2016). Production of ACE inhibitory peptides from sweet sorghum grain protein using alcalase: Hydrolysis kinetic, purification and molecular docking study. Food Chemistry, 199, 140-149.

Wu, M.H. & Zhang, Y.Q. (2014). Nanofiltration recovery of sericin from silk processing waste and synthesis of a lauroyl sericin-based surfactant and its characteristics, RSC Advances, 4, 4140-4145.

York, M.J. (2017). Clinical pathology, In: A.S. Faqi (Ed.), A Comprehensive Guide to Toxicology in Nonclinical Drug Development (Second Edition), eds., Academic press. Cambridge Massachusetts. pp. 325–374.

Zeman, L. & Wales, M. (1981). Steric rejection of polymeric solutes by membranes with uniform pore size distribution. Separation Science and Technology, 16, 275-290.