بررسی فراوانی و مقاومت آنتی¬بیوتیکی ژن¬های حدت کمپیلوباکتر ژژونای جدا شده از گوشت دام و طیور عرضه شده در شهرستان شهرکرد
علی بهنیا
1
(
دانشجوی کارشناسی ارشد بهداشت مواد غذایی
)
سید مجید هاشمی
2
(
گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه آزاد شهرکرد، ایران.
)
کلید واژه: کمپیلوباکتر ژژونای, گوشت دام, گوشت طیور, مقاومت آنتی¬بیوتیکی ,
چکیده مقاله :
کمپیلوباکتریوز یک بیماری شایع گوارشی در سراسر جهان است. کمپیلوباکتر ژژونای در کشورهای صنعتی نیز بر سلامت عمومی تأثیر می-گذارند، لذا هدف از پژوهش حاضر، بررسی فراوانی و مقاومت آنتی¬بیوتیکی ژن¬های حدت کمپیلوباکتر ژژونای جدا شده از گوشت دام و طیور عرضه شده در شهرستان شهرکرد می¬باشد. ابتدا 272 نمونه گوشت و مرغ به صورت تصادفی از بازار عرضه این محصولات از شهرستان شهرکرد گردآوری و به آزمایشگاه انتقال داده شد، سپس توسط روش¬های استاندارد جهت ردیابی آلودگی¬ها و ارزیابی مقاومت آنتی¬بیوتیکی جدایه¬ها اقدام شد. نتایج نشان داد میانگین آلودگی به کمپیلوباکتر ژژونای در نمونه¬ها 68/53 درصد بود. به این ترتیب 43 نمونه (2/63 درصد) برای گوشت مرغ بسته¬بندی¬شده¬، 48 نمونه (6/70 درصد) گوشت مرغ بدون بسته¬بندی از 100 نمونه گوشت تازه بدون بسته¬بندی 34 نمونه (34 درصد) و از 36 نمونه گوشت چرخ¬شده، 23 نمونه (9/63 درصد) آلوده به کمپیلوباکتر ژژونای بودند. بیشترین و کمترین فراوانی ژن در گوشت چرخ¬شده به flaA ، 2/65 درصد و cadF با 13 درصد بود. نتایج ارزیابی مقاومت آنتی¬بیوتیکی نشان داد بیشترین مقاومت مربوط به سیپروفلوکساسین 94/97 درصد و کمترین مقاومت مربوط به امی¬پنم 58/9 درصد بود. نتایج این پژوهش نشان داد انواع گوشت¬ها می¬توانند از مخازن آلودگی به کمپیلوباکتر ژژونای باشند که رعایت اصول بهداشتی در کشتارگاه¬ها و مراکز عرضه می¬تواند کمک فزاینده¬ای به کاهش و یا حذف آلودگی کند.
چکیده انگلیسی :
Campylobacteriosis is a common disease worldwide. Campylobacter jejuni is also used in industry for public health. The purpose of this research is to investigate the frequency and antibiotic resistance of Campylobacter jejuni virulence genes isolated from livestock and poultry meat in Shahrekord city. First, 272 meat and chicken samples were randomly collected from the market of these products in Shahrekord city and transferred to the laboratory, then standard methods were used to track the harvests and evaluate the antibiotic resistance of the isolates. The results showed that the determination of Campylobacter jejuni in the samples was 53.68%. In this way, 43 samples (63.2 %) for packaged meat, 48 samples (70.6 %) for chicken meat without packaging, out of 100 samples of fresh meat without packaging, 34 samples (34 %) And out of 36 minced meat samples, 23 samples (63.9%) were infected with campylobacter jejuni. The most and least genes in minced meat were flaA, 65.2% and cadF, 13%. The results showing the resistance showed that the resistance to ciprofloxacin was 97.94% and the lowest resistance to imipenem was 9.58%. The results of this research showed that all types of meats can be reservoirs for Campylobacter jejuna, which can help to reduce or eliminate consumption by following the hygiene principles in slaughterhouses and centers.
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