Effect of Methanolic Extract of Pomegranate Peel on in vitro Rumen Fermentation Kinetics of Oil Seed Meals
محورهای موضوعی : Camel
1 - Young Researchers and Elite Club, Shabestar Branch, Islamic Azad University, Shabestar, Iran
2 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
کلید واژه: gas production, Fermentation, methanolic extract, oil seed meals, pomegranate peel,
چکیده مقاله :
This study was carried out to determine the effects of supplementing different levels (0, 0.5 and 1% of buffered rumen fluid) of methanolic extract of pomegranate peel, on rumen fermentation kinetics of four oil seed meals (soybean meal, cotton seed meal, rapeseed meal and sunflower seed meal), using in vitro gas production technique. The samples were incubated in syringes containing rumen liquor taken from three fistulated Iranian Ghezel rams for 2, 4, 6, 8, 12, 24 and 36 h. Results showed that, addition of methanolic extract of pomegranate peel led to significant increase in gas production volume in all incubation times and all of oil seed meals as well. Amount of gas production, also increased by increasing dose of the extract. Also, amounts of a (the gas production from the immediately soluble fraction), b (the gas production from the insoluble fraction) and a + b (the potential gas production) in all of tested oil seed meals increased by increasing pomegranate peel extract doses. Adding pomegranate peel extract resulted in increase volatile fatty acids (VFA) production. Production of VFA increased significantly by the level of the extract supplementation. In conclusion, it is suggested that, adding methanolic extract of pomegranate peel can be lead to higher ruminal fermentation and VFA production in ruminants.
این مطالعه به منظور تعیین اثرات افزودن عصاره متانولی پوست انار در سطوح 0، 5/0 و 1 درصد شیرابه شکمبه بافر شده بر روند تخمیر شکمبهای چهار نوع کنجاله دانههای روغنی شامل کنجاله سویا، کنجاله تخم پنبه، کنجاله منداب و کنجاله آفتابگردان، با استفاده از فن تولید گاز آزمایشگاهی به اجرا در آمد. نمونههای آزمایشی در سرنگهای حاوی شیرابه شکمبه گرفته شده از سه رأس گوسفند نر قزل کانولا گذاری شده، برای مدت زمانهای 2، 4، 6، 8، 12، 24 و 36 ساعت انکوبه شدند. افزودن عصاره متانولی پوست انار موجب افزایش معنیدار حجم گاز تولیدی در تمام زمانهای انکوباسیون و برای تمامی کنجالههای مورد آزمایش شد. همچنین مقدار گاز تولیدی متناسب با افزایش سطح عصاره افزایش یافت. مقدار فراسنجههای a(حجم گاز حاصل از تخمیر بخش سریع قابل حل)، b (حجم گاز حاصل از تخمیر بخش نامحلول اما قابل تخمیر) و مجموع گاز تولیدی (a+b)، نیز در تمامی کنجالههای مورد آزمایش، با افزایش دوز عصاره پوست انار افزایش یافت. افزودن عصاره پوست انار، تولید اسیدهای چرب فرار در شکمبه را نیز افزایش داد. متناسب با زیادتر شدن سطح عصاره، افزایش تولید اسیدهای چرب فرار نیز بیشتر شد. به عنوان یک نتیجه کلی، به نظر میرسد افزودن عصاره متانولی پوست انار به شیرابه شکمبه باعث افزایش تخمیر شکمبهای و تولید اسیدهای چرب بیشتر در نشخوارکنندگان میشود.
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