Effect of Methanolic Extract of Pomegranate Peel on in vitro Rumen Fermentation Kinetics of Oil Seed Meals
الموضوعات :
1 - Young Researchers and Elite Club, Shabestar Branch, Islamic Azad University, Shabestar, Iran
2 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
الکلمات المفتاحية: gas production, Fermentation, methanolic extract, oil seed meals, pomegranate peel,
ملخص المقالة :
This study was carried out to determine the effects of supplementing different levels (0, 0.5 and 1% of buffered rumen fluid) of methanolic extract of pomegranate peel, on rumen fermentation kinetics of four oil seed meals (soybean meal, cotton seed meal, rapeseed meal and sunflower seed meal), using in vitro gas production technique. The samples were incubated in syringes containing rumen liquor taken from three fistulated Iranian Ghezel rams for 2, 4, 6, 8, 12, 24 and 36 h. Results showed that, addition of methanolic extract of pomegranate peel led to significant increase in gas production volume in all incubation times and all of oil seed meals as well. Amount of gas production, also increased by increasing dose of the extract. Also, amounts of a (the gas production from the immediately soluble fraction), b (the gas production from the insoluble fraction) and a + b (the potential gas production) in all of tested oil seed meals increased by increasing pomegranate peel extract doses. Adding pomegranate peel extract resulted in increase volatile fatty acids (VFA) production. Production of VFA increased significantly by the level of the extract supplementation. In conclusion, it is suggested that, adding methanolic extract of pomegranate peel can be lead to higher ruminal fermentation and VFA production in ruminants.
Abarghuei M.J., Rouzbehan Y., Salem A.Z.M. and Zamiri M.J. (2013). Nutrient digestion, ruminal fermentation and performance of dairy cows fed pomegranate peel extract. Livest. Sci. 157, 452-461.
Abarghuei M.J., Rouzbehan Y. and Salem A.F. (2014a). The influence of pomegranate-peel extracts on in vitro gas production kinetics of rumen inoculum of sheep. Turkish J. Vet. Anim. Sci. 38, 212-219.
Abarghuei M.J., Rouzbehan Y., Salem A.Z.M. and Zamiri M.J. (2014b). Nitrogen balance, blood metabolites and milk fatty acid composition of dairy cows fed pomegranate-peel extract. Livest. Sci. 164, 72-80.
Benchaar C., Calsamiglia S., Chaves A.V., Fraser G.R., Colombatto D., McAllister T.A. and Beauchemin K.A. (2008). A review of plant-derived essential oils in ruminant nutrition and production. Anim. Feed Sci. Technol. 145, 209-228.
Calsamiglia S., Busquet M., Cardozo P.W., Castillejos L. and Ferret A. (2007). Invited review: essential oils as modifiers of rumen microbial fermentation. J. Dairy Sci. 90, 2580-2595.
Canbolat Ö., kamalak A. and Kara H. (2014). The effects of urea supplementation on pomegranate pulp (Punica granatum) silage fermentation, aerobic stability and in vitro gas production. Ankara Üniv. Vet. Fak. Derg. 61, 217-223.
Castillejos L., Calsamiglia S. and Ferret A. (2006). Effect of essential oils active compounds on rumen microbial fermentation and nutrient flow in in vitro systems. J. Dairy Sci. 89, 2649-2658.
Chen X.B. (1995). "Fitcurve" Macro. IFRU, the Macaulay Institute, Aberdeen, UK.
Delavar M.H., Tahmasbi A.M., Danesh-Mesgaran M. and Valizadeh R. (2014). In vitro rumen fermentation and gas production: influence of different by-product feedstuffs. Ann. Res. Rev. Biol. 4(7), 1121-1128.
Ebrahimi B., Taghizadeh A., Mehmannavaz Y. and Palangi V. (2012). Evaluation of pomegranate pomace using in situ and gas production techniques. J. Environ. Sci. Eng. A. 1, 951-955.
FAO/WHO. (2009). Project document for a regional standard for Pomegranate. FAO/WHO Coordinating Committee for the Near East. Tunis, Tunisia.
Feizi R., Ghodratnama A., Zahedifar M., Danesh Mesgaran M. and Raisianzadeh M. (2005). Apparent digestibility of pomegranate seed fed to sheep. J. Dairy Sci. 88(1), 194-203.
Halimi Shabestari A., Salamatdoustnobar R., Maheri-Sis N., Gorbani A., Mirzadeh Ahari K., Noshadi A., Samadi H. and Salimi Nezhad J. (2011). Evaluation effects of clove methanol extract on methane production in the in vitro condition. Pakistan J. Nutr. 10(12), 1154-1157.
Hassanpour S., Maheri-Sis N., Eshratkhah B. and Baghbani Mehmandar F. (2011). Plants and secondary metabolites (Tannins): a review. Int. J. Forest. Soil. Erosion. 1(1), 47-53.
Jami E., Shabtay A., Nikbachat M., Yosef E., Miron J. and MizrahiI. (2012). Effects of adding a concentrated pomegranate-residue extract to the ration of lactating cows on in vivo digestibility and profile of rumen bacterial population. J. Dairy Sci. 95, 5996-6005.
KilicU., Boga M., Gorgulu M. and Şahan Z. (2011). The effects of different compounds in some essential oils on in vitro gas production. J. Anim. Feed Sci. 20, 626-636.
Kohansal M.R. and Rahimi M. (2013). Investigating factors marketing pomegranate in Fars. Int. J. Agron. Plant. Prod. 4(10), 2759-2763.
Maheri-Sis N., Chamani M., Sadeghi A.A., Mirza-Aghazadeh A. and Aghajanzadeh-Golshani A. (2008). Nutritional evaluation of kabuli and desi type chickpeas (Cicer arientinum) for ruminants using in vitro gas production technique. African J. Biotechnol. 7(16), 2946-2951.
Makkar H.P.S. (2005). In vitro gas methods for evaluation of feeds containing phytochemicals. Anim. Feed Sci. Technol. 123, 291-302.
Maleki Baladi R., Moghaddaszadeh-Ahrabi S. and Afrouziyeh M. (2014). Influence of the addition of different levels of tannin extracted from pomegranate pomace, on some nutritive value of soybean meal. European J. Exp. Biol. 4(2), 148-154.
Manuel Viuda-Martos J.A., Pérez-álvarez E.S. and Fernández-López J. (2013). In vitro antioxidant properties of pomegranate (Punica granatum) peel powder extract obtained as coproduct in the juice extraction process. J. Food Proc. Pres. 37(5), 772-776.
Menke K.H. and Steingass H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28, 7-55.
Mirzadeh Ahari K., Salamatdoustnobar R., Maheri-Sis N., Gorbani A., Halimi Shabestari A., Noshadi A., Samadi H. and Salimi Nezhad J. (2011). Effects of the thyme extract on the ruminal methane production. Pakistan J. Nutr. 10(12), 1146-1148.
Mirzaei-Aghsaghali A. and Maheri-Sis N. (2008). Nutritive value of some agro-industrial by-products for ruminants: a review. World J. Zool. 3(2), 40-46.
Mirzaei-Aghsaghali A., Maheri-Sis N., Mansouri H., Razeghi M.E., Mirza-Aghazadeh A., Cheraghi H. and Aghajanzadeh-Golshani A. (2011). Evaluating potential nutritive value of pomegranate processing by-products for ruminants using in vitro gas production technique. ARPN J. Agric. Biol. Sci. 6(6), 45-51.
Modaresi J., Fathi Nasri M.H., Rashidi L., Dayani O. and Kebreab E. (2011). Effects of supplementation with pomegranate seed pulp on concentrations of conjugated linoleic acid and punicic acid in goat milk. J. Dairy Sci. 94, 4075-4080.
Negi P.S. and Jayaprakasha G.K. (2003). Antioxidant and antibacterial activities of Punica granatum peel extracts. J. Food Sci. 68(4), 1473-1477.
Nezarati S., Maheri-Sis N., Salamatdoust-Nobar R., Aghajanzadeh-Golshani A. (2014). In vitro fermentation characteristics and nutritive value of Iranian oil seed meals for ruminants. Greener J. Biol. Sci. 4(2), 53-58.
Niasati M., Palizdar M.H., Pourelmi M.R. and Pasha Chalandari H. (2014). Effect of methanolic extract of Viscum album on in vitro fermentation and digestibility of soybean meal. Res. Opin. Anim. Vet. Sci. 4(7), 411-415.
Olaniyi A.F., Nokwanda P.M. and Umezuruike L.O. (2012). Antibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extract. BMC Comp. Altern. Med. 12, 1-11.
Oliveira R.A., Narciso C.D., Bisinotto R.S., Perdomo M.C., Ballou M.A. and Dreher M. (2010). Effects of feeding polyphenols from pomegranate extract on health, growth, nutrient digestion, and immunocompetence of calves. J. Dairy Sci. 93, 4280-4291.
Ørskov E.R. and McDonaldI. (1979). The estimation of protein degradability in the rumen from incubation measurements weighed according to rate of passage. J. Agric. Sci. 92, 499-503.
Pashachalandari H., Palizdar M.H., Mohammadian-Tabrizi H.R. and Niasati M. (2014). Effect of methanolic extract of nettle (Urtica dioica) on in vitro fermentation and gas production of canola meal. Res. Opin. Anim. Vet. Sci. 4(7), 416-419.
Refat B., AneleU., He Z.X., Bassiony S.M., Abdel-Rahman G.A. and Yang W.Z. (2015). Effect of sainfoin hay and pomegranate peel extracts on in vitro fermentation and protein degradation using the Rusitec technique. Canadian J. Anim. Sci.
Rezaei N., Salamat doust-Nobar R., Maheri Sis N., Salamatazar M., Namvari M., Goli S. and Aminipour H. (2011). Evaluation effect of some plant extracts on degradability of soybean meal with gas product technique. Ann. Biol. Res. 2(4), 224-228.
SAS Institute. (1991). SAS®/STAT Software, Release 6.0. SAS Institute, Inc., Cary, NC. USA.
Salamat Azar M., Najafyar S., Rezaei N. and Aminipour H. (2011). Zataria multiflora extract affect in vitro gas producing techniques rumen microbial fermentation. Ann. Biol. Res. 2(5), 632-636.
Salamat Azar M., Salamat Doust-Nober R., Maheri Sis N., Aghdam Shahryar H. and Asadi Y. (2012). Effects of zataria multiflora extract as rumen modifiers using in vitro gas production technique. Curr. Res. J. Biol. Sci. 4(4), 350-354.
Salamatazar M., Salamatdoust-nobar R. and Maheri Sis N. (2012). Evaluation of the effects of Thymus vulgar on degradability kinetics of canola meal for ruminant using in vitro gas production technique. J. Cell. Anim. Biol. 6(11), 164-168.
Salamatazar M., Salamatdoustnobar R., Asadi Y., Maheri Sis N., Najafyar S., Khodaparast B., Aminipour H., Rezayi N. and Maleki M. (2011). Effect of thyme methanolic extract on degradability kinetics of sunflower meal. Ann. Biol. Res. 2(4), 104-109.
Shabtay A., Eitam H., Tadmor Y., Orlov A., Meir A., Weinberg P., Weinberg Z.G., Chen Y., Brosh A., Izhaki I. and Kerem Z. (2008). Nutritive and antioxidative potential of fresh and stored pomegranate industrial byproduct as a novel beef cattle feed. J. Agric. Food Chem. 56, 10063-10070.
Shabtay A., Nikbachat M., Zenou A., Yosef E., Arkin O., Sneer O., Shwimmer A., Yaari A., Budman E., Agmon G. and Mirona J. (2012). Effects of adding a concentrated pomegranate extract to the ration of lactating cows on performance and udder health parameters. Anim. Feed Sci. Technol. 175, 24-32.
Sirohi S.K., Goel N. and Pandey P. (2012a). Efficacy of different methanolic plant extracts on anti-methanogenesis, rumen fermentation and gas production kinetics in vitro. Open. Vet. J. 2, 72-77.
Sirohi S.K., Mehta M., Goel N. and Pandey P. (2012b). Effect of herbal plants oil addition in total mixed diets on anti-methanogenic activity, rumen fermentation and gas production kinetics in vitro. J. Nat. Prod. Plant Resour. 2(1), 73-80.
Steel R.G.D. and Torrie J.H. (1980). Principles and Procedures of Statistics: A Biometrical Approach. McGraw Hill, New York, USA.
Taher-Maddah M., Maheri-Sis N., Salamatdoustnobar R. and Ahmadzadeh A. (2012a). Comparing nutritive value of ensiled and dried pomegranate peels for ruminants using in vitro gas production technique. Ann. Biol. Res. 3(4), 1942-1946.
Taher-Maddah M., Maheri-Sis N., Salamatdoustnobar R. and Ahmadzadeh A. (2012b). Estimating fermentation characteristics and nutritive value of ensiled and dried pomegranate seeds for ruminants using in vitro gas production technique. Open. Vet. J. 2, 40-45.
Tajodini M., Moghbeli P., Saeedi H.R. and Effati M. (2014). The effect of medicinal plants as a feed additive in ruminant nutrition. Iranian J. Appl. Anim. Sci. 4(4), 681-686.
