• List of Articles lamiaceae

      • Open Access Article

        1 - A review on pharmacological potentials of phenolic diterpenes carnosic acid and carnosol obtained from Rosmarinus officinalis L. and modern extraction methods implicated in their recovery
        Dhananjay Singh Nishu Mittal Mohammed Siddiqui
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        2 - Chemical compositions of the essential oils in Salvia sahendica Boiss. & Buhse at two different growth stages
        A. A Majrouhi,
        Salvia, the largest genus of Lamiaceae, is represented in Iranian flora by 58 species, 17 of which are endemic. Some of these species have been used in folk medicine all around the world for their antibacterial and antitumor activities as well as flavoring agent in perf More
        Salvia, the largest genus of Lamiaceae, is represented in Iranian flora by 58 species, 17 of which are endemic. Some of these species have been used in folk medicine all around the world for their antibacterial and antitumor activities as well as flavoring agent in perfumery and cosmetics. The aerial parts of Salvia Sahendica were collected at vegetative and flowering stages from the Sahand Mountain near to Sardroud (Azarbayjan province, Iran). The essential oils were isolated by hydrodistillation Clevenger type apparatus and analyzed by GC and GC-MS. In vegetative stage, Thirty-two components were identified, representing 98.5 % of the total oil. α- pinene (28.5%), β- pinene (26.1%), sabinene (10.3%), germacrene- D (9.6%) and 1,8- cineole (4.9%) were the main components in vegetative stage. In flowering stage, Thirty-five components were identified, representing 99.8% of the total oil. α- pinene (18.9%), β- pinene (18.5%), 1,8- cineole (13.9%), linalyl acetate (8.4%), bicyclogermacrene (8.2%), sabinene (7.6%) and linalool (5.3%) were the main components at flowering stage. Manuscript profile
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        3 - Phylogenetic survey of chloroplast matK sequences in some medicinal plant species of Mentheae tribe (Lamiaceae) with emphasis on Zhumeria majdae Rech. & Wendelbo.
        Hamed Khodayari Elham Khalili fourogh Sanjarian Yunes Asri
        The aim of this research is elucidation of molecular taxonomic and phylogenetic relationship between Zhumeria and other genera of Mentheae tribe using sequencing of chloroplastic matk gene. The aim of this research is taxonomic and phylogenetic status of Mentheae tribe More
        The aim of this research is elucidation of molecular taxonomic and phylogenetic relationship between Zhumeria and other genera of Mentheae tribe using sequencing of chloroplastic matk gene. The aim of this research is taxonomic and phylogenetic status of Mentheae tribe with emphasis Zhumeria majdae Resh. f. & Wendelbo. (Moorkhosh) belonged to Lamiaceae family that endemic of Hormozgan province, Iran and it have not been reported in elsewhere, using sequencing of chloroplastic gene matK. Zhumeria majdae has long been used in traditional medicine as antispasmodic, antimicrobial, carminative especially in infants and for dysmenorrheal. The therapeutic benefits of medicinal plants are often attributed to their antioxidant properties. It is used for treatment of the stomach ulcer, headache, reduce pain, cold and healing of wounds. The methods are as follow: Extraction of DNA, amplification of a part of matk by specific primers, DNA sequencing, and sequence analysis by FINTCH TV software, drawing phylogenetic tree by PAUP software.  The heuristic search of the Cladogram analysis resulted in 10000 shortest trees, 3 tree with lengh of 20 steps and statistic coefficient (Retention index = 0.963) and Consistencies index=0.950. In the trees resulting from maximum parsimony and maximum likelihood methods, Mentheae tribe was monophyletic and composed of three distinct monophyletic clades (subtribes Menthinae, Salviinae and Nepetinae). The The tree derived from the combined data matrix, including in-group and out-group taxa, has a total of 74 sequences. results of this study showed that Zhumeria majdae grouped with Salvia clad. Our results comfirm previous studies done by morphological, trnL-F, psbA-trnH and ribosomal DNA phylogeny.   Manuscript profile
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        4 - Analysis and Identification of Essential Oil Constituents in the Vegetative and Reproductive Organs of Salvia macrosiphon Boiss. in Natural Habitat of Fars Province
        Ali Bahrami Alireza Yavari Alireza Raheb
        This study is the first to investigate the chemical composition of three organs (flower, leaf and stem) essential oils of Salvia macrosiphon Boiss., which grows wild and belongs to Lamiaceae family, in Fras procince. In the present experiment, 30 plants in full flowerin More
        This study is the first to investigate the chemical composition of three organs (flower, leaf and stem) essential oils of Salvia macrosiphon Boiss., which grows wild and belongs to Lamiaceae family, in Fras procince. In the present experiment, 30 plants in full flowering stage were randomly prepared from Jahrom region of Fars province and divided into three groups of 10 and then flowers, leaves and stems of each group were isolated for testing. Essential oil was extracted from each organ with three replications and in each repetition 200 g of plant material was done by hydro-distillation using Clevenger apparatus and they were analyzed by a combination of GC-FID and GC-MS techniques, to check for chemical variability. The yield of essential oil (w/w%) was the highest in flowers (0.48%) > then in leaves (0.28%) > and finally in stems (0.06%). The total number of compounds identified and quantified were twenty-five in flowers, eighteen in leaves and twenty-one in stems, representing 93.1%, 93.4% and 92.2% of the total oil, respectively. Results of essential oil compound analysis illustrated that flower expressed a high content of linalool. Meanwhile, bicyclogermacrene + (E)-caryophyllene and germacrene D + bicyclogermacrene were the major compounds in leaf and stem organs, respectively. Also, results showed that sesquiterpene hydrocarbons were the common and the highest amount in the three studied organs, which were the highest in leaves (69.1%) > then stems (68.0%) > and at last in flowers (34.5%). The highest level of oxygenated monoterpens was found at the flower part, represented by 27.3% of linalool. In conclusion, the plant organs of S. macrosiphon affected its essential oil quality and its concentration. Flowers were the most beneficial organ of this species for essential oil and linalool compound productions. Manuscript profile
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        5 - بررسی ترکیبات شیمیایی اسانس سه جنس از خانواده نعناع (Lamiaceae) در شمال ایران
        سالار حافظ قران بشرا آزادی هدایت حسین
        مقدمه و هدف: نعناع یا لابیاته (معمولاً تحت عنوان mint یا deadnettle نیز نامیده می­شود) خانواده­ای بسیار بزرگ و گسترده از گیاهان گلدار (Angiospermae) می­باشد. در طب عامیانه ایران از بیشتر گیاهان خانوادۀ نعناع به­عنوان محرک، ضدعفونی کننده، مقوی باء، درمان More
        مقدمه و هدف: نعناع یا لابیاته (معمولاً تحت عنوان mint یا deadnettle نیز نامیده می­شود) خانواده­ای بسیار بزرگ و گسترده از گیاهان گلدار (Angiospermae) می­باشد. در طب عامیانه ایران از بیشتر گیاهان خانوادۀ نعناع به­عنوان محرک، ضدعفونی کننده، مقوی باء، درمان معده، خلط­آوری، ضد نفخ، ضد اسهال و ضد باکتری استفاده می­شود. هدف از مطالعۀ حاضر، به­دست آوردن بینشی بهتر نسبت به طبیعت اسانس بود. روش تحقیق: اسانس­های سه جنس از خانوادۀ نعناع در شمال ایران (مرزه گل­درشت، کاکوتی ایرانی و اسطوخودوس فرانسوی) توسط روش تقطیر با آب از گل­های آن­ها گرفته شدند. مطالعه حاضر منجر به شناسایی 67 ترکیب توسط دستگاه­های کروماتوگرافی گازی با دتکتور یونش شعله­ای و کروماتوگرافی گازی با دتکتور طبف­سنجی جرمی شد؛ و بازده اسانس­ها به ترتیب برابر با 1.3%، 1.64% و 1.16% از گل­های تازه محاسبه شد. نتایج: کارواکرول (43.3%)، گاما-ترپینن (24.1%) و پاراسیمن (21.6%) به­عنوان ترکیب­های شیمیایی اصلی اسانس مرزه گل­درشت شناسایی شدند. به عبارت دیگر از کل ترکیب­های اسانس کاکوتی ایرانی (98%)،عمده ترکیب­های شیمیایی شناسایی شده می­توان به ایزوپولگون (46.6%) و همچنین پارا-منتا-1(7)و8-دی ان (16.4%)، 1و8-سینئول (7.0%)، ایزومنتول (5.5%) و ایزولدن (3.3%) اشاره کرد. کامفر (61.5%)، 1و8-سینئول (10.1%)، فنکون (4.2%) و لینالول (3.8%) از عمده ترکیب­های اسانس اسطوخودوس فرانسوی می­باشند. از اسانس­های مرزه گل­درشت، کاکوتی ایرانی و اسطوخودوس فرانسوی بیشترین مقدار مونوترپن­ها (به­ترتیب؛ 96.52%، 89.31% و 93.49%) و سزکوئی­ترپن­ها (به­ترتیب؛ 1.87%، 8.1% و 5.04%) شناسایی شدند. توصیه کاربردی/صنعتی: بر اساس نتایج به­دست آمده از این مطالعه، می­توان تنوع در ترکیب­های شیمیایی شناسایی شده از هر گیاه در مقایسهبا سایر مطالعات دیگر را به منطقه جغرافیایی و شرایط زیست محیطی (دما، میزان رطوبت، برهمکنش با سایر جنس­های دیگر و غیره) نسبت داد و شرایط ذکر شده نقش مهمی در بیوسنتز و اجزای ترکیب­های اسانسی خواهند داشت. Manuscript profile
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        6 - مروری بر جنس گیاه دارویی آویشن
        عبدالله قاسمی پیربلوطی زهره امامی بیستگانی فاطمه ملک پور
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        7 - اثر روش های مختلف اسانس گیری بر کمیت و کیفیت اسانس دو گونه از جنس کاکوتی (Ziziphora L)
        حسین بتولی مریم اخباری سید محمد جواد حسینی‌زاده
        مقدمه و هدف: جنس "کاکوتی"(Ziziphora L.) متعلق به خانواده نعناعیان(Lamiaceae)، دارای گونه‌های داروئی و معطره بسیار ارزشمندی است که تاکنون بالغ بر 25 گونه از این جنس در جهان و 4 گونه یک ساله و چند ساله از ایران گزارش شده ‌است. در این تحقیق ترکیب‌های شیمیائی اسانس دو گونه& More
        مقدمه و هدف: جنس "کاکوتی"(Ziziphora L.) متعلق به خانواده نعناعیان(Lamiaceae)، دارای گونه‌های داروئی و معطره بسیار ارزشمندی است که تاکنون بالغ بر 25 گونه از این جنس در جهان و 4 گونه یک ساله و چند ساله از ایران گزارش شده ‌است. در این تحقیق ترکیب‌های شیمیائی اسانس دو گونه­ی کاکوتی(Z. tenuior L.، Z. clinopodioides Lam.) منطقه قمصر کاشان مورد بررسی قرار گرفته‌ است. روش تحقیق: سرشاخه‌های گل‌دار گونه‌‌های مذکور در بهار سال 1390 جمع‌آوری و به روش های مختلف شامل تقطیر و استخراج با بخار هم­زمان با حلال آلی (SDE)، کلونجر، اسانس­گیری با بخار سرد (با استفاده از امواج فراصوت) و تقطیر با بخار آب اسانس‌گیری شدند. برای شناسائی ترکیب‌های تشکیل‌دهنده اسانس، از دستگاه‌های گاز کروماتوگرافی(GC) و گاز کروماتوگرافی متصل‌ شده به طیف‌سنج جرمی(GC/MS) استفاده شد. نتایج و بحث: عملکرد اسانس در روش‌های مختلف، در گیاه کاکوتی یا   Z. tenuior بین 2/0 تا 3/2% و در گیاه کاکوتی کوهی یا  Z. clinopodioides بین 3/0 تا 3/1% متغییر بود. 18 ترکیب شیمیائی در اسانس سرشاخه‌های گل‌دار گیاه کاکوتی و 33 ترکیب در اسانس گیاه کاکوتی‌کوهی شناسایی شدند. اجزای اصلی اسانس کاکوتی شامل پولگون (1/90%-0/80%) و پی­پریتنون (1/7%-5/4%) بودند. بیشترین ترکیب‌های تشکیل‌دهنده اسانس کاکوتی‌‌کوهی شامل پولگون (2/35%-9/25)، پی­پریتنون (9/27%-1/10%)، منتول(5/17%-4/11%) و منتون (7/7%-0) بودند. حضور درصد بالای منتول در اسانس گیاه کاکوتی‌کوهی، باعث مرغوبیت اسانس این گیاه شده ­است. بهترین راندمان اسانس در دو گیاه، متعلق به روش تقطیر و استخراج با بخار همزمان با حلال آلی بود. توصیه کاربردی/صنعتی: با توجه به خلوص 81 درصدی ترکیب پولگون در اسانس گیاه کاکوتی و با در نظر داشتن بازده حدوداً دو درصدی استحصال اسانس، می­توان در صورت کشت مکانیزه این گیاه، ترکیب مورد نظر را خالص سازی نمود. Manuscript profile
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        8 - Nutritional responses of Thymus exposed leaf spraying under soil nitrogen deficiency
        Zeinab Asle Mohammadi Nayer Mohammadkhani Moslem Servati
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        9 - The effect of selenium and silicon nanoparticles on increasing the growth and productivity of peppermint (Mentha piperita L.) under salinity stress
        Vahidreza Jalali Mahdi Javaheri
        Irrigation water salinity is one of the growing problems in the path of agricultural and horticultural production that researchers must determine different solutions to this problem and provide it to farmers. In this regard, silicon and selenium due to their positive ro More
        Irrigation water salinity is one of the growing problems in the path of agricultural and horticultural production that researchers must determine different solutions to this problem and provide it to farmers. In this regard, silicon and selenium due to their positive role, both structurally and as cofactors of enzymes related to the metabolism of various antioxidants can play an effective role in increasing plant tolerance to salinity stress. For this purpose, a factorial experiment was conducted in a completely randomized design including four salinity levels and three fertilizer sources of silicon and selenium compounds in normal and nano size in Shirvan Faculty of Agriculture, Bojnourd University. Statistical analysis showed that the main effect of salinity stress, as expected, had a significant reducing effects on all studied traits (except essential oil). In these variables, although the main effect of fertilizer type was not statistically significant, but at different levels of silicon and selenium consumption, an increasing trend of these variables was observed, indicating the positive effect of simultaneous use of these two elements in reducing the effect of salinity. Also the main effects of salinity and fertilizer sources on leaf length and width factors, fresh and dry weight of stem, number of sub-branches, number of leaves and leaf area index were significant. In all these indicators, the application of silicon and selenium elements in nano size has always been more effective than the normal size of these elements, which should be considered as a management strategy in salinity stress conditions. Manuscript profile
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        10 - Relative quantitation of hyphae-specific gene HWP1 expression in inhibition of Candida albicans biofilm
        Alireza Khodavandi Fahimeh Alizadeh Mozhdeh Shahinipor
        Background & Objectives: The incidence of candidiasis has been increased in immune compromised patients. Biofilm formation is counted as the main mechanisms of antibiotic resistance in Candida albicans. The aim of this study was to investigate the effects extraction More
        Background & Objectives: The incidence of candidiasis has been increased in immune compromised patients. Biofilm formation is counted as the main mechanisms of antibiotic resistance in Candida albicans. The aim of this study was to investigate the effects extractions of Lamiaceae family in quantification of HWP1 gene expression responsible for inhibition of biofilm formation in C. albicans. Materials & Methods: In this cross- sectional study, the antifungal effect of aqueous and ethanolic extractions of leaf, stem and root of Mentha spicata, Mentha pulegium and Thymus vulgar, commercially purchased from Yasooj, was analyzed against C. albicans using disc diffusion and broth microdilution methods. The crystal violet colorimetric method, morphological response and expression pattern of hypha-specific gene HWP1were carried out to investigate the biofilm-inhibitory properties of the best plant extract tested. Results: The data indicated that aqueous root extracts of Thymus vulgaris exhibits high antifungal activity against C. albicans. The aqueous extract of Thymus vulgaris root-treated cells exhibited significant reduction in biofilm growth. In addition, morphological observation of extract of Thymus vulgaris and fluconazole-treated cells confirmed decreases in fungal reproduction. Finally, aqueous root extractions of Thymus vulgaris was shown to down-regulate the expression of HWP1. Conclusion: The results of this study showed the possible molecular mechanism of effects of aqueous root extraction of Thymus vulgaris root in C. albicans on biofilm formation. Manuscript profile
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        11 - The effect of bio fertilizers on some growth parameters and essential oil of Moldavian dragonhead under drought conditions
        Hoseein Gorgini Shabankareh محمد رضا اصغری پور براتعلی فاخری
        One of the fundamental pillars in sustainable agriculture is use of bio-fertilizers in agro-ecosystems in order to eliminate or reduce the use of chemical inputs. The experimental design was a split plot with four irrigation regimes: irrigation at 40 %, 60 %, 80 % and 1 More
        One of the fundamental pillars in sustainable agriculture is use of bio-fertilizers in agro-ecosystems in order to eliminate or reduce the use of chemical inputs. The experimental design was a split plot with four irrigation regimes: irrigation at 40 %, 60 %, 80 % and 100% FC (as control) comprising the main treatments, and four biological fertilizers including nitroxin, super nitroplas and biophosphor and non-fertilizer (as control) as the sub -treatments that were applied with three replications. The experiment was conducted in 2013 at the Zabol University research farm in Zabol, south Iran. Nitroxin treated plants had the highest leaf relative water content, plant height, number of lateral branches, fresh and dry weight and followed by nitroplas and biophosphor. Plants irrigated at 80 % FC had the higher plant height, number of lateral branches, fresh and dry weight. While the greatest relative water content was achieved at irrigation at 100 % of FC (control). The experimental results suggested that application of nitroxin along with irrigation at 80 % FC could be useful in drought conditions. Manuscript profile
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        12 - The effect of mycorrhizal fungi, water stress and year on flower yield and some characteristics of medicinal plant of Borage (Borago officinalis L.) in Yasouj region
        Ali Rahimi
        Identifying the critical time and timing of plant irrigation based on a precise and basic plan is the key to water conservation, improvement of irrigation operations and plant tolerance to water shortage in agriculture. In recent years, vesicular arbuscular mycorrhizal More
        Identifying the critical time and timing of plant irrigation based on a precise and basic plan is the key to water conservation, improvement of irrigation operations and plant tolerance to water shortage in agriculture. In recent years, vesicular arbuscular mycorrhizal fungi have been used in many plants to deal with dehydration and drought stress. In this regard, an experiment was conducted as split-plot in the form of randomized complete block design with 3 replications in the Yasuj region at years 2015 and 2016. Water stress was considered as the main factor in the form of irrigation after 30, 60, 90, 120 and 150 mm of evaporation from the evaporation pan class A and mycorrhizal fungus was considered as a secondary factor in the form of no application, application of Glomus mosseae and application of Glomus intraradices. The results showed that the interaction of irrigation and mycorrhizal fungi on flower phosphorus, flower yield, biological yield and water use efficiency of Borage was significant. In irrigation levels after 60, 90, 120 and 150 mm of water evaporation from the evaporation pan, the use of mycorrhizal fungi Glomus mosseae and Glomus intraradices compared to the absence of fungi respectively increased the yield of plant flowers (30.04% and 27.35 %), (90.2% and 90.98%), (93.21% and 94.1%) and (81.73% and 78.86%), also, at these levels of irrigation, biological yield and water use efficiency of Borage flower achieved a significant increase in the presence of mycorrhizal fungus, compared to the absence of mycorrhizal fungus application. Application of both strains of mycorrhizal fungus Glomus mosseae and Glomus intraradices in irrigation levels after 90, 120 and 150 mm of water evaporation from the evaporation pan compared to no application of mycorrhizal fungus, respectively, resulted in a significant increase in the harvest index of Borage flower (44.55% and 43.36%), (13.21% and 15.96%) and (5.6% and 5.41%) and flower phosphorus (44.69% and 20.45%), (150% and 125%) and ( 267.74% and 235.48%). Mycorrhizal fungus was able to moderate the negative effects of drought stress and increase the above-mentioned traits in those irrigation levels, and based on the results of this study, irrigation treatment after 90 mm of water evaporation from the evaporation pan + the use of Glomus mosseae mycorrhizal fungi is recommended. Manuscript profile