study on period of persistence of foot-and-mouth disease virus in different organs in guinea pigs .After adaptation of foot-and-mouth disease viruses type O in 10 guinea pigs pad’s, 30 Animaldivided to 5 groups containing 6 animals in each group so that 5 animals More
study on period of persistence of foot-and-mouth disease virus in different organs in guinea pigs .After adaptation of foot-and-mouth disease viruses type O in 10 guinea pigs pad’s, 30 Animaldivided to 5 groups containing 6 animals in each group so that 5 animals inoculated from eachgroup and normal saline injected in a guinea pig as negative control.Euthanized animal in 5 periods including: 2 days post virus generalization٫ 4 days post virusgeneralization ٫14 days post virus generalization٫ 30 days post virus generalization٫ 60 days postvirus generalization. Then collecting samples from: heart٫ lung٫ liver, spleen٫ pancreas٫retropharyngeal lymph node, palntar epithelium, inguinal lymph node and tongue Epithelium, sentto Pathology Department to pathological studies also other of those sent to FMD Department to viraldetection. Pathological samples fixed in formalin then prepared paraffin ،s blocks and slides with5 microns with H&E staining. Viral samples kept in glycerinated buffer and detected the amount ofvirus by Sandwich Elisa according to reference. At least base on our study the maximum of FMDVlesions we observed affected plantar Epithelium٫ Lung٫ Tongue Epithelium and spleen consequentlyand showed that pathologic lesion contained: Intracellular spongiosis, vacuolization and pyknosis ofcells from the spinous layer, Intercellular edema in spinous layer of Epithelium (plantar and Tongue)also Hyperemia and increased size of inter alveolar septa in Lung and decreased in white pulpcompared to uninfected control guinea pig in spleen . In addition results of Elisa determined extremeamount of virus were in 14 days post virus generalization and we have not any evidence of beingvirus in 30 and 60 days post virus generalization. It is concluded that the results of Pathologicalobservations can be confirmed by Elisa detection.
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Inroduction & Objective: The importance of histophysiological and histochemical study of the uterus, cervix and vagina in mammals is due to pathological examinations of these organs in infectious and metabolic diseases and infertility treatment. The aim of this stud More
Inroduction & Objective: The importance of histophysiological and histochemical study of the uterus, cervix and vagina in mammals is due to pathological examinations of these organs in infectious and metabolic diseases and infertility treatment. The aim of this study was to investigate the histophysiological and histochemical features of these organs in guineapigs.Materials and Methods: Five female guinea pigs with mean weight were obtained from laboratory animal breeding center and after sampling, Samples were stained by Hematoxin - Eosin, Periodic acid Schiff and alcian blue methods and then histological and histochemical properties of samples were samples were studied by light microscope.Results: The horn and the body of the uterus in general have three layers of endometrium , myometrium and primetrium. The endometrical epithelium in the uterine horn consists of simple cubic cells and it consists of simple cubic cells and simple columnar cells in the body of uterus. The endometrium has many coiled tubular glands. In the body and horn, the myometrium has 2 layers of longi tudinal and circular smooth muscle from outside to inside. The cervical mucosa has simple columnar cells. And has 3 types of folds: primary, Secondary and tertiary folds. Among the columnal cells, a large number of mucus secreting goblet cells are seen. The squomous vaginal epithelium is stratified stratum Corneum. no glands were observed in mucosal layer of the vagina.Conclusion: The results show that the anatomical a tissue structure of Guinea pig uterus, cervix and vagina, despite minor differences, are very similar to other mammals.s.
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In the present study, a total number of 10 guinea pig eyes were studied macroscopically and microscopically (with staining by H&E, Verhoeff, Van Gieson, and P.A.S.). The results showed that posterior surface of lens was more convex than anterior surface. The average More
In the present study, a total number of 10 guinea pig eyes were studied macroscopically and microscopically (with staining by H&E, Verhoeff, Van Gieson, and P.A.S.). The results showed that posterior surface of lens was more convex than anterior surface. The average lens diameter and thickness were respectively measured as 0.61±0.02 and 0.45±0.024 centimeters. The average ciliary body thickness was measured as 0.25±0.03 centimeters. In addition, the ciliary body’s mean external diameter as 0.92±0.03 centimeters and the number of ciliary body process is about 68. The epithelium of lens was cuboid and the lens capsule as positive PAS reaction intensively. The anterior surface of capsule was thicker than posterior one. The lens fibers in the central part were thicker than other parts and the elastic fibers in lens were not observed. The epithelium of ciliary processes has two layers; the superficial layer was non-pigmented and culumnar with eosinophilic cytoplasm. Deep layer of epithelium was non-pigmented and cuboid with clear cytoplasm. There was not any pigments in subepithelial tissue. The subciliary process’s connective tissue had smooth muscle with longitudinal sections. There are a lot of thick elastic fibers in the ciliary body tissue. In conclusion, the major distinction of guinea pig in addition to differences of the lens and ciliary body’s dimensions was lack of pigmentation of epithelial cells of ciliary body.
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Ten quinea pigs with 400-900 g weight were used in this study. After removal of cranium bones from brain, they were assessed from an anatomical point of view (biometry, appearance, weight, ventricles) and also at a histological framework with H&E, PAS and malory sta More
Ten quinea pigs with 400-900 g weight were used in this study. After removal of cranium bones from brain, they were assessed from an anatomical point of view (biometry, appearance, weight, ventricles) and also at a histological framework with H&E, PAS and malory stainings. The results showed that the average length of cerebrum was equal to 2.41±0.048, the width of that at the thickest portion was equal to 1.21±0.027 and at the thinnest portion was 0.68±0.0034 cm. Also the thickness of cerebrum at the thickest and the thinnest was respectively equal to 1.21±0.023 and 0.72±0.0075 cm.The color of cerebrum was light redish. Gyri ans sulcus were absent. Cerebrum had a tip pointedshape like the tip of a pen. Pia mater with its related vasculature covers the surface of the brain. The preform lobes are very large. The average weight of cerebrum was equal to 3.4 g.. The lateral ventricle I and II were longe in caudal portion and in cranial have been directed downward with a severe deviation. The III &IV ventricles were wide, while sylvius duct is small. Histologically the pia mater is highly vascular and collagen fibers were detectable in it.The density of neurons at the molecular layer of the gray mater is low. This layer is completely differentiable from its down layers. External and internal granular layers were apparent, but the externalpyramidal layer is not obviously to the view. The internal pyramidal cells were represented very large. The depth of the grey mater had big cells named to betz cells.
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