Histophysiological and Histochemical Study of the Uterus, Cervix and Vagina in Guinea Pig (Cavia porcellus )

Subject Areas :

tahereh Mahmoudian ¹

1 - Animal physiology.Natural Science Faculty,University of Tabriz. Iran

Received: 2020-10-21 Accepted : 2021-05-20 Published : 2021-06-22

Keywords: Cervix, Vagina, Guinea pig, Keywords: Histophysiology, Uterus,

Abstract :

Inroduction & Objective: The importance of histophysiological and histochemical study of the uterus, cervix and vagina in mammals is due to pathological examinations of these organs in infectious and metabolic diseases and infertility treatment. The aim of this study was to investigate the histophysiological and histochemical features of these organs in guinea‌pigs.Materials and Methods: Five female guinea pigs with mean weight were obtained from laboratory animal breeding center and after sampling, Samples were stained by Hematoxin - Eosin, Periodic acid Schiff and alcian blue methods and then histological and histochemical properties of samples were samples were studied by light microscope.Results: The horn and the body of the uterus in general have three layers of endometrium , myometrium and primetrium. The endometrical epithelium in the uterine horn consists of simple cubic cells and it consists of simple cubic cells and simple columnar cells in the body of uterus. The endometrium has many coiled tubular glands. In the body and horn, the myometrium has 2 layers of longi tudinal and circular smooth muscle from outside to inside. The cervical mucosa has simple columnar cells. And has 3 types of folds: primary, Secondary and tertiary folds. Among the columnal cells, a large number of mucus secreting goblet cells are seen. The squomous vaginal epithelium is stratified stratum Corneum. no glands were observed in mucosal layer of the vagina.Conclusion: The results show that the anatomical a tissue structure of Guinea pig uterus, cervix and vagina, despite minor differences, are very similar to other mammals.s.

References:

1-آروند، م.1379. بافت شناسی عملی. چاپ اول. انتشارات دانشگاه علوم پزشکی و خدمات درمانی، مشهد، ص 350 - 354.

2-بانان خجسته، م.1391. آناتومی و فیزیولوژی کلینیکی مهره داران. چاپ اول. انتشارات پریور، تبریز، 1391؛ ص 247 - 248.

4-حائری روحانی، ع.، نامور، س.، صفری، ف.، شهابی، پ.1396. فیزیولوژی برن و لوی. چاپ چهارم. انتشارات اندیشه رفیع، تهران، ص880 - 897 .

5-دزفولیان، ع.، شریعت زاده، م.1386. بافت شناسی. چاپ اول. انتشارات آییژ، تهران، ص 219-222.

6-مهدوی شهری، ن.، فاضل، ع.، ژیان طبسی، م.، سعادت فر، ز.1380. تکنیک های هیستولوژی و هیستوشیمی. چاپ اول. انتشارات دانشگاه فردوسی، مشهد، ص364 - 376.

7-هاشمی، م.، حسنی، س.1380. فزیولوژی تولیدمثل. چاپ سوم. انتشارات فرهنگ جامع، تهران، ص 83 - 88.

8-یوسفی، الف.1368. بافت شناسی مقایسه ای و هیستوتکنیک. چاپ اول. انتشارات دانشگاه تهران، ص 287 -290.

9.Abood, DA., Al-Saffar, FJ. (2015). The post hatching development of the female genital system in indigenous Mallard Duck (Anas platyrhynchos). Iraqi J Vet Med., 39(2); 17–25.

10.Al-Saffar, FJ., Hazim, N.H., Al-Ebbad, I. (2019). Histomorphological and histochemical study of the uterus of the adult guinea pigs (Cavica porcellus). Indian Journal Science and Technology, 12(46); 10-17485.

11.Aughey, E. (2001). Comparative veterinary histology whit clinical correlates. 1nd ed. Grafos AS, Spain, 189-193.

12.Benbia, S1., Yahia, M1., Boutelis, S1., Chennaf, A1., Yahia, M. (2013). Evaluation of the cytology and histology of uterus and cervix as predictors of estrous stages in ewes and dairy cows. American Dairy Science Association, 5(6); 2437-2342.

13.Brezile, J. E., Brown, E. M. (1999).The biology of the guinea pig. New York: Academic Press. PP. 53-62.

14.Camila, CD., Patricia, FFP., Tania, MS., Carls, MM., Francisco, EM. (2001). Estrous cycle anatomy and histology of the uterine tube of the Mongolian gerbil (Meriones unguiculatus). Rev Chil Anat, 19; 191–6.

15.Chanut, FJA., Williams, AM. (2016). The syrian golden hamster estrous cycle: unique characteristics, visual guide to staging, and comparison with the rat. Toxicol Pathol., 44(1); 43–50.

16.Cooper, G., Schiller, A L. (1975). Anatomy of the guinea pig. Cambridge, Mass: Harvard University Press., PP. 17-71.

17.Cramer, M J. (2011). The biology of small mammals. 1nd ed. The American Midland Naturalist, University Of Noter Dome, 165(1); 204-205.

18.Espejel, MC., Medrano, A. (2017). Histological cyclic endometrial changes in dairy cows. An Overview, 25(5); 257-3.

19.Gdale, B. (1966). Reproduction in the ferret (Mustela furo). Uterine histology and histochemistry during pregnancy and pseudopregnancy . American Journal of Anatomy, 23(6); 2387-2367.

20.John, P., Georgene, H. (2012). Studies on the reproductive activities of the guinea pig: IV. A Comparison of Sex Drive in Males and Females, 45(2); 312-5.

21.Kobayashi, A., Behringer, RR. (2003). Developmental genetics of  the female reproductive tract in mammals. . Nature Reviews Genetics Journal, (5); 10-1038.

22.Kunzl, C., Sachser, N. (1999). The behavioural endocrinology of domestication: a comparison between the domestic guinea pig (Cavia aperea F. porcellus) and its wild ancestor, the cavy (Cavia aperea). Horm Behav, 35; 28–37.

23.Lindeberg, H. (2008). Reproduction of the female ferret (Mustela putorius furo). National Library of Medicine., 81(14); 1247-1256.

 

24.Rush, C. M., Hafner, L. M., Timms, P. (1996). The vaginal microbiota of Guinea pigs. Microbial Ecology in Health and Disease, 9; 123-127.

25.Ramachandraiah, SV., Narsimha, R.P., Ramamohana, Rao. (1980). Histological and histochemical changes in the uterine and oviductal epithelium of ewe during oestrous cycle. Indian Journal of Animal Sciences, 50; 41-45.

26.Steinhauer, N., Boos, A., Günzel-Apel, AR. (2004). Morphological changes and proliferative activity in the oviductal epithelium during hormonally defined stages of the oestrous cycle in the bitch. Reprod Domest Anim, 39(2); 110–19.