Pathologic study and time of persistence foot-and-mouth disease in guinea pig organs

Subject Areas : Journal of Comparative Pathobiology

ایرج سهرابی حقدوست ¹ , محمدحسن حبل الورید ² , همایون مهروانی ³ , محمد اسلام پناه ⁴ , حسن ایزدی ⁵

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Received: 2011-04-22 Accepted : 2011-08-23 Published : 2011-11-22

Keywords: Foot-and-mouth-disease, Guinea pig, Pathologic lesion, Elisa assay,

Abstract :

study on period of persistence of foot-and-mouth disease virus in different organs in guinea pigs .After adaptation of foot-and-mouth disease viruses type O in 10 guinea pigs pad’s, 30 Animaldivided to 5 groups containing 6 animals in each group so that 5 animals inoculated from eachgroup and normal saline injected in a guinea pig as negative control.Euthanized animal in 5 periods including: 2 days post virus generalization٫ 4 days post virusgeneralization ٫14 days post virus generalization٫ 30 days post virus generalization٫ 60 days postvirus generalization. Then collecting samples from: heart٫ lung٫ liver, spleen٫ pancreas٫retropharyngeal lymph node, palntar epithelium, inguinal lymph node and tongue Epithelium, sentto Pathology Department to pathological studies also other of those sent to FMD Department to viraldetection. Pathological samples fixed in formalin then prepared paraffin ،s blocks and slides with5 microns with H&E staining. Viral samples kept in glycerinated buffer and detected the amount ofvirus by Sandwich Elisa according to reference. At least base on our study the maximum of FMDVlesions we observed affected plantar Epithelium٫ Lung٫ Tongue Epithelium and spleen consequentlyand showed that pathologic lesion contained: Intracellular spongiosis, vacuolization and pyknosis ofcells from the spinous layer, Intercellular edema in spinous layer of Epithelium (plantar and Tongue)also Hyperemia and increased size of inter alveolar septa in Lung and decreased in white pulpcompared to uninfected control guinea pig in spleen . In addition results of Elisa determined extremeamount of virus were in 14 days post virus generalization and we have not any evidence of beingvirus in 30 and 60 days post virus generalization. It is concluded that the results of Pathologicalobservations can be confirmed by Elisa detection.