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Open Access Article
1 - بررسی شیوع سرمی IgG علیه توکسوپلاسما گوندی در طیور شهرستان سمنان، ایران
صادق حسینی مریم رسولی حمید استاجی -
Open Access Article
2 - Study of the Seasonal and Geographical Prevalence of Toxoplasma gondii in milk of ruminants by nested-PCR
M. Alipour Amroabadi E. Rahimi A. ShakerianToxoplasmosis is one of the most dangerous common protozoan diseases between humans and animals that infect humans in various ways. Contact with the infected animal, consuming contaminated food, and transmitting from mother to fetus is the most important means of transm MoreToxoplasmosis is one of the most dangerous common protozoan diseases between humans and animals that infect humans in various ways. Contact with the infected animal, consuming contaminated food, and transmitting from mother to fetus is the most important means of transmission. Based on the recorded data, this study is the first report of Toxoplasma gondii in buffalo and camel milk in some parts of Iran. For this purpose, 440 raw milk samples of 5 species of animals were collected from Isfahan, Chaharmahal & Bakhtiari, Khuzestan, and Fars provinces during four seasons and studied for the presence of Toxoplasma gondii by nested-PCR. The results showed that 26 (5.9%) of the samples were infected with Toxoplasma gondii. The highest infection was observed in sheep milk (8%) and the lowest in buffalo milk (4.28%). The seasonal prevalence of infection was in winter (11.43%), autumn (6.8%), and spring (5.03%), while none of the samples obtained in summer the parasite was not infected. Contamination of raw milk and traditional dairy products with Toxoplasma gondii can either occur directly from infected animals or as a result of non-compliance with the principles of hygiene during the process of milking, transportation, storage, and cross-contamination. Therefore, proper pre-consumption of milk is recommended. TRANSLATE with x English Arabic Hebrew Polish Bulgarian Hindi Portuguese Catalan Hmong Daw Romanian Chinese Simplified Hungarian Russian Chinese Traditional Indonesian Slovak Czech Italian Slovenian Danish Japanese Spanish Dutch Klingon Swedish English Korean Thai Estonian Latvian Turkish Finnish Lithuanian Ukrainian French Malay Urdu German Maltese Vietnamese Greek Norwegian Welsh Haitian Creole Persian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster Portal Back //TRANSLATE with xEnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW BackEMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack// Manuscript profile -
Open Access Article
3 - PCR assays for detection of Toxoplasma gondii infection in Iranian camels (Camelus dromedarius) of Yazd province
alireza sazmand mousa tavassoli bijan Esameilnejad zahra Asadollahi ali Kazemnia seyyedhossein Hekmatimoghaddam The protozoan parasite Toxoplasma gondii is one the most important zoonotic pathogens. Therefore, in the current pilot study a total of 50 Iranian one-humped camels of both sexes and different ages that were kept by local farmers in Yazd province of Iran we More The protozoan parasite Toxoplasma gondii is one the most important zoonotic pathogens. Therefore, in the current pilot study a total of 50 Iranian one-humped camels of both sexes and different ages that were kept by local farmers in Yazd province of Iran were tested for Toxoplasma gondii infection. Whole blood samples were investigated by PCR assay using B1 gene. The results revealed that none of the tested camels were infected with Toxoplasma gondii. The current pilot study is the first attempt for detection of Toxoplasma gondii in camels in Iran by PCR method. Further studies in different regions of the country seem necessary to outline the importance of the disease. Also, experimental infection of camels with Toxoplasma gondii is recommended to study the course of the disease. Manuscript profile -
Open Access Article
4 - Molecular Modeling of the Toxoplasma Gondii Adenosine Kinase Inhibitors
Taraneh Zandi AsadabadiComputer-assisted approaches might be seen as a bridge to novel medication discoveries. In 2014, the World Health Organization declared antibiotic resistance in microorganisms as a major global threat where simple diseases that were formerly manageable have now become d MoreComputer-assisted approaches might be seen as a bridge to novel medication discoveries. In 2014, the World Health Organization declared antibiotic resistance in microorganisms as a major global threat where simple diseases that were formerly manageable have now become deadly infections. Microbial resistance is a form of drug resistance where a microorganism may live even in the presence of antibiotics. Toxoplasmosis is a major worldwide parasitic infection caused by Toxoplasma gondii. Since Toxoplasma gondii is not capable of purine synthesis, the protein adenosine kinase (EC.2.7.1.20) is an important enzyme in its life pathway. Therefore, Toxoplasma gondii adenosine kinase has recently been considered a target for developing anti-Toxoplasma agents. This study aimed to develop a 3D QSAR model to predict the activity of adenosine kinase inhibitors in Toxoplasma gondii and to find new potent inhibitors. The acceptable values of 0.98, 0.83, and 0.91 were observed for the goodness of fit (R2), internal cross-validation (Q2), and external cross-validation (R2pred) indices, respectively. The robustness of the model was confirmed by applying the Y-scrambling analysis, and values of ~ 0.18 and ~ 0.0025 were observed for the R2 intercept and Q2 intercept, respectively. This confirmed that indices calculated for the original model were not based on the chance correlation between independent and dependent variables. New ligands were then proposed based on the structural virtual screening using the SwissSimilarity web tool and the ZINC database. The SwissADME web tool was used to predict the pharmacokinetic properties of the new compounds, and a promising compound was suggested for further research. Manuscript profile -
Open Access Article
5 - Evaluation of the immunogenicity of Toxoplasma gondii attenuated strain in cats
Pejman Houshmand Ahad Olyaei Darya Davoodi seyedeh zahra bootorabi Elham Ghazanfari Masoumeh Hayati Zahra Khabazan Fatemeh Dabiri Mohammad mehdi Namavari Fatemeh KhosraviBackground & Objectives: Toxoplasma gondii is a parasite which definitive hosts, Felid and cats, play a critical role in transmission and distribution of the parasite and their immunization is a proper way to control the infection. The aim of the present study is to MoreBackground & Objectives: Toxoplasma gondii is a parasite which definitive hosts, Felid and cats, play a critical role in transmission and distribution of the parasite and their immunization is a proper way to control the infection. The aim of the present study is to evaluate the immunogenicity of Toxoplasma gondii attenuated isolate produced in Razi Institute branch of Shiraz in cats Materials & Methods: Twelve male DSH (Domestic short hair) kittens aged 3-6 months were randomly divided into three groups; each contains four cats. The First group received tachyzoites of Toxoplasma gondii attenuated isolate at a dose of 10×106 by injection, the second group received a dose of 25×106 orally and the last group received culture medium as a control. The humoral immune responses and Cellular immunity evaluated by modified agglutination and skin test. One month after immunization, all cats were challenged by Toxoplasma's PRU strain, after which the cats' feces were collected and molecular tests were performed for five days. Results: The results of modified agglutination and skin test showed that administration of oral and injection vaccine had a significant increase in comparison with control. Also, Molecular testing after challenge revealed that immunization of cats by oral and injection way of Toxoplasma gondii attenuated isolate could successfully prohibit oocysts shedding in cats. Conclusion: In order to the significant immune responses and prevention of oocysts shedding, it is suggested that, using this isolation of Toxoplasma gondii as a possible candidate vaccine for cats in future studies. Manuscript profile -
Open Access Article
6 - مطالعه سرولوژیک توکسوپلاسموز در اسبان جنوب شرق ایران
شیوا امان الهی احسان اله سخائی مهدی گلچینتوکسوپلاسموز یک بیماری مشترک مهم محسوب شده که توسط توکسوپلاسما گونده ایجاد می شود. عامل بیماری از طریق بلع کیست های بافتی موجود در گوشت خام یا از طریق اووسیست های اسپروله شده در موادغذایی، خاک و آب انتقال می یابد. هدف مطالعه ی حاضر ردیابی پادتن ضد توکسوپلاسما گوند Moreتوکسوپلاسموز یک بیماری مشترک مهم محسوب شده که توسط توکسوپلاسما گونده ایجاد می شود. عامل بیماری از طریق بلع کیست های بافتی موجود در گوشت خام یا از طریق اووسیست های اسپروله شده در موادغذایی، خاک و آب انتقال می یابد. هدف مطالعه ی حاضر ردیابی پادتن ضد توکسوپلاسما گونده ای به روش لاتکس آگلوتیناسیون در اسبان استان های کرمان و یزد می باشد. به این منظور تعداد 163 نمونه سرم اسب (از نظر ظاهری سالم) از باشگاه های سوارکاری استان های مذکور اخذ و به روش لاتکس آگلوتیناسیون مورد ارزیابی قرار گرفتند. بر اساس نتایج حاصله تعداد 71 سرم از مجموع 163 نمونه مورد آزمایش (56/43 درصد) حاوی پادتن ضد توکسوپلاسما گونده ای بود. بنابراین به نظر می رسد که پادتن ضد عامل مذکور در اسبان استان های مورد نظر حضور داشته باشد. Manuscript profile -
Open Access Article
7 - The Relationship between ABO and Rhesus Blood Groups with Toxoplasmosis in Thi-Qar Province, Iraq
Sukaina Neamah Younus Abdullah -
Open Access Article
8 - Detection of B1 Gene of Toxoplasma gondii infection in Slaughtered Sheep in Isfahan Using the Nested PCR
Hamid Raieszadeh Abdolah Jamshidi Vahid Noaman Gholamreza RazmiToxoplasma gondii is a common pathogen in humans and animals which can infect humans through the food sources. The consumption of mutton is more popular than other sources of animal protein in Iran. Since the sheep heart is usually eaten half-cooked and grilled, the tra MoreToxoplasma gondii is a common pathogen in humans and animals which can infect humans through the food sources. The consumption of mutton is more popular than other sources of animal protein in Iran. Since the sheep heart is usually eaten half-cooked and grilled, the transmission of toxoplasmosis occurs more often through the consumption of sheep heart. On the other hand, because the density of parasitic cysts in the brain and heart tissue is higher than other oral organs, sheep heart samples were used in this study. In the recent study, to detect toxoplasma in sheep heart slaughtered in Isfahan, 250 heart samples were collected from different sexes and ages in different seasons over the course of one year. After extracting the parasite genome, two pairs of primers related to B1 gene were amplified by nested PCR method. The results showed that 78% of the samples were generally infected with toxoplasma. In this study, there was no significant relationship between infection percentage and age and sex, but infection rate in cold seasons was significantly higher than in the warm seasons. Since the factors such as high humidity and low-temperature help to oocysts' survival, long exposure with toxoplasma oocysts can increase the sheep infection rate in the cold season. By considering the importance of this disease in sheep deaths and abortions and the importance of public health, appropriate control programs to prevent this zoonotic disease at the provincial level should be included in veterinary policies. Manuscript profile -
Open Access Article
9 - Serological survey of Toxoplasma gondii in female Markhoz goats of Saqqez district, Kurdistan
Ghazaaleh Adhami Aram ParviziBackground & Purpose: Toxoplasma gondii is a meat-borne zoonotic parasitic protozoan that causes congenital infection, miscarriage, and stillbirth in the human and animals. Goat is an important resource of meat production in Kurdistan Province, and the consumption o MoreBackground & Purpose: Toxoplasma gondii is a meat-borne zoonotic parasitic protozoan that causes congenital infection, miscarriage, and stillbirth in the human and animals. Goat is an important resource of meat production in Kurdistan Province, and the consumption of its raw or half-cooked meat is a major risk factor for infection with Toxoplasma. The present study aimed to investigate the seroprevalence of Toxoplasma gondii infection in Markhoz goats of Saqqez City of Kurdistan province in western Iran.Materials & Methods: In a cross-sectional study, we utilized 188 serum specimens ( 144 serum samples belonging to goats with a history of abortion and 44 samples belonging to goats without a history of abortion ) for the evaluation of Toxoplasma gondii antibodies by ELISA. 38 samples out of 188 serum specimens in the study were positive for Toxoplasma gondii using ELISA, and the infection rate of the goats was 20.2%.Results: Two (3.57%) out of 56 Markhoz goats under 2 years of age were positive for Toxoplasma infection and 36 (27.27%) out of 132 Markhoz goats over two years of age were positive for the infection. The statistical examination indicated significant relationships between the pollution and age groups (p<0.05). 36 (25%) out of 144 Markhoz goats with a history of abortion were positive for Toxoplasma infection, and 2 (4.54%) out of 44 goats without a history of abortion were positive for the infection. The statistical studies indicated a significant correlation between the rates of infection and abortion (p<0.05).Conclusion: Given the prevalence of Toxoplasma gondii in Markhoz goats, we suggest providing information about prevention and control, refraining from eating uncooked or undercooked meat, and performing molecular studies. Manuscript profile
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