Investigation of the frequency of Toxoplasma infection in sheep of Alborz province by nested PCR method

Seyed Reza Hosseini ¹ ( استادیار، گروه آموزشی انگل شناسی،دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی،شهرکرد، ایران )
نادیا طایفی ² ( گروه پاتو بیولوژی دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد کرج )
Milad Hamzehali Tehrani ³ ( گروه پاتوبیولوژی- دانشکده دامپزشکی- واحد علوم و تحقیقات تهران- دانشگاه آزاد اسلامی نهران )

Submited date : 2024-07-31 Accepted date : 2024-12-22

Keywords: sheep, Toxoplasma gondii, PCR, zoonosis. ,

Abstract :

Abstract Introduction: Today, due to the increasing consumption of sheep meat and the popularity of this animal's meat, as well as the zoonosis of Toxoplasma gondii disease between humans and animals, as well as the financial losses caused by this parasite to livestock farmers, attention should be paid. Purpose: Our purpose in this research is to evaluate the detection of Toxoplasma by nested PCR method in sheep blood it was in Karaj province. Research implementation method: In this research, after collecting test samples and recording the characteristics and information of each sample, the presence of Toxoplasma gondii parasite in the sheep of Karaj province was investigated using the nested PCR method. Then all the obtained data were analyzed with the help of SPSS software. Results: The obtained results show that 9 samples (11.1%) of the tested samples (81 samples) have a level of contamination with Toxoplasma gondii, also a significant relationship between the age and sex of the animal with contamination with Toxoplasma gondii has been seen. Discussion and conclusion: The results obtained in this report show the necessity of examining the safety of parasites in Iran, because most of Iran's livestock are raised in a traditional way and preventive care for parasitic contamination is less. It can be a potential risk for the health of animals and their meat consumers.

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