In order to investigate the biochemical changes in serum of sheep infested by gastrointestinal nematodes, biochemical elements such as calcium, phosphorus, Magnesium, Alkaline phosphates, Total protein, Albumin, Alpha globulins, Beta globulins and Gama globulins were me More
In order to investigate the biochemical changes in serum of sheep infested by gastrointestinal nematodes, biochemical elements such as calcium, phosphorus, Magnesium, Alkaline phosphates, Total protein, Albumin, Alpha globulins, Beta globulins and Gama globulins were measured andcompared with those of the control animals. The results were analyzed by one-way ANOVA and Duncan multiple rouge test. Statistically significant differences were observed in calcium and phosphorus of serum between infested and control groups (p<0.05). Total protein and Albumin were significantly lower in the infested group (p<0.5) and Alpha globulins were significantly higher in this group (p<0.005).Hence infestation to gastrointestinal nematodes in sheep causes distinct of changes in serum parameters.
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این پژوهش به منظور شناسایی دام­های دارای مقاومت ژنتیکی نسبت به نماتودهای دستگاه گوارش (GIN) با استفاده از چندشکلی­های ریزماهواره­ای موجود در ژن Ovar-DRB1 برههای نژاد قزل ایرانی طراحی شد. در پژوهش حاضر 120 بره نر قزل 4 تا 6 ماهه به صورت تصادفی از 6 گله گوسف More
این پژوهش به منظور شناسایی دام­های دارای مقاومت ژنتیکی نسبت به نماتودهای دستگاه گوارش (GIN) با استفاده از چندشکلی­های ریزماهواره­ای موجود در ژن Ovar-DRB1 برههای نژاد قزل ایرانی طراحی شد. در پژوهش حاضر 120 بره نر قزل 4 تا 6 ماهه به صورت تصادفی از 6 گله گوسفند متفاوت در استان آذربایجان شرقی انتخاب شدند (تعداد 20 گوسفند در هر گله). این برهها به صورت طبیعی با GINها آلوده شده و نمونههای مدفوعی هر حیوان نیز جهت شمارش تعداد تخم­انگل موجود در مدفوع (FEC) به صورت دوبار در هفته و با یک هفته فاصله جمعآوری شد. نمونههای خونی نیز جهت استخراج DNA جمعآوری شدند و PCR به منظور افزوده­سازی اگزون شماره ­2 و توالی­های ریزماهوارهای موجود در اینترون شماره­2 ژن Ovar-DRB1 انجام شد. دادهها با استفاده از رویه مختلط در نرم افزار SAS آنالیز شدند. در پژوهش حاضر 24 ژنوتیپ و 20 آلل برای ژن Ovar-DRB1 شناسایی شدند. نتایج نشان دادند که حضور آللی به طول 510 جفت ­باز (که آلل F نامیده شد) در هر دو دامهای هموزیگوت و هتروزیگوت دارای رابطه­ قابل­توجه معنی­داری (01/­0P<) با کاهش FEC دارد، در حالیکه حضور آللی به طول 506 جفت­باز (که آلل E نامیده شد) در دام­های هموزیگوت نیز دارای رابطه معنی­داری (01/­0P<) با افزایش میزان FECمی باشد. بنابراین این پژوهش رابطه قابل­توجه معنی­داری را بین چندشکلی­های ریزماهوارهای موجود در ژن Ovar-DRB1 و مقاومت به GIN در برههای نژاد قزل نشان داد.
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The cereal cyst nematodes are one of the most important soil-born pathogens on wheat and barley throughout the world. The two species of the cereal cyst nematodes, H. filipjevi and H. latiponsare widely distributed in Iran, but H. avenae has limited distribution. A fiel More
The cereal cyst nematodes are one of the most important soil-born pathogens on wheat and barley throughout the world. The two species of the cereal cyst nematodes, H. filipjevi and H. latiponsare widely distributed in Iran, but H. avenae has limited distribution. A field survey was conducted for determining of distribution regions and population density of the cereal cyst nematodesin Markazi province in 2009. In this study 83 soil sample of wheat and barley fields were taken from Arak, Shazand, Farmahin, Ashtian, Komijan, Tafresh, Zarandieh, Mahallat, Khomein and Saveh regions. The modified Fenwick can technique was used to extract cysts from the soil. Based on the morphological characteristics of the second stage juveniles and the vulval cone structures, two species were identified as H. filipjevi and H. latipons. The results showed that H. filipjevi was the most dominant species in both wheat and barley fields, whereas, H. latipons was found on wheat in Khomein and Zarandieh regions. H. filipjevi had more distribution and population density in Farmahin, Tafresh and Khomein regions in comparison to other regions. The cereal cyst nematodes were found in 39.7% of rain-fed fields of wheat and barley of the Markazi province. The most of nematode population density was determined (20-40 cyst per 300 g of soil) in some cereal fields that can be as one of limiting factor in yield reduction.
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Background: There are many zoonotic diseases between humans and animals that can be a threat to human health. These include gastrointestinal parasites in pets such as dogs and cats.Objectives: Because of the temperate climate in Tabriz city, severe parasitic infections More
Background: There are many zoonotic diseases between humans and animals that can be a threat to human health. These include gastrointestinal parasites in pets such as dogs and cats.Objectives: Because of the temperate climate in Tabriz city, severe parasitic infections have been reported in animals of the region. These parasitic infections need further epidemiological study and identification of different species, to eradicate various parasitic diseases.Methods: July 2016 to March 2017, 63 stray dogs and 63 domestic dogs from Tabriz city were sampled. Then, using the flotation method, eggs and parasite cysts were identified in infected dogs.Results: By examining the eggs of parasites that were isolated from the feces of stray and domestic dogs, Toxocara in stray dogs with 49.20% and hookworms in domestic dogs with 14.28% had the highest rate of infection, and the lowest rate of infection in stray dogs was related to Cryptosporidium with 7.93%.Conclusions: According to this study, a lower parasitic infection rate was observed in the gastrointestinal tract of domestic dogs. These results refer to the importance of an appropriate living environment, healthy nutrition, and an anti-parasite treatment program. Attention to these issues in the clinic leads the veterinarian to the correct diagnosis.
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The purpose of this project is to investigate the genetic resistance of Ghezel and Ghezel-Romanov lamb to gastrointestinal parasites in East Azerbaijan province. For this study, were used 10 male and female Ghezel lambs and 10 male and female Ghezel-Romanov lambs. The a More
The purpose of this project is to investigate the genetic resistance of Ghezel and Ghezel-Romanov lamb to gastrointestinal parasites in East Azerbaijan province. For this study, were used 10 male and female Ghezel lambs and 10 male and female Ghezel-Romanov lambs. The anti parasitic drug was administered on day of the test and then 2 weeks later. The studied traits included: Famacha test, hematocrit test, fecal score and number of nematode eggs per gram of feces. Data were analyzed using SAS software in a completely randomized design. The results showed that the Famacha test was significant in male and female Ghezel and Ghezel-Romanov mixed breeds (p<0.05). number of nematode eggs in male and female Ghezel-Romanov lambs had a significant decrease compared to Ghezel lambs (p<0.05). number of parasite eggs in Ghezel-Romanov lambs was lower than Ghezel breed, in which male Ghezel-Romanov lambs had the lowest number of parasite eggs (16.2) (p<0.05). Crossbreeding of Ghezel and Romanov increases the relative resistance to gastrointestinal nematodes in terms of traits measured in this study.
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