Meat products

Open Access Article

1 - Histological and microbial analysis of different types of raw meat products in Tehran, Iran
Zohreh Mashak Zahra Mahdizadeh Barzoki Amirreza Saadati Maziar Taghavi

10.30495/fh.2023.73971.1141

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Open Access Article

2 - Incorporation of essential oils (EOs) and nanoparticles (NPs) into active packaging systems in meat and meat products: A review
Mina Kargozari Hassan Hamedi

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Open Access Article

3 - Evaluation of Presence of Soya Protein in Some Commercial Meat Products by Polymerase Chain Reaction Assays
sara Bazyari Hamid Reza Zamani Zadeh Maryam Mizani Anoshe Sharifan

Introduction: Soya is used in meat products due to its functional and nutritional properties. Soya is also known as an intense allergen substance for some people according to the Institute of Standards and Industrial Research of Iran, therefore its presence must be ment More

Introduction: Soya is used in meat products due to its functional and nutritional properties. Soya is also known as an intense allergen substance for some people according to the Institute of Standards and Industrial Research of Iran, therefore its presence must be mentioned on the label of the products. The purpose of this study is to develop a PCR assay for identifying soy protein as a hidden allergen substance in meat products. Materials and Methods: In this study, soya and meat products samples were prepared. The extraction of DNA from samples were carried out according to the instruction provided by MBST kit. The specific primer pairs of soya were designed and ordered. The PCR conditions were standardized to obtain the desired amplicon of 118bp for soya. Finally the PCR products were electrophoresed in 1.5% agarose gels. This method was used for detection of soya in commercial meat products and the results were compared with their labeling statements. Results: In optimized PCR condition, DNA fragment of 118bp for soya was amplified. This developed technique was successfully evaluated for detection of soya in meat products. The results showed that 100% of sausages and chicken nuggets, 33.3% of cold cuts, 60% of hamburgers and 75% of Loghme Kebab contained soya. Conclusion: The results indicated that PCR assay developed in this study was a rapid, sensitive, simple, reliable and commodious method that might be used for the detection of soya in raw and heat treated meat products. Manuscript profile

Open Access Article

4 - Detection Of Enterotoxigenic Bacillus cereus Isolated from Meat Products from Zanjan
Zahra Deilami Khiabani GHazaleh Shamsolmaali

Inroduction and Objective:Most of Bacillus cereus isolates can cause diarrhoeal and emetic types of food poisoning. The diarrhoeal illnesses can be caused by hemolysin BL (HBL), non-hemolytic (NHE) and cytotoxin K. Use of meat products is common nowadays. There is no pr More

Inroduction and Objective:Most of Bacillus cereus isolates can cause diarrhoeal and emetic types of food poisoning. The diarrhoeal illnesses can be caused by hemolysin BL (HBL), non-hemolytic (NHE) and cytotoxin K. Use of meat products is common nowadays. There is no precise report ofB. cereus contamination in Iran, therefore it is important to study in this case.Materials andMethods:80 meat products were collected form stores in Zanjan then the samples were cultured in PEMPA. Following the biochemical tests, the bacterial colonies were identified by PCR. Then B. cereus isolates were checked for NHE complex genes by specific primers using PCR.Results:Results showed that 32 samples of 80 were contaminated with B. cereus. The NHE complex genes were found in 14 samples.The results of this study have shown that NHE PCR is a prompt, reliable method for differentiation between non-enterotoxigenic and enterotoxigenic isolates of B. cereus. Conclusion:Enhancing awareness about virulence and prevalence of genes involved in food poisoning would be effective in the prevention of food poisoning Manuscript profile

Open Access Article

5 - Evaluation of nitrite residue in Sausages, marketed in Tabas city
Mohammad Reza Marvimoghadam Ataollah Azhdari Gholam Reza Sharifzadeh

10.30495/qafj.2022.1971707.1045

Nitrite and nitrate are used in some products, such as sausages. Excessive consumption of these chemicals is hazardous for humans, so the use of these compounds must be within the standards ranges. The aim of this research was measurement of nitrite residue in meat prod More

Nitrite and nitrate are used in some products, such as sausages. Excessive consumption of these chemicals is hazardous for humans, so the use of these compounds must be within the standards ranges. The aim of this research was measurement of nitrite residue in meat products which were marketed in Tabas city. Totally100samples from four different meat products were collected randomly and studied in the food laboratory of South Khorasan provincial of standard and industrial research service by spectrophotometric method. Data were analyzed with SPSS-19 software. The statistical analysis of data showed that the nitrite content in Mortadella, dry sausages, Bockwursts and cocktail sausages was higher than Iranian standard limit in 24%, 16%, 12% and 4% of samples, respectively and in this regard, 14% of all samples were distinguished unusable. The average amount of nitrite in all samples was 72.33± 2.8ppm, moreover the highest and the lowest level of nitrite observed in Mortadella (92±4.7 ppm) and Cocktail sausages (52.1±3.04 ppm), respectively, also the average amount of nitrite in all samples and different brands, was lower than the Iranian standard limit, significantly (P<0.05). The results of this survey indicate that nitrite residue in 20% of Mortadella & dry sausage samples and 8% of Bockwursts & cocktail sausage samples were higher than the standard limit, so it is believed that stricter preventive measuring is necessary and partial replacement of nitrate with natural preservatives is also recommended Manuscript profile

Open Access Article

6 - Determination of oxytetracycline residues in the distributed meat products in Tabriz by high-performance liquid chromatography method
Jafar Shadjou Mohammad Hosein Movassagh

10.30495/jfh.2023.1989199.1405

The excessive use of antibiotics in animal husbandry has led to the appearance of antibiotic residues in meat and could threaten public health. The study aimed to determine the oxytetracycline residue level in meat products in Tabriz. A total of 60 samples of meat produ More

The excessive use of antibiotics in animal husbandry has led to the appearance of antibiotic residues in meat and could threaten public health. The study aimed to determine the oxytetracycline residue level in meat products in Tabriz. A total of 60 samples of meat products (sausages, Persian sausages and hamburgers, 20 samples from each meat product) were randomly collected from food supply stores in Tabriz from March to May 2021. High-performance liquid chromatography was used to determine the level of oxytetracycline residue levels in the samples. The results showed that the mean of oxytetracycline residue in sausage, Persian sausage, and hamburger was 82.48±7.06, 57.35±4.27, and 150.79±3.65 µg/Kg, respectively. The mean value of oxytetracycline residues showed a significant (p<0.05) difference between the three products. Also, oxytetracycline residue was observed in all samples. However, in all samples, it was below the allowed limit of Codex Alimentarius (200 µg/kg). The average residue of oxytetracycline in hamburgers was significantly higher than in sausages. In hamburgers with 90% meat, the average amount of oxytetracycline was more than in hamburgers with 70% meat, which can be related to the percentage of meat in the product (p<0.05). Although the level of oxytetracycline residue in the present study was less than the permissible limit, considering the consumption of meat products in the country, it is necessary to control and monitor the presence of antibiotics in foods of animal origin. Manuscript profile

Open Access Article

7 - Survey of unauthorized tissues in meat products by histological method in Tabriz and Khoy
Ayda Payamei Mohammad Hosein Movassagh Masoud Delashoub

10.30495/jfh.2021.1935193.1320

Nowadays, the consumption of meat and meat products in different countries varies considerably based on social, economic, and geographical differences. Meat and meat products are the most important sources of protein. This study aimed to investigate the possibility of u More

Nowadays, the consumption of meat and meat products in different countries varies considerably based on social, economic, and geographical differences. Meat and meat products are the most important sources of protein. This study aimed to investigate the possibility of unauthorized tissues in sausage products in Tabriz and Khoy by histotechnique. For this, 80 sausages samples with ≤ 55% and > 55% 55% meat content were collected (from January to March of 2015). For histological evaluation, the slides were stained with Hematoxylin and Eosin. Based on results, in 87.5% of the samples, cartilage tissue with a high percentage was observed. Unauthorized tissues types such as glands, connective tissue, fat, skin, cartilage, bone, trachea, lung, bladder, parasite eggs, and intestine were observed. The presence of unauthorized tissues in most samples may reduce consumers' desire to use sausages. Consequently, there is a necessity for efficient control at the regional level by authority organizations. Manuscript profile

Open Access Article

8 - Study on the overview on food borne bacteria in foodstuffs with animal origin in Iran; Part two: meat and meat products
S.S Shekarforoush نوردهر Rokni گیتی Karim S.M Razavi Rohani S.M.M Kiaie مریم Abbasvali

   The present study was aimed to review the contamination of meat and meat products with pathogenic bacteria in Iran. Little information is available about the contamination of meat and meat products with Bacillus cereus, because of the low contamination rate More

   The present study was aimed to review the contamination of meat and meat products with pathogenic bacteria in Iran. Little information is available about the contamination of meat and meat products with Bacillus cereus, because of the low contamination rate with the microorganism. The situation is about the same with Brucella, as the microorganism can hardly be seen in the muscles. However, not too many information is available on the contamination with Campylobacter jejuni, even though, the contamination of chicken meat with the campylobacter was addressed. Clostridium butolinum was the main discriminated bacterium to contaminate fish and its products and canned meats. Studies revealed that the big portions of meat were less likely contaminated with E. coli compared to the minced meat. Our study showed that the cross contamination of sausage is often occurred after the production chain. The situation was possibly attributed to the improvement of hygienic conditions of slaughter-houses and meat industries. Limited information was found on the contamination with Listeria monocytogenes and available data indicate that the microorganism can be present in meat and meat products. The most important factor for prevention of contamination is restricted inspection of slaughtered animals before slaughter and omission of diseased animals. Much information was accessible on the cross contamination with Salmonella in Iran and elsewhere. Salmonellosis is not common in the slaughtered animals despite its epidemiological and public health issues. The problem was also associated with the restricted inspection. Study about the staphylococcal contamination of meat was proportionately numerous. The contamination was mostly occurred in the minced Kebab in the warm seasons of the year. Generally, a low percentage of such contaminations were found in the meat products and sausage, in the surveys. Manuscript profile

Open Access Article

9 - Isolation, identification and the presence of enterotoxin A gene in Staphylococcus aureus from meat products
مهسا Sepidarkish مسعود Ghane

   Staphylococcus aureus is one of the main causes of food-born illnesses. The aim of this study was to determine the prevalence of S. aureus in meat products and to detect the presence of S. aureus enterotoxin A (SEA) gene. Totally 150 meat products were coll More

   Staphylococcus aureus is one of the main causes of food-born illnesses. The aim of this study was to determine the prevalence of S. aureus in meat products and to detect the presence of S. aureus enterotoxin A (SEA) gene. Totally 150 meat products were collected and analyzed using standard culture techniques to detect S. aureus. PCR assay by specific primers was performed on the isolates to identify SEA gene. According to the results, 19 (12.6%) of the samples were found positive for S. aureus. Highest prevalence rate was determined in smoked fish (30%), followed by fried morsels (16.6%), Salami and Ham (13.3%), and Shensel chicken (3.3%). S. aureus was not observed in any of Sausage samples. Statistical analysis showed that there is statistically significance association between the prevalence of S. aureus and  meat products. Moreover, results did not show SEA gene in any of the isolates. This study concluded a remarkable occurrence of S. aureus in meat products.   Manuscript profile

Open Access Article

10 - Comparison of calorimetric and HPLC methods in measuring nitrate content of meat products: An economic evaluation
مسعود moradi کیومرث SHarafi سهراب Delangizan ستار Jamshidpoor

Nitrate is a flavors compound, color stabilizer and growth inhibitor of anaerobic microorganisms in meat products. High concentration of nitrate in food can cause methaemoglobinaemia and is recognized as carcinogenic. Therefore, accurate determination of nitrate is cruc More

Nitrate is a flavors compound, color stabilizer and growth inhibitor of anaerobic microorganisms in meat products. High concentration of nitrate in food can cause methaemoglobinaemia and is recognized as carcinogenic. Therefore, accurate determination of nitrate is crucial to ensure consumers’ health. Regularly, nitrate is estimated by colorimetric and HPLC methods. In measurement of nitrate, the efficiency, accuracy, speed and amount of material are important from economical point of view. In this study, the cost of initial investment, staff, consumable material and equipments used by the two methods were calculated and finally Net Present Value (NPV) was estimated for each of them. The rate of interest was considered from 4 until 30%. According to the results the amount of initial investment, annual cost of staff and consumable materials for colorimetric method were determined as 274000000, 379080000 and 214289130 Rials, respectively. These costs for HPLC method were 342000000, 252720000 and 7633080 Rials, respectively. NPV in minimum and maximum rates of interest (4 and 30%) for colorimetric method were 8368344000 and 2242330000 Rials and for HPLC method were estimated at 4035848000 and 1207544000 Rials. As a consequence, HPLC is more economical and could be recommended for the routine measurement of nitrate in of food safety laboratories. Manuscript profile

Open Access Article

11 - The Study of Effect of Natural Warm Pear Wood Smoke on Quantitative Characteristics of Cured Chicken Ham without Nitrite
Arthur Kalestians Ali Reza Shahab Lavasani Sara Movahed

The use of nitrite as preservative and color stabilizer in meat products like hams is common. Due to its carcinogenic effects, it has negative track record among consumers. On the other hand, low concentrations increases the risk of Clostridium botolinum growth during s More

The use of nitrite as preservative and color stabilizer in meat products like hams is common. Due to its carcinogenic effects, it has negative track record among consumers. On the other hand, low concentrations increases the risk of Clostridium botolinum growth during storage and the production of toxin of botulism. By replacing this preservative with warm and natural pear wood smoke, in suggested situations, a safe product without having carcinogenic affects can be produced.Therefore, the general objective of this study was to investigate the effect of hot and natural wood smoke on the quality of chicken ham without nitrite at 15, 30, 45 and 60 minutes. In this study, treatments were smoked by warm and natural pear wood for 15, 30, 45 and 60 minutes after production, vacuumed and kept for 50 days under refrigerate condition (1-4 C°) and were examined physicochemical, microbial and the amount of compounds Benzo-a-Pyrene in 10, 20, 30, 45, 50 days. The results showed that there was a significant difference between moisture content, protein, ash and control in other treatments (P≤0.05). There was a significant difference between the control and other treatments for microbial characteristics (Clostridium perfringens) (P≤0.05). And the residual amount of Benzo-a-Pyrene was evaluated in fumigated treatments within the standard range(<0.5µg/Kg). Relying on chemical and microbial results, treatments without nitrite and smoked for 45 and 60 minutes, were the best among other treatments in comparison with the control treatment. Manuscript profile

Open Access Article

12 - Prevalence of Staphylococcus aureusin meat and meat products
Fatemeh Nonahal Ebrahim Rahimi Esmaeil AtaieSalehi

Abstract Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made Staphylococcus has one of the most important factors causing the More

Abstract Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made Staphylococcus has one of the most important factors causing the food poisoning. This study was done to investigate the prevalence of Staphylococcus in meat and meat products in Esfahan. From summer to winter 2012, 450 samples including raw beef (n=80), minced meat (n=80), lamb (n=80), goat (n=80), camel (n=50), hamburger (n=40), kebab (n=40) were randomly collected from butcheries and supermarkets in Isfahan, and analyzed for the presence of S. aureus. Totally, 234 (55/6 %) S. aureus were detected in meat and meat products. The mean count of S. aureus in positive samples was 8/3×102. The results indicated that although the percentage of contaminated samples was high, the number of bacteria presented in the samples is low with no potential risk for public health. To effectively manage the food production for decreasing diseases more epidemiologic investigations about S. aureustoxication are needed. Manuscript profile

Open Access Article

13 - Prevalence of Staphylococcus aureus in meat and meat products
Fatemeh Nonahal Ebrahim Rahimi Esmail Ataie Salehi

Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made  Staphylococcus has one of the most important factors causing the food More

Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made  Staphylococcus has one of the most important factors causing the food poisoning. This study was done to investigates the prevalence of Staphylococcus in meat and  meat products  in  Esfahan. From summer to winter 2012, 450 samples including  raw beef (n=80), minced meat (n=80), lamb (n=80), goat (n=80), camel (n=50), hamburger (n=40), kebab (n=40) were randomly collected from butcheries and supermarkets  in Isfahan, and analyzed for the presence of S. aureus. Totally, 234 (55/6 %) S. aureus were detected in meat and meat products. The mean count of S. aureus in positive samples was 8/3×102. The results indicated that although the percentage of contaminated  samples was high, The number of bacteria presented in the samples is low with no potential risk for public health. To effectively manage the food production for decreasing diseases more epidemiologic investigations about S. aureus toxication are needed. Manuscript profile

Open Access Article

14 - Review and Prioritization of Various Advertisement Methods by Meat Products Matrix Analytic Hierarchy Method (AHP): A Case Study of Sausages Products
Sara Esfandi Ahmad Fatahi

Advertising is not only as a tool to increase company sales. Fast development of mass communication tools and added new mediums to advertising media have been introduced the advertising as a key element in the success or failure of a company. Due to the intense competit More

Advertising is not only as a tool to increase company sales. Fast development of mass communication tools and added new mediums to advertising media have been introduced the advertising as a key element in the success or failure of a company. Due to the intense competition between companies and the fast growth of markets and changes in consumer behavior, advertising is taken into consideration as a major tool to create consumer awareness of products and services. Thus, in order to choose the best advertising method for meat products (sausages and salami), comments of 500 Shiraz citizens are collected by Cochran sampling and used in data analysis by Analytical Hierarchy Process (AHP). After gathering the data, by synthesis the comments of respondents through arithmetic mean, the pairwise comparison matrix of criteria and priorities was formed to estimate the relative weights of them to achieve the goals of "the best method of advertising". The results indicated with respect to citizen viewpoints, the media advertisement, the street billboards, the advertising brochures, the advertising kiosks, and the purchase awards have the highest degree of importance with weight 0.430, 0.238, 0.139, 0.098 and 0.096, respectively. Due to the competition of different meat products factories, to make interest and encourage for purchasing a goods toward the competitor goods, it is necessary to use the persuasive advertisement. Manuscript profile

Open Access Article

15 - Evaluation of Nitrite Exposure from Meat Products Supplied in Tehran, Iran
Parisa Sadighara Behrouz Akbari-adergani Enam Shokri Amir Tabaraki Sara Mohamadi Tayebeh Zeinali

10.22034/jchr.2022.1939656.1403

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