Detection Of Enterotoxigenic Bacillus cereus Isolated from Meat Products from Zanjan
Subject Areas :
Zahra Deilami Khiabani
1
(
دانشگاه آزاد اسلامی واحد زنجان، استادیار دانشکده علوم پایه و پزشکی، زنجان.ایران
)
GHazaleh Shamsolmaali
2
(
دانشگاه آزاد اسلامی واحد زنجان، دانشکده علوم پایه و پزشکی کارشناس ارشد گروه میکروبیولوژی، زنجان.ایران
)
Keywords: Meat products, Bacillus cereus, NHE complex, PCR,
Abstract :
Inroduction and Objective:Most of Bacillus cereus isolates can cause diarrhoeal and emetic types of food poisoning. The diarrhoeal illnesses can be caused by hemolysin BL (HBL), non-hemolytic (NHE) and cytotoxin K. Use of meat products is common nowadays. There is no precise report ofB. cereus contamination in Iran, therefore it is important to study in this case.Materials andMethods:80 meat products were collected form stores in Zanjan then the samples were cultured in PEMPA. Following the biochemical tests, the bacterial colonies were identified by PCR. Then B. cereus isolates were checked for NHE complex genes by specific primers using PCR.Results:Results showed that 32 samples of 80 were contaminated with B. cereus. The NHE complex genes were found in 14 samples.The results of this study have shown that NHE PCR is a prompt, reliable method for differentiation between non-enterotoxigenic and enterotoxigenic isolates of B. cereus. Conclusion:Enhancing awareness about virulence and prevalence of genes involved in food poisoning would be effective in the prevention of food poisoning
