List of Articles Hypericum Perforatum

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  1 - The Effect of Nitrogen and Zinc Levels on Essential Oil Yield and some Morphological Traits of Hypericum perforatums
  M.R. Zadeh Esfahlan A. Ebadi N. Farsad Akhtar
  To study the effects of nitrogen and zinc fertilizer on the morphological traits and essential oil yield of St. John’s wort (Hypericum perforatum) a greenhouse experiment in a factorial randomized complete block design with three replications was conducted at Univ More

  To study the effects of nitrogen and zinc fertilizer on the morphological traits and essential oil yield of St. John’s wort (Hypericum perforatum) a greenhouse experiment in a factorial randomized complete block design with three replications was conducted at University of Tabriz, Iran in 2012. Treatments consisted of three levels of zinc sulphate with a concentration of zinc fertilizer (zero, 3 and 6 parts per thousand) and four levels of nitrogen fertilizer (zero, 50, 100, 150 kg/ha). One half of the fertilizers were applied 20 days after planting of plants and the rest 40 days after transplanting. Traits evaluated were plant height, inflorescence number, leaf area, plant fresh and dry weights and plant essential oil content. The results showed that the traits under study were affected by rate of fertilizer applications. Highest plant height, number of inflorescences, leaf area and essential oil yield were obtained by using 150 kg/ha of nitrogen and applying zinc with 0.006 concentration. Highest fresh and dry weights of above ground parts were also produced by using 150 kg/ha of nitrogen fertilizer along with zinc fertilizer 0.003. Manuscript profile
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  2 - Identification of active components in the extracts of Hypericum Perforatum L. and Oliveria Decumbens Vent. and evaluation of their antioxidant and antimicrobial effects on some microorganisms
  Mahsa jafarpour Hajar Abbasi Mohammad Goli
  10.30495/jfh.2023.1972178.1380
  Essential oils and extracts of medicinal plants have been highly regarded as natural food additives and preservatives due to the presence of bioactive compounds and functional activities, including antioxidant and antimicrobial properties. In this research, Hypericum pe More

  Essential oils and extracts of medicinal plants have been highly regarded as natural food additives and preservatives due to the presence of bioactive compounds and functional activities, including antioxidant and antimicrobial properties. In this research, Hypericum perforatum and Oliveria decumbens were extracted by the maceration method and ultrasonic waves. Total phenolic compounds, and antioxidant as well as antimicrobial activities were assessed by GC-MS. Minimum inhibitory and minimum bactericidal concentrations against Escherichia coli, Staphylococcus aureus, and Aspergillus niger were evaluated. The results showed that 19 main compounds, the most important of which are Thymol (24.40%), Carvacrol (23.00%), and Myristicin (9.78%), comprise 90.22% of O. decumbens extract. In the extract of H. perforatum, 20 compounds constitute 94.16% of the extract, among which Hypericin (25.92%) and Hyperforin (16.69%) are the most important effective compounds. The content of phenolic compounds of Hypericum perforatum and O. decumbens extracts were 24.0 and 9.5 mg/g of dry weight, respectively. H. perforatum extract shows higher antioxidant activity compared to O. decumbens extract (IC50 of H. perforatum and O. decumbens extracts are 0.173 and 0.470 g/ml, respectively). The extract of H. perforatum inhibited the activity of S. aureus and E. coli, however, did not significantly inhibit the activity of A. niger. Manuscript profile
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  3 - Clinical evaluation of the effect of hydroalcoholic extract of Hypericum perforatum on the healing of oral lesions of Foot and mouth disease in cattle
  mohammad tolouei amir daneshvar hosein tayefi-nasrabadi omid madadgar
  10.30495/jvcp.2021.1923074.1299
  Formation of excessive blisters and sores on the mouth of affected cattle with Foot-and-Mouth disease and the insufficiency of existing therapeutic methods, leads to extensive damages to the affected herds. The extract of Hypericum perforatum and its active ingredient, More

  Formation of excessive blisters and sores on the mouth of affected cattle with Foot-and-Mouth disease and the insufficiency of existing therapeutic methods, leads to extensive damages to the affected herds. The extract of Hypericum perforatum and its active ingredient, Hypericin, have inhibitory effects on the adsorption ability of Foot-and-Mouth virus to host cells in vitro. The medicinal plant also has an effective role in healing various kinds of wounds. The aim of this study was to evaluate the efficacy of H. perforatum extract on the healing of FMD oral lesions in affected cattle. In this study, 72 affected cattle (36 heads in 3 treatment groups of 12 and 36 heads in 3 control groups of 12) from 3 conflict centers in West-Azarbaijan province were selected. Determination of serotype by complement fixation method indicated that the studied animals were infected with serotype O of FMD. Dry aerial parts of H. perforatum were extracted with maceration method and 0.25, 0.5 and 5% concentrations of hydroalcoholic extract were prepared. In control groups, normal saline solution and in treatment groups, the extract with the mentioned concentrations was used as an oral spray (twice a day for 4 days). Cattle treated with 5% extract had a significant improvement in oral lesions and lesion repair during the treatment period was significantly different from the control groups (p<0.05). The results showed that spraying 5% concentration of H. perforatum extract had significant healing effects on oral lesions caused by FMD. Manuscript profile
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  4 - Evaluation of flavonoids and phenolic compounds, antioxidant and antibacterial activity of Hypericum perforatum L. Collected from two sites in North Country
  hadi koohsari hamide khormali ayeshe khormali
  This study was carried out to evaluate the phytochemical, antioxidant and antibacterial activity of Hypericum perforatum L., the aerial parts of plant were collected from two sites in Mazandaran province (Farahabad Sari and Pashakola Amol) during July to October 2015. T More

  This study was carried out to evaluate the phytochemical, antioxidant and antibacterial activity of Hypericum perforatum L., the aerial parts of plant were collected from two sites in Mazandaran province (Farahabad Sari and Pashakola Amol) during July to October 2015. Total phenol and flavonoid contents were determined by colorimetric method using the Folin-Ciocalteu reagent and aluminum chloride respectively. The antioxidant activity was measured by DPPH method and then the antibacterial activity was performed by agar well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extract were determined by broth micro dilution tube method. The results were showed that the total phenolic and flavonoid content and then the antioxidant capacity of plant from Sari region was more than in Amol region. The  MIC values which obtained fromSari  against Staphylococcus aureus, Bacillus cereus, Shigella dysentery and E. coli were 12, 12, 190 and 390 µg/ml respectively, while these values from Amol were 24, 24, 780 and 1560 µg/ml respectively, which results were also confirmed in agar well diffusion method and then in conclusion the plant extract from Sari region had the highest content of secondary metabolites , antioxidant and antibacterial activity against tested bacteria and there is the direct correlation between phyto chemistry and their antioxidant and antibacterial activity of plant.   Manuscript profile
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  5 - Synthesis and evaluation of antibacterial properties of green copper oxide nanoparticles from Hypericum perforatum plant extract and Marrubium Vulgare
  Ashkan Farazin Shirin Kavezadeh
  10.22034/jna.2022.1951530.1291
  In the present study, copper oxide nanoparticles were synthesized using Hypericum perforatum plant extract from the Malpican family and Marrubium Vulgare plant from the mint family. Since it is believed that the antioxidants in the plant reduce the reduction of metal io More

  In the present study, copper oxide nanoparticles were synthesized using Hypericum perforatum plant extract from the Malpican family and Marrubium Vulgare plant from the mint family. Since it is believed that the antioxidants in the plant reduce the reduction of metal ions to nanoparticles as reducing agents, these two plants were tested for their antioxidant properties by the free radical scavenging method, and the IC50 quantity was measured. Hypericum perforatum plant with IC50 equivalent to 0.413 had more antioxidant content than Marrubium Vulgare plant with IC50 equivalent to 1.562, so it was superior in the process of green synthesis. The properties of the synthesized nanoparticles were analyzed using X-ray diffraction (XRD), Scanning electron microscope (SEM), and Ultraviolet-visible (UV-Visible). The presence of a metal-oxygen bond was confirmed by Fourier-transform infrared spectroscopy (FTIR). X-ray energy diffraction spectra showed the purity of the synthesized nanoparticles. The synthesized nanoparticles were observed with spherical morphology and size distribution of 30 to 40 nm and with uniform size distribution. The results of the XRD spectrum showed that pH adjustment did not affect the synthesis of copper oxide nanoparticles. The nanoparticles synthesized against the two bacteria used in this present study did not show significant antibacterial properties compared to industrial antibiotics. Manuscript profile
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  6 - Study the antimicrobial effects of methanolic extract of Hypericum perforatum on foodborne bacteria
  Leila Ghodrati Mehrdad Ataie Kachoie Sadegh Mousavi-Fard Fariborz Moattar
  10.30495/jfm.2021.682561
  Hypericum perforatum is a medicinal plant of the genus Hypercasia that has phenolic and flavonoid compounds with high antimicrobial and antioxidant properties. The present study was performed to evaluate the antimicrobial effects of the methanolic extract of Hypericum p More

  Hypericum perforatum is a medicinal plant of the genus Hypercasia that has phenolic and flavonoid compounds with high antimicrobial and antioxidant properties. The present study was performed to evaluate the antimicrobial effects of the methanolic extract of Hypericum perforatum on food-borne bacteria. Aerial part of Hypericum perforatum was prepared from the research farm, and after approval by experts, it was dried and powdered and used to prepare methanolic extract. The diameter of the growth inhibition zone of Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus was assessed using disk diffusion and compared with antibiotics. Minimum inhibitory concentration (MIC) and Minimum Bacterial Concentration (MBC) of methanolic extract of Hypericum perforatum were evaluated on the target bacteria using ELISA plate. The diameter of the growth inhibition zone of bacteria against the methanolic extract of Hypericum perforatum ranged from 9.33±0.45 to 15.28±0.60 mm. Application of 100 mg/ml concentration of methanolic extract of Hypericum perforatum caused the highest diameter of the growth inhibition zone of Pseudomonas aeruginosa (12.27±0.53 mm), Escherichia coli (13.20±0.59 mm) and Staphylococcus aureus (15.28±0.60 mm). The antimicrobial effects of methanolic extract of fenugreek were dose-dependent (P <0.05). The lowest and highest levels of MIC and MBC of methanolic extract of Hypericum perforatum were obtained for Staphylococcus aureus (0.0010 and 0.0019, respectively) and Escherichia coli (0.50 and 1.00, respectively). The diameter of the growth inhibition zone of bacteria treated with methanolic extract of Hypericum perforatum was higher compared to some antibiotic discs. Manuscript profile
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  7 - اثر گل راعی و پروبیوتیک بر عملکرد، صفات لاشه و مورفولوژی روده در بلدرچین ژاپنی
  مصطفی بنی شریف فرشید خیری سید محمدعلی جلالی
  خلاصه و هدف: گل راعی گیاه بومی ایران است. این گیاه یکی از مهمترین گیاهان با تاثیرات متنوع دارویی می باشد. این آزمایش برای بررسی اثرات تغذیه گل راعی و پروتکسن روی عملکرد، خصوصیات لاشه و مورفولوژی روده بلدرچین ژاپنی انجام شد. روش تحقیق : آزمایشی با240 قطعه جوجه ده روزه ب More

  خلاصه و هدف: گل راعی گیاه بومی ایران است. این گیاه یکی از مهمترین گیاهان با تاثیرات متنوع دارویی می باشد. این آزمایش برای بررسی اثرات تغذیه گل راعی و پروتکسن روی عملکرد، خصوصیات لاشه و مورفولوژی روده بلدرچین ژاپنی انجام شد. روش تحقیق : آزمایشی با240 قطعه جوجه ده روزه بلدرچین ژاپنی باهشت تیمار وسه تکرار در قالب طرح کاملا تصادفی انجام شد. تیمارها به تیمار شاهد (بدون پروتکسین وگل راعی)،2/0درصد( (h14/0درصد(h2)و 6/0 درصد(h3)  با پروتکسین (p1) و بدون  پروتکسین (po) استفاده شد. وزن زنده ،مصرف خوراک  و ضریب تبدیل خوراکی  در هر هفته اندازه گیری شد.  در پایان  آزمایش  دو بلدرچین (نر)  از هر تکرار کشتار و  صفات لاشه  به صورت مجزا محاسبه شد. نتایج: داده ها نشان داد استفاده از گل راعی  و پروتکسین  نسبت به  تیمار شاهد مصرف خوراک اقزایش یافت. همچنین وزن بدن و وزن لاشه در تیماری که گل راعی وپروتکسین  استفاده کرده بودند بیشتر از گروه شاهد  بود. ضریب تبدیل  غذایی  بین تیمارها تفاوت معنی داری(05/0>P) نداشت. توصیه های کاربردی/ صنعتی : بررسی ها نشان داد با افزودن سطوح متفاوت گل راعی به جیره غذایی وزن بدن بادرچین ژاپنی افزایش یافت، که این گیاه دارویی را می توان برای این صنعت توصیه نمود. Manuscript profile
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  8 - اثر عصاره ی گل راعی (Hypericum perforatum L.) بر مورفومتری اندام های گوارشی در موش های صحرایی نژاد ویستار
  مریم مدحج صالح طباطبایی وکیلی محسن ساری محسن ساری اسرافیل منصوری
  مقدمه و هدف: گل راعی یا هوفاریقون (Hypericum perferatum L.) حاوی هایپرسین و هایپرفورین می باشد که با رسپتورهای اوپیوئیدی و گابا باند می شوند. اثرات فارماکولوژیک متعددی مانند ضددردی، آرام بخشی، تسکین دهنده، ضد اسپاسم، ضد تشنج، ضد افسردگی و ضدباکتریایی آن به اثبات رسیده ا More

  مقدمه و هدف: گل راعی یا هوفاریقون (Hypericum perferatum L.) حاوی هایپرسین و هایپرفورین می باشد که با رسپتورهای اوپیوئیدی و گابا باند می شوند. اثرات فارماکولوژیک متعددی مانند ضددردی، آرام بخشی، تسکین دهنده، ضد اسپاسم، ضد تشنج، ضد افسردگی و ضدباکتریایی آن به اثبات رسیده است. در این پژوهش دو سطح چای کوهی  mg/kg.bw)250 و 500) بر مورفومتری اندام­های گوارشی رت­های ویستار بررسی شد. روش تحقیق: از میان 54 سر موش آزمایش با 6 تکرار، اثر عصاره هوفاریقون در سه تیمار ارزیابی شد. 3 سر موش از هر تیمار جهت مورفومتری معده، روده کوچک و بزرگ و نیز طحال به طور تصادفی انتخاب شدند. تیمارها عبارت از گروه کنترل (دریافت کننده آب مقطر)، گروه دو و سه به ترتیب دریافت کننده عصاره­ی هوفاریقون سطح mg/kg.bw)250 و 500) بودند. موش­ها به مدت30 روز و به صورت یک روز در میان، عصاره هوفاریقون را از طریق گاواژ دریافت کردند. در انتهای آزمایش پس از بی هوشی، اندام­های مورد نظر ارزیابی شدند. نتایج و بحث: یافته­ها حاکی از آن بود که سطحmg/kg.bw  250 عصاره در مقایسه با گروه کنترل، باعث افزایش معنی دار وزن روده بزرگ شده است (p < 0.05). هم­چنین طول روده بزرگ در سطحmg/kg.bw  250 و 500 در مقایسه با گروه کنترل افزایش معنی دار داشته است ( p ≤ 0.05).  توصیه کاربردی/ صنعتی:  با توجه به نتایج حاصل از تحقیق حاضر می توان از این گیاه در افزایش وزن استفاده کرد. Manuscript profile
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  9 - Investigation of the Changes in the Amount of the Secondary Essential of Hypericin in Hypericum perforatum L. in Different Highlands of Golestan National Park, Iran
  Parinaz Asghari Seyedeh Khadijeh Mahdavi Seyed Ali Hosseini Masumeh Mazandarani
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  10 - Effect of Salicylic acid on phytochemical charactrization in medicinal plant (Hypericum perforatum L.) under salt stress
  Saber Afzali gorouh Mahmood solouki Masoud Nejati
  به منظور بررسی اثر متقابل شوری و اسیدسالیسیلیک بر خصوصیات فیتوشیمیایی در گیاه دارویی علف‌چای، آزمایشی گلدانی، در گلخانه تحقیقاتی دانشکده کشاورزی دانشگاه زابل واقع در سد‌سیستان در سال زراعی 95-94 به اجرا درآمد. در این آزمایش، به گیاهان با غلظت 50 میلی مولار کلرید سدیم تن More

  به منظور بررسی اثر متقابل شوری و اسیدسالیسیلیک بر خصوصیات فیتوشیمیایی در گیاه دارویی علف‌چای، آزمایشی گلدانی، در گلخانه تحقیقاتی دانشکده کشاورزی دانشگاه زابل واقع در سد‌سیستان در سال زراعی 95-94 به اجرا درآمد. در این آزمایش، به گیاهان با غلظت 50 میلی مولار کلرید سدیم تنش اعمال شد سپس با غلظت 5/0 میلی مولار اسیدسالیسیلیک محلول پاشی شدند (S2SA3). یک گیاه هم به عنوان شاهد (بدون تنش کلریدسدیم و محلول پاشی اسیدسالیسیلیک)، به منظور مقایسه تغییرات ترکیبات شیمیایی و درصد آن‌ها در گیاه انتخاب شد (S1SA1). آنالیز ترکیبات اسانس با GC/MS در تیمار شاهد (S1SA1) نشان داد که 20 نوع ترکیب شیمیایی در سرشاخه‌ها و برگ‌های گیاه وجود دارد. در این تیمار، ترکیبات 1و6- سیکلودکادین، 1-کاریوفیلن بی سیکلو و سزکوئی‌ترپن اسپاتلنول به ترتیب 92/25، 69/16، 38/11 درصد، اجزای غالب اسانس را تشکیل می‌دادند و ترکیبات 3 و4 دی متیل هپتان و آلفاپینین کمترین میزان را داشتند. در تیمار (S2SA3) در اسانس این گیاه، 30 نوع ترکیب شیمیایی شناسایی شد که سزکوئی‌ترپن‌های ژرماکرن و بتاکاریوفیلن به ترتیب 58/15، 79/11 درصد و ترکیب غیرترپنی 2- متیل اکتان 46/10 درصد، اجزای غالب اسانس را تشکیل می‌دادند. ترکیبات نفتالن، تائوکادینول و 1- هگزادکانول نیز کمترین میزان را نسبت به سایر ترکیبات داشتند. تغییرات میزان (درصد) ترکیبات در دو تیمار تفاوت زیادی باهم دارند. میزان این ترکیبات در تیمار شاهد (S1SA1) و تیمار (S2SA3) به ترتیب شامل: کاریوفیلن 93/0 و 74/6، فیتول 03/6 و 38/1، بتاکاریوفیلن 60/2 و 79/11، نفتالن 40/2 و 84/1، آلفاپینن 36/2 و23/1، دلتاکادینن 89/1 و 81/0درصد می‌شوند. تفاوت در میزان ترکیبات به عوامل مختلفی همانند عوامل محیطی وتنش‌ها، نحوه برداشت و خشک کردن گیاه و... بستگی دارد. در این میان محلول‌پاشی اسیدسالیسیلیک با تعدیل کردن اثرات سو تنش شوری موجب بهبود و افزایش درصد و همچنین افزایش ترکیبات شیمیایی موجود در اسانس گیاه علف‌چای می‌شود. Manuscript profile