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      • Open Access Article

        1 - Investigation of different selenium sources on phytochemical characteristics of Echium amoenum fisch. & C.A. Mey. as a medicinal herb of Iran
        Mazaher Hosseinzadeh Rostam Kalaei Vahid Abdossi Elham Danaee
      • Open Access Article

        2 - امکان سنجی غنی سازی دمنوش گل گاو زبان با استفاده از گل ختمی سیاه و پنیرک
        افشین جعفرپور درسا نبی بخش
        مقدمه و هدف: گیاه گل گاوزبان معمول­ترین دم کردنی پس از چای و با ارزش تغذیه­ای فراوان می­باشد. هدف از این تحقیق تولید دمنوش گل گاوزبان غنی سازی شده توسط پنیرک و ختمی سیاه، به منظور ارتقاء خواص حسی و قدرت آنتی اکسیدانی دمنوش می­باشد. روش تحقیق:در این تحقیق More
        مقدمه و هدف: گیاه گل گاوزبان معمول­ترین دم کردنی پس از چای و با ارزش تغذیه­ای فراوان می­باشد. هدف از این تحقیق تولید دمنوش گل گاوزبان غنی سازی شده توسط پنیرک و ختمی سیاه، به منظور ارتقاء خواص حسی و قدرت آنتی اکسیدانی دمنوش می­باشد. روش تحقیق:در این تحقیق بررسی خاصیت آنتی اکسیدانی با استفاده از روش DPPH  با غلظت 5-10×6 مولار در 5/1 میلی­لیتر از عصاره الکلی انجام شد. میزان کدورت دمنوش­­­ها با استفاده از دستگاه اسپکتروفتومتر در طول موج 800 نانومتر تعیین گردید. آزمون خاکستر کل، خاکستر نا­محلول در اسید، میزان رطوبت و ارزیابی حسی دمنوش نیز طبق استاندارد انجام پذیرفت. میزان رطوبت کل نمونه­ها بیشتر از محدوده استاندارد گل گاوزبان بود و این به آن دلیل است که برای آسیاب کردن بهتر گل گاوزبان آن را قبل از فرآیند بسته­بندی مجددا خشک می­نمایند تا رطوبت محیط زدوده شود. نتایجو بحث: نتایج نشان داد که تمام نمونه­ها از قدرت آنتی اکسیدانی بالایی برخوردار بودند، اما نمونه­ای که شامل 0% پنیرک، 30% ختمی و 35% گل گاوزبان بود بیشترین قدرت مهار رادیکالی را داشت، که مقدار آن 04/95% بود(05/0>p)؛ همچنین در ارزیابی حسی، این نمونه بالاترین رتبه را با اختلاف معناداری نسبت به سایر نمونه­ها به خود اختصاص داد، بنابراین می­توان نتیجه گرفت که این نمونه مناسب­ترین ترکیب برای تولید دمنوش مورد نظر از لحاظ خواص حسی و قدرت آنتی اکسیدانی است. توصیه کاربردی صنعتی: نتایج این پژوهش نشان داد که استفاده از گل گاوزبان و ختمی در تهیه دمنوش، محصولی جدید با خاصیت آنتی اکسیدانی بالا، و خواص حسی مطلوب فراهم می‌کند. Manuscript profile
      • Open Access Article

        3 - Evaluation of the effect of different types and concentrations of growth regulators on callus production in in vitro cultivation of Echium Amoenum
        Hamed Arastegi leila Ghaffarzadeh namazi hasan maleki Rasul Asghari-Zakaria shima bourang
        Echium Amoenum is one of the most valuable medicinal plants in traditional and modern medicine and is effective in treating many diseases. The presence of some valuable medicinal compounds in this plant has caused the attention of many scientists in the field of biology More
        Echium Amoenum is one of the most valuable medicinal plants in traditional and modern medicine and is effective in treating many diseases. The presence of some valuable medicinal compounds in this plant has caused the attention of many scientists in the field of biology and medicine. The utilization of plant tissue and cell culture methods, including callus culture and cell suspension, is one of the effective methods in culture and increase the effective compounds of this valuable plant. In this study, we investigated the optimal conditions for the production of callus tissue from leaf and stem explants obtained from seedlings grown from seed culture. According to the results, significant difference was observed between control samples (MS free culture) and other treatments in terms of callus formation percentage and fresh callus weight. The highest percentage of callus formation (100%) and the highest fresh weight of callus (4.23 g) related to stem explants grown on MS medium containing 0.1 mg / l BAP and 1 mg / l NAA Was. Manuscript profile
      • Open Access Article

        4 - Extraction of bioactive compounds and determination of antioxidant activity Echium amoenum
        Simin Arabi Assal Farrokh Eslamlou Zahra Ezzatpour Ghadim sarvenaz Maghsoodi
        Introduction: Plants are potential sources of natural antioxidants and produce various antioxidative compounds to counteract reactive oxygen species. Consumption of plant derived antioxidant contributes to reducing risks of certain chronic and degenerative diseases. Ech More
        Introduction: Plants are potential sources of natural antioxidants and produce various antioxidative compounds to counteract reactive oxygen species. Consumption of plant derived antioxidant contributes to reducing risks of certain chronic and degenerative diseases. Echium amoenum also named as Gol-e-gavzaban in persian, is one of the most important species of Boraginaceae family and commonly found in the North of Iran. Echium amoenum is usually used as herbal tea in Iran for conditions such as common cold, bronchitis, sore throat, cough, stress and fatigue. The dried violet–blue petals of Iranian Borage also possess diaphoretic, diuretic, sedative, mood enhancer, antifebrile, laxative, anti-depressant, cardiotonic and blood rectifying properties. Recently, this plant is known as an effective factor against cancer. The present study was conducted at Islamic Azad University of Tehran Medical Sciences. The aim of the present study was to determine total phenolic and flavonoid compounds and antioxidant activity of various extracts of Echium amoenum from Kelardasht region.Experimental: E. amoenum plants were collected from Kelardasht region in Mazandaran province, Iran (2020, June) at the flowering stage, identified by the herbarium of the Research Institute of Forest and Rangelands. The aerial of E. amoenum dried in shade at room for 6 days and ground to a fine powder. The plant sample (50 g) was extracted using Ethanol and Acetone solvents in different concentrations by maceration method. Afterward, the ethanolic and acetonic extracts of E. amoenum were filtered through Whatman No.1 filter paper. The ethanolic extract was evaporated to dryness under reduce pressure (rotary evaporator Buchi R-300, Switzerland) and acetonic extract was concentrated by vaccum oven (Memmert VO49, Germany) at 35°C. Then the extracts were dried by freeze dryer (UO11, Iran) at -40°C and were pulverized. In this study, extraction of total phenolic compounds from E. amoenum by Acetone and Ethanol solvents in three different concentrations (pure, 50% and 75%) were investigated. Total phenolic contents were determined by Folin- Ciocaltue assay and were expressed as mg of gallic acid per g of extract. Then the antioxidant capacity of extracts were assessed by DPPH radical-scavenging activity and reducing power assay and compared with BHT and ascorbic acid standardsResults and Disscussion: In all extracts, the highest total phenolic contents were observed in 50 percentage concentration so that in these conditions, total phenolic contents of acetonic and ethanolic extracts were obtained as 82.9 and 77.3 mg gallic acid equivalents (GAE)/g extract, respectively, ( p ≤ 0.05). Extracts showed antioxidant activity in a dose-dependent manner. Acetonic extract had more antioxidant activity and DPPH radical-scavenging than ethanolic exrtact. The increase antioxidant activity of the plant can be attributed to the high amounts of phenolic and flavonoid compounds.Extension: Echium amoenum can be used as a potential source of phenolic compounds and natural antioxidant. According to the obtained results based on the high antioxidant properties of this plant, it is suggested that with more researches on extract of E. amoenum can be used more and more in the food industry to prevent the oxidative deteriorative activity of food materials. Manuscript profile
      • Open Access Article

        5 - Eachium amoenum as a functional food ingredient
        Mona Torabi-Toran-Pashtoshti vajihe Fadaei Mohammad reza Eshaghi
        Introduction:  Medicinal plants, either alone or in combination with other drugs, have a growing trend in the treatment of humans. Echium amoenum is one of the annual plants from the Boraginaceae family that in Iran, it is famous as the Gol-e-Gavzaban and is the on More
        Introduction:  Medicinal plants, either alone or in combination with other drugs, have a growing trend in the treatment of humans. Echium amoenum is one of the annual plants from the Boraginaceae family that in Iran, it is famous as the Gol-e-Gavzaban and is the one of the most important medicinal plants in Iranian traditional medicine.  The leaves and flowers organize the useable parts of Gol-e-Gavzaban. In Iranian traditional medicine; the petals of this plant have been used because of strengthening and relaxation effects. The leaves of Gol-e-Gavzaban, with scientific name Borago officianals, have a tongue leaf and full of delicate thorns that gives the rough case to it; and because of this, it is known as Gavzaban. The purpose of this review is the summary description of pharmaceutical effects and functional food and bio-active compounds for introducing of it as a functional food in food industry usage.Results and Disscussion: Echium amoenum like other medicinal plants number the important source of chemical compounds with the potential curative effects. The main bio-active compounds that extracted from Eachium amoenum are poly phenols, Anthocyanidin, Flavonoids, Strols, Saponins, Unsaturated Triphenoids, Unsaturated fatty acids and minor amounts of Pyrrolizidine alkaloid. Rosmarinic acid with Anti_inflammatory effects and Antiviral effects is the main compound of Polyphenols in Gol-e-Gavzaban .This plant contains bioactive compounds that have antioxidant, antibacterial, antiviral, anti-diabetic, anti-inflammatory, analgesic and immune system regulating effects. It was noted that it should be moderated in consumption of this medicinal plant.Extension: Echium amoenum with a wide range of medicinal activities can be considered as a health component. Since the consumption of plant extracts is not pleasant for most people due to their special flavor and odor, so they can be used in combination with food and benefit from their beneficial effects. Also, due to the positive effect of Echium amoenum on the survival of probiotic bacteria, it can be used in the production of synbiotic food. Manuscript profile