Evaluation of the effect of different types and concentrations of growth regulators on callus production in in vitro cultivation of Echium Amoenum

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Hamed Arastegi ¹ (1- MSc. student of Horticultural Engineering-Medicinal Plants, Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili,Iran.)
leila Ghaffarzadeh namazi ² (iran ardabil university of mohaghegh ardabili)
hasan maleki ³ (3- Assistant Professor, Horticulture - Physiology and breeding of flowers and ornamental plants, University of Mohaghegh Ardabili, Ardabil, Iran)
Rasul Asghari-Zakaria ⁴ (Professor, Department of Plant Production Engineering and Genetics Department, University of Mohaghegh Ardabili, Ardabil, Iran.)
shima bourang ⁵ (PhD student of Agricultural Biotechnology, University of Mohaghegh Ardabili, Ardabil, Iran)

Received: 2021-11-22 Accepted : 2022-06-10 Published : 2022-11-22

Keywords: tissue culture, BAP, NAA, Echium Amoenum, callus culture,

Abstract :

Echium Amoenum is one of the most valuable medicinal plants in traditional and modern medicine and is effective in treating many diseases. The presence of some valuable medicinal compounds in this plant has caused the attention of many scientists in the field of biology and medicine. The utilization of plant tissue and cell culture methods, including callus culture and cell suspension, is one of the effective methods in culture and increase the effective compounds of this valuable plant. In this study, we investigated the optimal conditions for the production of callus tissue from leaf and stem explants obtained from seedlings grown from seed culture. According to the results, significant difference was observed between control samples (MS free culture) and other treatments in terms of callus formation percentage and fresh callus weight. The highest percentage of callus formation (100%) and the highest fresh weight of callus (4.23 g) related to stem explants grown on MS medium containing 0.1 mg / l BAP and 1 mg / l NAA Was.