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Open Access Article
1 - Plaque formation by Newcastle virus strain V4 on cell culture and characterization with RT-PCR
Sobhani, S., Mehrabanpour, M.J. .. Cloned vaccines are used in many countries nowadays. One of the ways for cloning a virus is propagation of the virus on cell culture to obtain discrete different plaques in order to study their morphology and genetics. In this study monolayer Madin-Darby Canine Kidne More. Cloned vaccines are used in many countries nowadays. One of the ways for cloning a virus is propagation of the virus on cell culture to obtain discrete different plaques in order to study their morphology and genetics. In this study monolayer Madin-Darby Canine Kidney (MDCK) cell cultures were prepared by standard method. Various dilutions of the viruses were inoculated into monolayer MDCK cell cultures that were supplemented with magnesium sulfate and trypsin, and over laid with agar medium. The viruses could reproduce on these cells and caused cytopathic effect and plaques. At 10-6 virus dilution, 6 various shape and size discrete plaques were obtained and inoculated into allantoic fluid 9-11 days embryonated eggs. After 48 hrs, the allantoic fluids contain plaques were harvested and their RNA extracted. Cleavage site of fusion protein, with RT-PCR test was performed and the PCR products were purified and sequenced. The sequences of nucleotides and amino acids for each plaque were compared with those of the registered strain at gene bank as well as with each other. Molecular studies showed that all plaques are lentogenic strain of Newcastle disease virus and has about 97% to 99% homology with the strain V4 in the gene bank. The aim of this study is produce clear plaque by V4 strain of NDV on MDCK cell line and studies the molecular variations among them. Manuscript profile -
Open Access Article
2 - Study of rosemary extract on BCL2 and BAX gene expression in canine mammary gland carcinoma cell line (CF41.Mg)
Shabani, S., Mortazavi, P. .Apoptosis is named genetically programmed cell death and have special role in physiological and pathological conditions. BAX protein as a key protein in the apoptosis induced by various factors in the apoptosis pathway acts and BCL-2 have anti-apoptotic effect in respon MoreApoptosis is named genetically programmed cell death and have special role in physiological and pathological conditions. BAX protein as a key protein in the apoptosis induced by various factors in the apoptosis pathway acts and BCL-2 have anti-apoptotic effect in response to various stimuli of the mitochondrial apoptosis by preventing the release of cytochrome C applies. In this study, the effect of rosemary extracts on the expression of BCL-2 and BAX genes on breast cancer cells dogs (CF41.Mg). in in vitro examined and the control group (which cell line CF41.Mg without drugs) were compared Cells at doses of 5,10,25,50 and 100 micromole rosemary extract for 24,48 and 72 hours were exposed then by using MTT, the effect of drug on cell survival were analyzed. The tests in which different levels of rosemary extract was added to the cell culture, it was determined that rosemary extract induced cell death after 48 hours’ maximum amount to be allocated. The result suggests that the antitumor activity of the extract after 48 hours at a dose 25 mg/ml is more effective. The results of this study confirm the inhibitory effect of rosemary extract on canine mammary tumor cells (CF41.Mg). Manuscript profile -
Open Access Article
3 - Anticancer Effects of Glucan Polysaccharide of Fusarium spp and Its Chemical Analysis by FT-IR and HPLC
, B. Salehi , M. Bayat , M. Dezfulian , A Sabokbar , B. TabaraieCancer is the second factor of death in world on the basis of WHO reports on 2018. Application of fungal polysaccharides is one of the cheaper, less dangerous, less side effects, and newer in the treatment and prophylaxis of cancer. Object of this research is isolation MoreCancer is the second factor of death in world on the basis of WHO reports on 2018. Application of fungal polysaccharides is one of the cheaper, less dangerous, less side effects, and newer in the treatment and prophylaxis of cancer. Object of this research is isolation and identification of endemic fusarium fungus, its glucan extraction, determination of chemical characteristics by HPLCand FT-IR and anticancer effects on cell lines of LCL, Hela.In this study fusarium fungus was isolated from soil of karaj district and identified on the basis of microscopic and macroscopic and genetical characteristics as a fusarium genus .Then fungus was grown into selective broth medium for obtaining of the most biomass for extraction of glucan .Glucan of fungus was extracted by boiling water method.Different amounts of extracted glucan were treated to the cell cultures of lcl and Hela and its cytotoxicity effects were surveyed.Results showed that glucan polysaccharide had anticancer effects against cell lines of LCL and no anticancer effects against Hela cell lines. Cytotoxicity effects of glucan showed by colorimetry MTT method. Manuscript profile -
Open Access Article
4 - Identification of a new strain of the respiratory virus in the green iguana (Iguana iguana)
پیمان Mohammadzadeh سجاد MohammadiRecently, nidoviruses have been described as a possible cause of severe respiratory diseases in reptiles and especially pythons from different parts of the world. The aim of this study is to isolate the pathogenic agent along with the precise determination of its charac MoreRecently, nidoviruses have been described as a possible cause of severe respiratory diseases in reptiles and especially pythons from different parts of the world. The aim of this study is to isolate the pathogenic agent along with the precise determination of its characteristics and to examine the histopathological findings in a female iguana. During the iguana post-mortem examination, pyogranulomatous and fibronecrotic lesions were observed in various organs other than the respiratory system, and the results of the reverse transcription polymerase chain reaction were also positive. Therefore, the relationship between the observation of these extensive lesions and the Necropsy findings obtained from Previous cases of serpanovirus infection and the amount and type of changes in the genome of the serpentovirus identified with the previous serpentovirus were investigated. Cell culture inoculation and then RT-PCR was used to collect and obtain the virus isolate. Next, immunohistochemistry was performed. Staining for the nucleoprotein of the serpentine virus showed that this virus infects not only a wide range of epithelia (respiratory and gastrointestinal epithelium, hepatocytes, urinary tubules, pancreatic ducts, etc.), but also contaminates the intravascular monocytes, intralesional macrophages, and endothelial cells too. With next-generation sequencing, the complete genome for this new serpentine virus species was obtained.The analysis of the viral genome recovered from this respiratory and systemic disease associated with serpentine virus infection did not show a sequence correlation with the phenotype of other strains. The results showed that this serpentine virus has a wide cell and tissue tropism, and the course of infection by it can be different, and as a result of the systemic spread of the virus in the body, it causes lesions in a wide range of different body systems. Manuscript profile -
Open Access Article
5 - Studying the effect of co-culture system of Mesenchymal Stem Cells on development of follicles in mice ovary in vitro
Ali Mohammadeini Ahmad Ali Mohammadpour Abbas ParhamObjective: Many researchers are trying to clarify necessary hormonal factors for structural distinguishability and functional activities of ovarian tissue by using cell culture and tissue culture techniques. Hence, in this study, we examined ovarian tissue culture on me MoreObjective: Many researchers are trying to clarify necessary hormonal factors for structural distinguishability and functional activities of ovarian tissue by using cell culture and tissue culture techniques. Hence, in this study, we examined ovarian tissue culture on mesenchymal stem cells isolated from cultured adipose tissue.Materials and methods: In this study, mesenchymal stem cells became isolated from adipose tissue of the mouse. After five days and monolayer formation of Mesenchymal stem cells, obtained ovarian was placed on the cultured Mesenchymal stem cells’ monolayer for 7 days.Results: The results of this study show that numbers of mature follicles for treat group compared to the control group were 61/1±2/2 and 38/1±1/9, respectively which had a significant increase compared to control group (P<0/05). This shows the efficiency of tissue culture in co-culture with Mesenchymal stem cells. The real-time PCR studies also confirmed the decrease in the BAX genes in treatment Groups to compare the control group (P<0/05). According to the results yielded regarding the number and quality of obtained follicles, it seems that this method is efficient and of high importance in producing mature follicles, and subsequently high quality oocytes and embryo. Manuscript profile -
Open Access Article
6 - Evaluation Of the Effects Of Different Concentrations Of Aloe veraExtract on the Proliferation Of Uterus Cervical Cancer Cells(Hela)Of Humans
Elahe Ebrahimi Masoud Parsania Hamid Hosseini dostInroduction and Objective: Cervical cancer is the second most common cancer in womenHigh incidence ofcerviccancer and the side effects of the treatments with synthetic chemical compounds demonstrated that hazards are immense.Considering the side effects, treatment with MoreInroduction and Objective: Cervical cancer is the second most common cancer in womenHigh incidence ofcerviccancer and the side effects of the treatments with synthetic chemical compounds demonstrated that hazards are immense.Considering the side effects, treatment with extracts of plants such as Aloe vera have proved to be reducing the levels of side effects and can bemuch cheaper alternative. So, anti cancer properties of the extracts of Aloe vera against the Hela cells of humanwas evaluated in tissue culture, in this study.Material and Methods:After gatheringand confirmthe desiredplant species.Extractionwas performed bymacerationThe toxicity effects of different concentrations 16,17,18,19, 20 and 21 mg/ml of extract of Aloe veraon the Hela cells of human, were evaluated using Trypan blue and MTT methods,on 24, 48 and 72 hours after exposure to a mediumcontaining 1serum and extractResults: 20 mg/ml concentration ofAloe veraextract was toxical effects on Hela cells of human based on the results obtained, it was concluded that extracts of Aloe verainhibit the proliferation of Hela cells of human Manuscript profile -
Open Access Article
7 - Effects of changes in some electrospinning parameters on fibroin nanofibers morphology
Amirasad Pourabadeh Shaghayegh SadrzadehSilk fibroin (SF) is a natural fibrous protein that has been widely studied for application in the biomedical field as a matrix for tissue engineering. Pure fibroin was extracted from silk cocoon by degumming method using aqueous Na2CO3 solution followed by solubilizing MoreSilk fibroin (SF) is a natural fibrous protein that has been widely studied for application in the biomedical field as a matrix for tissue engineering. Pure fibroin was extracted from silk cocoon by degumming method using aqueous Na2CO3 solution followed by solubilizing in CaCl2-C2H5OH-H2O aqueous solution and frozen in liquid nitrogen, then lyophilized in freeze-dryer. SF fibers with diameters down to the nanometer range are formed by subjecting a fluid jet to a high electric field. The electrospinning of the SF sponge was performed with formic acid, as a spinning solvent, at 7% (w/w) fibroin concentration. In this regard, electrospinning parameters including voltage, flow rate, and distance were used as variable parameters, and the effect of changes in these parameters was investigated. The morphology of SF nanofibers was characterized by SEM. As the flow-rate increased, the available polymer volume was high which increased the nanofiber diameter. Also the lower the solution flow-rate, the smaller the diameter of the resultant electrospun nanofibers and bead defects. With an increase in the distance between the capillary and the collector the nanofiber diameter initially decreased to a minimum and then increased. Manuscript profile -
Open Access Article
8 - کاربرد الیسیتورها برای افزایش تولید متابولیت های ثانویه در سوسپانسیونهای کشت سلول و اندام گیاهی
مریم محمدی فارسانی عبدالله قاسمی پیربلوطیدر زیست فناوری همواره به بررسی مسیرهای جایگزین برای تولید ترکیبات طبیعی توجه میشود. سیستمهای کشت سلول و اندام گیاهی برای تولید متابولیت های ثانویه ای که اهمیت تجاری در صنایع غذایی و دارویی دارند قابل جایگزینی میباشند. با این حال، تقریباً تعداد کمی از محیطهای کشت این Moreدر زیست فناوری همواره به بررسی مسیرهای جایگزین برای تولید ترکیبات طبیعی توجه میشود. سیستمهای کشت سلول و اندام گیاهی برای تولید متابولیت های ثانویه ای که اهمیت تجاری در صنایع غذایی و دارویی دارند قابل جایگزینی میباشند. با این حال، تقریباً تعداد کمی از محیطهای کشت این ترکیبات را سنتز میکنند و میزان این سنتز و مدت زمانی که صرف میکند قابل مقایسه با استفاده از یک گیاه کامل میباشد. روشهایی شامل تغییر محیط کشت (افزودن مواد مغذی و هورمونی) و شرایط محیطی (تغییرات دما، pH و اسمزی) و همین طور از مواد استخراجی و ترکیبی نیز در این راهبردها استفاده می شده است. امروزه از دستکاری ژنتیکی مسیرهای بیوسنتتیک با استفاده از مهندسی متابولیکی که امروزه برای بهبود تولیدات متابولیت های مورد نیاز تکنیکی قدرتمند شده است استفاده میشود. نتایج مطالعات انجام شده نشان میدهد که هم الیسیتورهای زیستی و هم غیر زیستی سنتز متابولیتهای ثانویه را در محیط کشت سلولهای گیاهی افزایش میدهند. Manuscript profile -
Open Access Article
9 - The effect of glucose, sucrose, lactose carbon sources on the growth of Alcaligenes eutrophus for the production of polyhydroxybutyrate
atefeh farjadmanesh seyed ahmad ataeiBackground & Objectives: Polyhydroxyalkanates are a group of polymers that are produced by many bacteria when they enter the growing phase in the presence of mineral sources. The Objective of this study is investigattion of the different culture containing carbon so MoreBackground & Objectives: Polyhydroxyalkanates are a group of polymers that are produced by many bacteria when they enter the growing phase in the presence of mineral sources. The Objective of this study is investigattion of the different culture containing carbon sources of glucose, sucrose, lactose, combination of these carbon sources, on the growth of Alcaligenes eutrophus to produce biodegradable polymers of hydroxy butyrate-valerate. Materials & Methods: In this study Alcaligenes eutrophus was identified In order to producing of hydroxybutyrate-valerate and investigation of the effect of the different carbon sources on the production. To perform experiments, in fed-batch mode, acetic acid and propionic acid combination with acetic acid as a source of volatile fatty acid was added to the culture in a stepwise manner. The culture with inoculated bacteri were transferred to incubator at 32°C, 120 rpm and retention time of 72 hours, for to incubation and polymer production. Results: The results of this study showed that the source of glucose as carbon with acetic acid produced the highest amount of hydroxybutyrate-valerate (HB= 3.4860 g/l and HV=0.7940 g/l). Also, the lowest amount of hydroxybutyrate production (HB= 2.3124 g/l) is Because of using sucrose as the carbon source and the combination of acetic acid and propionic acid. Conclusion: The results showed that Alcaligenes eutrophus used carbon source of glucose and fatty acid source of acetic acid more than other sources of carbon Manuscript profile -
Open Access Article
10 - Fabrication of electrospun silk fibroin scaffold and the effect of its pre-incubation in culture medium on survival and adhesion of rat bone marrow mesenchymal cells
Maryam Janitermi esmail fattahi Seyed Gholam Ali JorsaraiObjective: This study was performed to synthesize electrospun silk fibroin scaffold and to investigate the time-dependent effect of its pre-incubation in culture medium on the adhesion and proliferation of cells seeded on the scaffold. Methods: sericin was removed from MoreObjective: This study was performed to synthesize electrospun silk fibroin scaffold and to investigate the time-dependent effect of its pre-incubation in culture medium on the adhesion and proliferation of cells seeded on the scaffold. Methods: sericin was removed from silk cocoon and fibrin solution (3% w/v) was prepared using formic acid. Then, electrospun silk fibroin scaffold was made using lab-scale electrospinning machine and evaluated by scanning electron microscopy. Pre-incubation of scaffolds in culture medium was performed for 0, 1, 6, and 10 days and the hydrophilicity of scaffolds was evaluated by measuring the water contact angle. Rat bone marrow mesenchymal cells were then isolated, cultured, and seeded on scaffolds. After 21 days of cell seeding, cell viability (MTT method) and genomic DNA concentration of cells attached to the scaffold were evaluated. Results: The results showed that increasing the pre-incubation time in the culture medium decreased the water contact angle and increased the survival and proliferation of cells. In general, the present study showed that pre-incubation of electrospun fibroin scaffold with constant elasticity in culture medium leads to increased scaffold hydrophilicity and consequently increases the proliferation and survival of mesenchymal cells seeded on it. Conclusion: these findings can be used as an effective factor in seeding cartilage cell on scaffolds in tissue engineering. Manuscript profile -
Open Access Article
11 - شبیه سازی کشت سلول توسط Bioreactor بستر بسته بندی شده
مهیار خرم -
Open Access Article
12 - Characterization of functionally graded hydroxyapatite/TiO2 multilayer coating on Ti-6Al-4V titanium alloy by electrophoretic deposition
محمد جعفر هادیان علیرضا عراقی طاهره طلایی مهسا ثانیSingle layer and functionally graded coatings of hydroxyapatite and TiO2 particles were deposited on Ti-6Al-4V titanium alloy substrate by electrophoretic deposition technique (EPD). The coatings chemical composition and morphology were investigated using energy dispers MoreSingle layer and functionally graded coatings of hydroxyapatite and TiO2 particles were deposited on Ti-6Al-4V titanium alloy substrate by electrophoretic deposition technique (EPD). The coatings chemical composition and morphology were investigated using energy dispersive X-ray spectroscopy (EDX), scanning electron microscope (SEM) and X-ray diffraction (XRD) methods. The results showed in HA/TiO2 functionally graded coating, the composition varied from inner layer to top layer as 100 %wt TiO2 to 100 %wt HA. To investigation biocompatibility of the coatings, the culture Wharton’s Mesenchymal Stem Cells was used. The results showed the HA/TiO2 functionally graded coating and HA single layer coating were more biocompatible in comparison to TiO2 single layer coating. The adhesion strengths of the coatings were measured by shear testing and the results showed that the HA/TiO2 functionally graded coating has more adhesion strength (~31 MPa) compare to HA single layer. Manuscript profile -
Open Access Article
13 - Culture and proliferation of dendritic cells from mouse bone marrow progenitors
سمانه عرب معصومه معتمدی جمشید حاجتیDendritic cells (DCs) are the most potent antigen-presenting cells that induce and control the immune responses. These cells have the capacity of stimulation different types immune response (type 1 or 2) in exposure to peripheral signals. The aim of this study was DCs c MoreDendritic cells (DCs) are the most potent antigen-presenting cells that induce and control the immune responses. These cells have the capacity of stimulation different types immune response (type 1 or 2) in exposure to peripheral signals. The aim of this study was DCs culture, proliferation and maturation from mouse bone marrow progenitors. Bone-marrow derived DCs (BMDCs) were cultured in the presence of GM-CSF and IL-4. After 5 days, LPS and cholera toxin were added to the culture for another 2 days. Expression of surface molecules and cytokine production in BMDC culture evaluated. The result shown that LPS stimulated DCs were matured. Exposure with CT induce IL-10 and with LPS induce IL-12 production by DCs. These fundings indicate that 7 days culture of mouce bone marrow progenitors can produce professional dendritic cells and with added different components in DCs culture induce appropriate type 1 or 2 of immune responses. Manuscript profile
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