Anticancer Effects of Glucan Polysaccharide of Fusarium spp and Its Chemical Analysis by FT-IR and HPLC

Subject Areas : Journal of Comparative Pathobiology

, B. Salehi ¹ , , M. Bayat ² , , M. Dezfulian ³ , , A Sabokbar ⁴ , , B. Tabaraie ⁵

1 - Department of microbiology, Faculty of specialized veterinary science, science and researches branch, Islamic Azad University, Tehran, Iran
2 - Department of microbiology, Faculty of specialized veterinary science, science and researches branch, Islamic Azad University, Tehran, Iran.
3 - Department of microbiology, Faculty of Basic Science, Karaj Branch, Islamic Azad University, Karaj, Iran.
4 - Department of microbiology, Faculty of Basic Science, Karaj Branch, Islamic Azad University, Karaj, Iran.
5 - Pasteur Institute of Iran, Tehran, Iran

Received: 2021-05-02 Accepted : 2021-05-02 Published : 2020-06-21

Keywords: Fusarium, Glucan extraction, Cell culture, HPLC, FT-IR,

Abstract :

Cancer is the second factor of death in world on the basis of WHO reports on 2018. Application of fungal polysaccharides is one of the cheaper, less dangerous, less side effects, and newer in the treatment and prophylaxis of cancer. Object of this research is isolation and identification of endemic fusarium fungus, its glucan extraction, determination of chemical characteristics by HPLCand FT-IR and anticancer effects on cell lines of LCL, Hela.In this study fusarium fungus was isolated from soil of karaj district and identified on the basis of microscopic and macroscopic and genetical characteristics as a fusarium genus .Then fungus was grown into selective broth medium for obtaining of the most biomass for extraction of glucan .Glucan of fungus was extracted by boiling water method.Different amounts of extracted glucan were treated to the cell cultures of lcl and Hela and its cytotoxicity effects were surveyed.Results showed that glucan polysaccharide had anticancer effects against cell lines of LCL and no anticancer effects against Hela cell lines. Cytotoxicity effects of glucan showed by colorimetry MTT method.

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