Pain is defense mechanism and occurs when tissue is damaged. Pain receptors in the skin and tissues are free nerve endings. The pain in the tissues is due to the production of a substance called proaglandin, which is reduced by anti-inflammatory drugs. Eliphara plant is More
Pain is defense mechanism and occurs when tissue is damaged. Pain receptors in the skin and tissues are free nerve endings. The pain in the tissues is due to the production of a substance called proaglandin, which is reduced by anti-inflammatory drugs. Eliphara plant is valuable in the food and herbal medicine industry and has a rare medicinal compound called aurantamide. Due to its anti-rheumatic anti-inflammatory effects in the treatment of diseases like arthritis, rheumatism and psoriasis, and by preventing the production of prostaglandins and increasing the production of glutathione, reducing the activity of aspartate aminotransferase, alanine aminotransferase enzyme and reducing the production of TNF-a factor, it reduces side effects. The aim of this study is to investigate the effect of Orantamide drug combination on prostaglandins with molecular docking. PubChem and PDB databases were used to investigate how the drug binds to the active site of the molecule, draw the chemical structure of the drug, energy optimization, binding studies and final analysis. The drug interaction with the receptor was done through different docks using Pyrex, Chimera and Viewer Lite software. The results showed that the desired compound was able to bind the active site of the enzyme and among the software, the best result was obtained from Pirex software. In fact, this compound had the highest level of negative binding energy (-7.5). Therefore, it was shown that the highest affinity for key amino acids is the active site of the enzyme and the site of interaction with the crystal molecule
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In this study the effect of prostaglandin F2 alpha analogue, dinoprost, on corpus luteal tissue in pseudopregnant rabbit was investigated. For this purpose, 42 mature female rabbits were randomly divided into 6 groups of 7 animals each. In 3 groups, o More
In this study the effect of prostaglandin F2 alpha analogue, dinoprost, on corpus luteal tissue in pseudopregnant rabbit was investigated. For this purpose, 42 mature female rabbits were randomly divided into 6 groups of 7 animals each. In 3 groups, on day 10 of pseudopregnancy, dinoprost (1mg/kg) was injected intramuscularly and 24, 48 and 72 hours following injection respectively, the animals were necropsied. In the other three groups, normal saline solution was injected as control groups. In all groups, after blood sampling the animals were necropsied and their ovaries removed and weighed. Following sampling and fixation in 10% formalin solution, the tissue samples were stained by Hematoxylene & Eosine method and the corpora lutea were studied morphologically and morphometrically. Histomorphometrical study indicated that the diameter of corpus luteum decreased significantly (p<0.05) in all treatment groups compared with the control groups. Also progression of connective tissue, pyknotic nuclei, intercellular space and large vacuole formation increased significantly (p<0.05) in treatment groups in comparison with the control groups. Peripheral and central fatty droplets increased significantly (p<0.05) 24 and 48 hours post PGF2α injection respectively. Serum progesterone concentration decreased significantly (p<0.05) in all treatment groups as compared with the control groups. The results indicated that structural and functional luteolysis progressively increased in PGF2α treated groups compared with the control groups.
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This study was performed to compare the effect of using 3 different estrus synchronization methods (Ovsynch, Co-Synch and Prostaglandin) on the conception rate of subfertile dairy cows. Fifty one subfertile Holstein dairy cows with parity ranging from 1 to 7 were enroll More
This study was performed to compare the effect of using 3 different estrus synchronization methods (Ovsynch, Co-Synch and Prostaglandin) on the conception rate of subfertile dairy cows. Fifty one subfertile Holstein dairy cows with parity ranging from 1 to 7 were enrolled in this study. Cows were allocated to three groups Ovsynch (n=10), Co-Synch (n=27) and Prostaglandin (n=14). Cows in the Ovsynch group underwent a standard Ovsynch protocol (GnRH analogue on Day 0, PGF2α analogue on Day 7 and GnRH analogue on Day 9), then they were artificially inseminated approximately 16 hours after the second GnRH treatment. The same procedure was done for cows in Co-Synch group, but artificial insemination took place immediately after the last injection. Cows in prostaglandin group underwent a PG protocol (PGF2α, on Day 0 and 12), and artificial insemination was done about 72 hours after the second PGF2α injection. Pregnancy diagnosis was performed 60 days after insemination via rectal examination. By using logistic procedure of SAS 9.1 software, the effects of treatment, season, parity, animal, age and open days were analyzed. The analysis of variables related to animal and the measured effects on pregnancy proved that none of the evaluated parameters were significant. Hence, we cannot declare which of the estrus synchronization methods is suitable to increase pregnancy rate. Further studies with more replicates are required to choose the best method.
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The objective of this study was to examine the effects of administration of Flunixin Meglumine on conception rates and plasma progesterone(p4) changes in Holestein heifers and inhibition of PGF2a synthesis just before luteo lysis.32 Holstein heifers(15-month-old,B More
The objective of this study was to examine the effects of administration of Flunixin Meglumine on conception rates and plasma progesterone(p4) changes in Holestein heifers and inhibition of PGF2a synthesis just before luteo lysis.32 Holstein heifers(15-month-old,BW:357+_5kg)were synchronized utilizing Ovsynch protocol and artificially inseminated.the heifers were assigned randomly to a treatment group(n=16) and a control group (n=16).the treatment group were injected intramuscularly three times on days 15.5,16 and 16.5 post insemination with 1.1 mg/kg bodyweight Flunixin Meglumine whereas control group received placebo.blood samples were collected on days 15.5,16 and 16.5 post insemination for analysis of plasma P4 concertrations.pregnancy was diagnosed on days 30 and 60 by transrectal ultrasonography.on day 30,10 of the treated heifers were pregnant compared with 4 of the control heifers(p<0.05):on day 60,9 of the treated heifers were still pregnant compared with 4 of the control heifers (p>0.05).maen plasma progesterone concentrations on day 16.5 in treated heifers (2.62+_0.66ng/ml-1) was higher than control group (1.87+_1ng/ml-1) but this improvement was not statistically significant(p>0.1).the results of this study support the hypothesis that Flunixin Meglumine is able to inhibit the uterine synthesis of PGF2a and increase the survival of early embryos.
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