لاکتات

Open Access Article

1 - ارزیابی فعالیت آنزیم‌های شاخص آسیب قلبی پس از حذف پریکارد در خرگوش
O. Samiee Amlashi, , Gh. Abedi محمد Nasrollahzadeh Masouleh, , S. Hesaraki

پریکاردیکتومی یک شیوه درمانی موثر برای برخی از بیماریهای قلبی است و استفاده از بیومارکرهای قلبی شیوه مهمی برای ارزیابی اثرات جراحی بر سلامت قلب هستند. در این راستا، ﻛـﺮاﺗﻴﻦﻛﻴﻨﺎز، آﺳﭙﺎرﺗﺎتآﻣﻴﻨﻮﺗﺮاﻧﺴﻔﺮاز و ﻻﻛﺘـﺎتدﻫﻴﺪروژﻧﺎز ﺑﻪﻋﻨﻮان آﻧﺰﻳﻢﻫﺎی وﻳﮋه آﺳ More

پریکاردیکتومی یک شیوه درمانی موثر برای برخی از بیماریهای قلبی است و استفاده از بیومارکرهای قلبی شیوه مهمی برای ارزیابی اثرات جراحی بر سلامت قلب هستند. در این راستا، ﻛـﺮاﺗﻴﻦﻛﻴﻨﺎز، آﺳﭙﺎرﺗﺎتآﻣﻴﻨﻮﺗﺮاﻧﺴﻔﺮاز و ﻻﻛﺘـﺎتدﻫﻴﺪروژﻧﺎز ﺑﻪﻋﻨﻮان آﻧﺰﻳﻢﻫﺎی وﻳﮋه آﺳﻴﺐ قلبی از اﻫﻤﻴﺖ ﺧﺎﺻﻲ ﺑﺮﺧﻮردارند. هدف از این تحقیق بررسی فعالیت این آنزیمها بعد از پریکاردیکتومی در خرگوشهای سالم بود. تعداد 12 سر خرگوش سفید نیوزلندی نر همسن با وزن تقریبی 3/0±5/1 کیلوگرم به طور تصادفی به دو گروه شاهد و مورد تقسیم شدند. خرگوشهای گروه مورد، عمل پریکاردیکتومی و خرگوشهای گروه شاهد فقط تحت عمل توراکتومی قرار گرفتند. به منظور بررسی تغییرات آنزیمهای کراتینکیناز، لاکتات دهیدروژناز، آسپارتات آمینوترانسفراز، نمونه گیری خون، از تمامی خرگوشهای جراحی شده در هفته های اول، دوم، سوم و چهارم بعد از عمل جراحی، انجام شد. براساس نتایج بهدست آماده، مقادیر کراتین کیناز در هفته ی اول اختلاف معنی داری نسبت به گروه شاهد داشت (05/0 > p). مقادیر کراتین کیناز گروه مورد در هفته اول در مقایسه با هفته های دوم، سوم و چهارم از نظر آماری افزایش معنی داری را نشان داد (05/0 > p). میزان لاکتات دهیدروژناز و آسپارتات آمینوترانسفراز گروه مورد در هفته دوم افزایش معنی داری داشتند (05/0 > p). مقادیر لاکتات دهیدروژناز و آسپارتات آمینوترانسفراز گروه مورد در هفته دوم در مقایسه با هفته های اول، سوم و چهارم اختلاف معنیداری را نشان دادند (05/0 > p). بهطورکلی میتوان نتیجه گرفت که پریکاردیکتومی باعث افزایش موقت فعالیت آنزیمهای شاخص آسیب قلبی در هفته اول و دوم میشود و بعد از پایان هفته دوم سطوح آنزیمهای کراتین کیناز، آسپارتاتآمینوترانسفراز و لاکتات دهیدروژناز به حد طبیعی خود برگشته و این افزایش مقادیر دائمی نیستند. Manuscript profile

Open Access Article

2 - Diagnostic Value of Milk Enzymatic Markers Lactate Dehydrogenase and Alkaline Phosphatase for Subclinical Mastitis in Dairy Cattle
سعید Akbari Aliabad S. O. Ghasemian S. Z. Peighambarzadeh

Mastitis could be diagnosed by diverse methods, including physical, clinical, and laboratory tests. The present study aimed to evaluate the accuracy of subclinical mastitis diagnosis using enzymatic diagnostic tests for milk lactate dehydrogenase (LDH) and alkaline phos More

Mastitis could be diagnosed by diverse methods, including physical, clinical, and laboratory tests. The present study aimed to evaluate the accuracy of subclinical mastitis diagnosis using enzymatic diagnostic tests for milk lactate dehydrogenase (LDH) and alkaline phosphatase (ALP).  This study was performed on 90 Holstein cows selected randomly out of high- and low-yielding dairy cattle. According to the result of the California Mastitis Test, two milk samples were obtained from the intended udder quarters based on the principles of the National Mastitis Council. One specimen was used for microbial culture and the other one was utilized for somatic cell count (SCC), as well as milk LDH and ALP tests by diagnostic kits. Considering microbial culture as the gold standard for subclinical mastitis diagnosis, the sensitivity of SCC, ALP, and LDH tests was 87%, 59.3%, and 70.4%, respectively. Moreover, the specificity of these tests was 77.8%, 80.6%, and 77.8%, respectively. Our findings revealed a strong correlation between SCC, LDH, and ALP. It was observed that an increase in SCC was accompanied by augmented LDH response. According to the results of the present study, SCC was the most efficient test for diagnosing subclinical mastitis in dairy cows, followed by LDH and ALP. Manuscript profile

Open Access Article

3 - Effects of Two Different Amounts of Colostrum Powder Supplementation on Lactate, Blood Glucose and Anaerobic Power in Wrestlers Male
R. Amirsasan T. Bagherpour Goll A. Mootab

Introduction: The aim of this study was to determine the effect of short-term supplementation of two different amounts of colostrum powder on the lactate, blood glucose- and anaerobic power in male wrestler. Materials and Methods: In this applied and quasi experimental More

Introduction: The aim of this study was to determine the effect of short-term supplementation of two different amounts of colostrum powder on the lactate, blood glucose- and anaerobic power in male wrestler. Materials and Methods: In this applied and quasi experimental study, 26 wrestlers (age 21.48± 1.93 years, height 174.64± 6.05 cm, weight 71.96± 10.23 kg, body fat percent 9.78± 3.66 %)   in a randomly and double-blind design were allocated in three equal groups: control (placebo: Dextrose), experimental group 1 (colostrum: 30 g/day) and the experimental group 2 (colostrum: 50 g/day). The training protocol of the present study was to conduct two Wingate tests (30 second) and simulated wrestling with 30 minutes of rest between the two tests. After two weeks of supplementation, the test was repeated under the first phase. Four blood samples before and after two weeks of supplementation (5 ml) were analyzed by ELISA. The result and the parameters (mean and standard deviation) of the four stages were repeated using ANOVA and post hoc tests Bonferuni analysis. All statistical analyses were performed using the SPSS-22 software. The significance level was set at p<0.05. Results: Blood glucose and lactate levels in two experimental groups 1 and 2 and stage one and two were similar to the control group. However, Investigation showed that the mean difference of phase two and four of blood lactate in experimental group 1 and 2 significantly decreased as compared to the control group (P<0/ 05). There was also, a significant difference in the level of anaerobic power in the experimental group 1 and 2 in the post-test (P<0/ 05). Conclusion: Two weeks of supplementation of colostrum powder can increase anaerobic power and decrease the blood lactate in wrestlers. Manuscript profile

Open Access Article

4 - The Effect of Beta-Alanine Supplementation on Performance, Tmax and Blood Lactate of Elite Male Rowers
N. Heydari M. Kashef

Introduction: Rowing is one of the oldest sports and its competition has been held every four years at the Olympic games and is considered as one of the greatest world championship. The aim of this study is to investigate the effect of acute beta-alanine supplementation More

Introduction: Rowing is one of the oldest sports and its competition has been held every four years at the Olympic games and is considered as one of the greatest world championship. The aim of this study is to investigate the effect of acute beta-alanine supplementation on the Tmax, record of 6 minutes on rowing ergo-meter and Vo2max and blood lactate responses in the Iranian national team members of rowing. Materials and Methods: 15 elite male rowers (age: 22.5 ± 4.9, Height: 186.8 ±8.3cm, weight: 80.3 ± 4.9 kg body fat: 8.8± 2.7 percent) voluntarily participated in this study. Rowers were in training camp and preparing for competetion. The subjects were randomly divided into two groups: placebo (control) and supplement groups (experimental). Subjects were supplemented every 4 hours, 3 hours and 2 hours with10 mg per kg of body weight with betaalanine or placebo (glucose) in a cross-over design before the 6-minute and Tmax test on rowing ergo-meter with free rhythm. Tests concerned with rowing ergometer and Tmax and blood lactate before and after 6 minutes test were carried out. Independent t-test and SPANOVA were used for statistical analyses. Results: The results did not show differences among the groups for all the variables (p>0.05), except, Tmax that increased significantly in beta-alanine group. This study showed that acute beta-alanine administration could increase Tmax significantly. Conclusion: According to the results, beta-alanine supplementation could improve rower’s record and postpone their fatigue and improve rower’s stamina to increase the time for exhaustion. Manuscript profile

Open Access Article

5 - The Effect of Selenium Supplementation on Serum Levels of Creatine Kinase, Lactate Dehydrogenase and Muscle Strength at Resting and after a Soccer Match in Young Soccer Players
M. Azimi F. Moradi

Introduction: Few researches have been carried out on the effect of selenium supplementation on muscle damage in athletes. The aim of this study is to investigate the effect of selenium supplementation on serum levels of creatine kinase (CK), lactate dehydrogenase (LDH) More

Introduction: Few researches have been carried out on the effect of selenium supplementation on muscle damage in athletes. The aim of this study is to investigate the effect of selenium supplementation on serum levels of creatine kinase (CK), lactate dehydrogenase (LDH) and muscle strength at resting and after a soccer match in young soccer players. Materials and Methods: The research method was quasi-experimental and 19 young soccer players were selected among soccer players in Saghez city and were randomly divided into two experimental (supplementation, n=10) and control (placebo, n=9) groups. The assessments were carried out in three stages (pre-supplementation, post-supplementation, and immediately after the match). General characteristics of subjects, muscle strength, and circulating levels of LDH and CK were measured. The supplementation period was one month (one 200 µg/ml selenium capsule daily). The placebo group also consumed a starch capsule each day. In order to analyze the data, two factor analysis of variance with repeated measures were used at the significant level of P<0.05. Results: Selenium supplementation has no effect on muscle strength (bench-press p=0.790, leg-press p=0.912) and levels of CK (p=0.051) and LDH (p=0.244) at rest and after the soccer match. Conclusion: Selenium supplementation for one month and a daily intake of 200 μg does not appear to have a beneficial or harmful effect on muscle strength and circulating levels of enzymes indicating muscle damage at rest and following a soccer match in young soccer players. More research is needed to reveal various aspects of the issue. Manuscript profile

Open Access Article

6 - The Response of Oxidative Stress Indicators to Grape Seed Extract Supplementation and Resistance Training in Bodybuilders
Fariba Joshaghani Hamzeh Rahmani Amin Yosefvand

Black grape seed extract (GSE) is a supplement known for its potent antioxidant effects and flavonoid properties that can help prevent or reduce cell damage. This study aimed to examine the impact of grape seed extract supplementation and resistance training on oxidativ More

Black grape seed extract (GSE) is a supplement known for its potent antioxidant effects and flavonoid properties that can help prevent or reduce cell damage. This study aimed to examine the impact of grape seed extract supplementation and resistance training on oxidative stress indicators in bodybuilders. The research followed a semi-experimental design, with participants divided into supplement and control groups for blood sampling before and after supplement intake in a double-blind fashion. The study included 24 male bodybuilders aged 15-19 years. Blood samples were collected during fasting sessions to measure antioxidant capacity (TAC), malondialdehyde (MDA), creatine kinase (CK), and lactate dehydrogenase (LDH) levels. Data distribution normality was assessed using Shapiro-Wilk tests. The results revealed that resistance training in bodybuilders led to a significant increase in cell damage markers (CK and LDH) and MDA levels, along with a notable decrease in TAC (P<0.05). However, grape seed extract consumption was found to boost TAC levels. The study suggests that bodybuilding activities can elevate cell damage and oxidative stress indicators, while daily intake of grape seed extract (100 mg) can enhance total antioxidant capacity in male bodybuilders. Manuscript profile

Open Access Article

7 - The effect of intraperitoneal injection of hydroalcoholic extract of flaxseed on biochemical, hematological and liver enzymes in rats
Atefeh Payez

Background: Medicinal plants have a wide range of applications in various fields, especially in the control of microbial infections, but the discussion of their possible toxicity has caused great concern in the use of these substances in various fields of industry and m More

Background: Medicinal plants have a wide range of applications in various fields, especially in the control of microbial infections, but the discussion of their possible toxicity has caused great concern in the use of these substances in various fields of industry and medicine. This study was performed to clarify some of these ambiguities and to investigate the effect of intraperitoneal injection of flaxseed extract on biochemical, hematological, and hepatic factors in rats.Materials and Methods: In this experimental study, 15 female spiral rats were randomly divided into 5 groups. The first group was the sham group, the second group was the control group, and the third to fifth groups as experimental groups with concentrations of 200, 400, and 600 mg/ml of flaxseed extract were treated, respectively. The mice were treated for 10 days, then on the eleventh day the mice were anesthetized with ether and blood samples were taken from their hearts. Then biochemical parameters, blood, and lactate dehydrogenase, and plasma levels of liver enzymes were evaluated.Results: The results of this study did not show much change in biochemical factors except cholesterol and triglycerides which showed a significant decrease in the extract-treated groups. Blood factors did not show significant changes but increased in high concentrations of liver enzymes.Conclusion: Intraperitoneal injection of flaxseed extract has no effect on blood factors and many biochemical factors in mice, but high concentrations of the extract can affect liver enzymes. Manuscript profile

Open Access Article

8 - Investigating Resistance of Wild Mustard (Sinapis arvensis L.) ‎Populations to Tribenuron-Methyl Herbicide
&lrm; Mehdi Afshari Ali Ghanbari Mehdi Rastgoo Javid Gherekhloo Goodarz Ahmadvand

Tribenuron-methyl is commonly used for post emergence control of broad leaf weeds in wheat fields. In order to survey suspicious resistant weeds in wheat fields to this herbicide thirty-eight fields of Kermanshah province were investigated during 2012- 2013. Seeds of su More

Tribenuron-methyl is commonly used for post emergence control of broad leaf weeds in wheat fields. In order to survey suspicious resistant weeds in wheat fields to this herbicide thirty-eight fields of Kermanshah province were investigated during 2012- 2013. Seeds of suspected resistance of wild mustard were gathered and tested in a randomized complete blocks design experiment with three replications. First, for early detection of herbicide resistance, the suspected population was screened using discriminating dose of tribenuron-methyl. Determining of the resistance degree was conducted by whole plant bioassay tests using dose-response curves. The resistance mechanisms were assayed by molecular methods, especially using the ALS gene cloning by PJET1.2/blunt Vector. For susceptible populations, the concentration required for complete control was 10.4 g ai ha-1 tribenuron-methyl. Also, in screening tests 50% of populations as resistant populations were identified. According to the Beckie and Tardif, it was found that 57.8% of these population did have a very high degree of resistance, 31.5% with high resistance and 10/5% with low resistance degree. GR50 of the resistant weeds was also increased as compared to sensitive weed, which indicates resistance in this province, Thus to control the resistant populations Z15, this amount increased to 1309 g ai ha-1.The results of DNA sequencing showed that mutation by replacing proline amino acid at position Ala122 causes resistance based on target-site mutation. Manuscript profile

Open Access Article

9 - Effect of sodium bicarbonate and beta-alanine supplementation on anaerobic capacity and blood lactate level of female futsal players
Mandana Gholami Marzieh Hafezi eirdmousa sahar ghasem shoar Hossein abednatanzi

10.30495/varzesh.2022.1965942.1041

The main aim of this study was to determine the effect of sodium bicarbonate and beta-alanine supplementation on anaerobic capacity and blood lactate levels of female futsal players. Method: 40 volunteer female futsal players of the Tehran League were selected based on More

The main aim of this study was to determine the effect of sodium bicarbonate and beta-alanine supplementation on anaerobic capacity and blood lactate levels of female futsal players. Method: 40 volunteer female futsal players of the Tehran League were selected based on the variables of entering the research and sampling and randomly selected four of the 40 volunteers and randomly divided into four control groups (n=10), sodium bicarbonate (n=10), beta-alanine (n=10) and sodium bicarbonate and beta-alanine (n=10) were divided. The anthropometric characteristics of their height, weight and body mass index were measured before the test. Then the Wingate test was performed to measure power and their blood lactate level was also measured using a lactometer. After each test session, the bicarbonate group received 0.3 grams of sodium bicarbonate supplement per kilogram of body weight, the beta-alanine group received 0.3 grams of beta-alanine supplement per kilogram of body weight, and the combined group also received per kilogram The combination of beta-alanine and sodium bicarbonate was used for 0.3 grams of body weight, and the control group used 0.3 grams of placebo (starch) per kilogram of body weight, and after 90 minutes, the relevant tests were performed again. Also, the subjects' diet was controlled during the test. Finally, descriptive statistics, Shapiro-Wilk test, one-way analysis of variance test and Tukey's post hoc test were used for statistical analysis of the data using spss/21 software at a significance level of p≤0.05. The results showed that the supplement of sodium bicarbonate and beta-alanine has a significant effect on the average power and blood lactate level of female futsal players. According to the results of the research, it is suggested that coaches and players use sodium bicarbonate and beta-alanine supplements to increase strength and reduce the negative effects of lactic acid. Manuscript profile

Open Access Article

10 - Evaluation of Physicochemical and Microbial Properties of Fresh Tukey Meat Containing Sodium Lactate Stored in Different Types of Multilayer Pouches and Modified Atmosphere
Nazanin Zand Orang Eyvaz Zadeh Farnaz Kalantari

10.30495/jfst.2022.1947794.1769

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11 - Antioxidant enzyme activities and fermentation metabolism in the root of three wheat promising lines under waterlogging stress
Freshteh alizade vaskasi hemmatollah Pirdashti Ali Cherati_Araei Sara Saadatmand

Waterlogging stress has negative effects on the growth and yield of wheat plants, which recognizes the defense mechanism of the plant against waterlogging, can be valuable. In order to study the response of three wheat genotypes to different levels of waterlogging stres More

Waterlogging stress has negative effects on the growth and yield of wheat plants, which recognizes the defense mechanism of the plant against waterlogging, can be valuable. In order to study the response of three wheat genotypes to different levels of waterlogging stress, a factorial experiment was conducted based on a completely randomized design with five replications in Gharakhil (Ghaemshahr) Agricultural Station. The objective of this study was to investigate the effects of waterlogging (0, 7, 14 and 21 day) at tillering (ZG21) and stem elongation (ZG31) stages on the growth, total chlorophyll, proline, malondialdehyde (MDA), H2O2, activity of antioxidant enzymes, and enzymes of fermentation pathway in root of three wheat promising lines (N-93-19, N-93-9 and N-92-9). The results showed that waterlogging stress in both growth stages reduced the total chlorophyll and growth of all three genotypes, however, the highest contents of total chlorophyll and shoot and root dry weight were observed in N-92-9 genotype. Increased waterlogging stress increased the contents of proline, MDA, H2O2, activity of superoxide dismutase, catalase, alcohol dehydrogenase and lactate dehydrogenase and decreased peroxidase activity of root of genotypes in both growth stages compared to control treatments. The results of this experiment that N-92-9 genotype had better response than other two genotypes in all studied traits under waterlogging conditions and was introduced as a flood tolerant genotype. Manuscript profile

Open Access Article

12 - Comparison of Six Weeks of Weight Training with a Combination of Beta Hydroxy-Beta Methyl Butyrate and Creatine Supplement and Two Weeks of Non-Training on Creatine Kinase and Lactate Dehydrogenase in Male Powerlifting Athletes
Mojtaba Darvishkhadem Tahereh Bagherpour Nematollah Nemati

10.22034/ascij.2022.1926127.1241

The aim of this study was to compare six weeks of weight training with HMB and creatine supplementation and two weeks of non-training on creatine kinase and lactate dehydrogenase in male powerlifting athletes. Thirty-two male athletes aged 21 to 30 years were randomly d More

The aim of this study was to compare six weeks of weight training with HMB and creatine supplementation and two weeks of non-training on creatine kinase and lactate dehydrogenase in male powerlifting athletes. Thirty-two male athletes aged 21 to 30 years were randomly divided into four groups of 8 people creatine, HMB, HMB + Cr, and weight training. The training program was designed for 6 weeks and four sessions per week. HMB group 3 grams of supplement on training days and 1 gram on non-training days, creatine group on training days in 3 servings of 5 grams and 5 grams on non-training days, HMB + Cr group HMB supplement in 3 grams per day and Creatine was consumed in the same way as the creatine group. During the two weeks of training, the HMB group received 1 gr of the supplement daily, the creatine group received 5 gr of the supplement, and the HMB + Cr group received 1 gr of the HMB supplement daily and 5 grams of the creatine supplement. One-way analysis of variance with repeated measures was used to test the hypotheses. The test results showed that in the post-test between weight training groups and HMB (p = 0.000), creatine (p = 0.006) and HMB + Cr (p = 0.000) and in two weeks of inactivity, there was a significant difference in CK between the weight training group and HMB + Cr (p = 0.047). Also between weight training groups and HMB (p = 0.000), HMB + Cr (p = 0.000), creatine and HMB (p = 0.019), creatine and HMB + Cr (p = 0.001) in both Week of inactivity There was a significant difference in LDH between the weight training group and HMB + Cr (p = 0.004), creatine and HMB (p = 0.026), creatine and HMB + Cr (p = 0.001). It seems that 6 weeks of weight training with a combination of HMB and creatine supplement and 2 weeks of non-training can significantly reduce muscle injury indices in male powerlifting athletes. . Manuscript profile

Open Access Article

13 - The Effect of Eight Weeks of Low and High Volume HIIT Training on Lactate Response and Some Performance Indicators of Canadian Canoers
Farahnaz Amir Shaghaghi Fatemeh Karamjani

10.22034/ascij.2022.1941988.1319

The number of training sessions is an important factor in exercise training. Therefore, this study was aimed at investigating the effect of eight weeks of low and high volume HIIT training on lactate response and some performance indicators of Canadian canoe rowers. Six More

The number of training sessions is an important factor in exercise training. Therefore, this study was aimed at investigating the effect of eight weeks of low and high volume HIIT training on lactate response and some performance indicators of Canadian canoe rowers. Sixteen female rowers invited to the Canadian Canoe National Team Camp were randomly divided into two groups: low frequency interval training and high frequency interval training. Rowing ergometer and respiratory gas analyzer were used and at the same time, physiological variables of aerobic function including maximum oxygen consumption (VO2max), minimum speed to reach maximum oxygen consumption (vVo2max) and exhaustion time (Tmax) were measured. Intense intermittent training was performed in both groups in the form of 6 one-minute rowing sessions with 100% vVo2max and with an increase of two rounds per week. The difference was that the low-frequency group practiced 3 sessions per week and the high-frequency group practiced 6 sessions per week. Exercises were performed for eight weeks. Data were analyzed by paired t-test and independent t-test. Significance between variables was considered at the level of P≤0.05. The results showed a significant difference in lactate, VO2max, vVo2max and Tmax between pre-test and post-test of both high-volume periodic training and low-volume periodic training. However, no significant difference was observed in the post-test values between the two groups in any of the variables (P≤0.05). Due to the lack of significant differences between the two groups, the combination of these two methods at different times of a Canadian canoeing season seems to be helpful in preparing athletes. Manuscript profile

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14 - بررسی تأثیرمصرف کافئین بر پاسخ لاکتات خون متعاقب فعالیت های درمانده ساز در شنای قورباغه و پروانه شناگران
افشین رهبرقاضی معرفت سیاه کوهیان لطفعلی بلبلی

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15 - The Effect of Different Degrees of Dehydration on Plasma Creatine Kinase and Lactate Dehydrogenase Levels of Professional Wrestlers in Ahvaz
mehdi bostani reza sheikh

Despite the proving of the negative effects of dehydration, many athletes use of this strategy yet. The aim of this study was to investigate the effect of dehydration with different degrees on serum levels of enzymes biomarker muscle damage in professional wrestlers in More

Despite the proving of the negative effects of dehydration, many athletes use of this strategy yet. The aim of this study was to investigate the effect of dehydration with different degrees on serum levels of enzymes biomarker muscle damage in professional wrestlers in Ahvaz city. Among the professional wrestlers in Ahvaz city, after screening, 40 wrestlers were selected and randomly divided into three experimental groups and one control group. Experimental groups underwent dehydration at three levels of mild, moderate and severe. The plasma levels of Creatine Kinase (CK) and Lactate Dehydrogenase (LDH) were measured by enzyme kinetics method. for intragroup comparison and intergroup comparison, were used of the dependent t-test and analysis of variance (ANOVA) test respectively at a significance level of 0.05. The results showed that plasma CK levels increased significantly in the moderate and severe dehydration groups and LDH levels increased only in the severe dehydration group (P <0.05). Also, in the intergroup comparison of CK levels, it was observed that there was a significant difference between the severe dehydration group and other groups, as well as the moderate dehydration group and other groups (P <0.05). But no significant difference was observed between the moderate and severe dehydration groups (P <0.05). Also, the results of comparison between groups of LDH showed that only the difference between the severe dehydration group with other groups was significant (P <0.05) and the difference between the other groups was not significant (P <0.05). According to the results of this study, it is suggested that to prevent the possibility of muscle damage and consequently reduced the exercise performance, avoided the dehydration of more than 1% in one session and if necessary, dehydrate in more sessions and for longer. Manuscript profile

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16 - تاثیر کربوهیدرات با نمایه گلیسمی متفاوت بر غلظت گلوکز، لاکتات و عملکرد ورزشی متعاقب یک جلسه فعالیت استقامتی در زنان فعال شهر کرج
صبا آزادی مسعود معینی شبستری شهرام سهیلی

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17 - تاثیر مکمل دهی حاد ال-کارنیتین بر غلظت لاکتات در دوره های زمانی مختلف بازگشت به حالت اولیه در مردان فعال
مجتبی کاویانی مسعود معینی شبستری

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18 - تاثیر مکمل دهی تیامین پیروفسفات بر توان هوازی، تجمع لاکتات و حداکثر ضربان قلب طی فعالیت بیشینه در دختران فوتبالیست
پوران رستمی مسعود معینی شبستری

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19 - تاثیر مکمل سازی اسید چرب امگا- 3 بر شاخص التهابی CRP و شاخصهای آسیب سلولی سرمی پس از یک جلسه تمرین مقاومتی در هندبالیس تهای مرد جوان
شهلا حجت سیروان آتشک محمد امین گلی

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20 - Effect of oral drenching of calcium lactate in Prevention of hypocalcaemia in dairy cattle
آریا Badiei. . F Mosakhani مهدی Feyzi.

Subclinical and clinical hypocalcaemia (milk fever) are two major causes of economic loss in dairy cattle.At the onset of lactation, cows demand is 2.3gr calcium per kg of colostrum which is about nine times as muchcalcium as it is present in the entire plasma which cou More

Subclinical and clinical hypocalcaemia (milk fever) are two major causes of economic loss in dairy cattle.At the onset of lactation, cows demand is 2.3gr calcium per kg of colostrum which is about nine times as muchcalcium as it is present in the entire plasma which could result in milk fever. The economic losses of milk feverinclude: milk fever relapses&sbquo; downer cow&sbquo; dystocia and reproductive disease.In this study effect of oral administration of calcium lactate bolus evaluated in a diary farm. Twenty four out of48 pregnant cows randomly selected and treated with calcium bolus at calving or 12 hours after calving, the restwas considered as control group. Blood samples were collected from cows at 24 and 48 hours after calving andanalyzed for serum total calcium level in both control and treatment group. Statistical analysis showed signifcantdifferences between control and treatment groups (p<0.05). Manuscript profile

Open Access Article

21 - Determination of the diagnostic value of milk lactate dehydrogenase and alkaline phosphatase in bovine subclinical mastitis
عباس Kalantari SH Safi عباس Rahimi Foroshani

Currently somatic sell count(SCC) and bacterial culture is considered as the gold standard of detecting subclinical mastitis.however,the ebove-mentioned tests have a low diagnostic accuracy.therefore,for identification of infected animals,new biomarkers with high clinic More

Currently somatic sell count(SCC) and bacterial culture is considered as the gold standard of detecting subclinical mastitis.however,the ebove-mentioned tests have a low diagnostic accuracy.therefore,for identification of infected animals,new biomarkers with high clinical accuracy are needed.the objective of this study was to determine the diagnostic value of milk lactate dehydrogenase(LDH) and alkaline phophatase(ALP_ for the diagnisis of subclinical mastitis in dairy cows.the activities of these enzymes increase during mastitis,which make them to be the potential biomarkers for screening of mastitis.a total of 145 subclinical healthy cows were randomly selected .of these 77 cows were considered to be affected by sub clinical mastitis based on a SCC higher than 100*1000 cells/ml of milk and positive bacterial culture results of milk samples obtained from at least one of the quarters.enzymes activities were measured in blood serum and defatted milk(centrifuged at 5000-g for 15 min at 4)using commercial kits.diagnostic sensivity and specificity and cutoff points for each test were determined via receiver-operating characteristics curve.significant (p<0.001)increases in the mean and median activities of LDH and ALP were found in the milk samples collected from cows with subclinical mastitis.milk LDH had the most clinical accuracy with 94.8% sensitivity and 94.1% specificity at cutoff point of 109 U/L.the results of the present study showed that the measurement of LDH and ALP activities in milk samples could be used as reliable method for detection of bovine subclinical mastitis.     Manuscript profile

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