Investigating Resistance of Wild Mustard (Sinapis arvensis L.) ‎Populations to Tribenuron-Methyl Herbicide

Subject Areas : Journal of Crop Ecophysiology

‎ Mehdi  Afshari ¹ (Ph.D. Student of Weed Science of Ferdowsi University of Mashhad, Mashhad, Iran. )
Ali  Ghanbari ² (Associate Professor of Ferdowsi University of Mashhad, Mashhad, Iran.)
Mehdi  Rastgoo ³ (Associate Professor of Ferdowsi University of Mashhad, Mashhad, Iran.)
Javid  Gherekhloo ⁴ (Associate Professor of University of Gorgan, Gorgan, Iran)
Goodarz  Ahmadvand ⁵ (Associate Professor of Bu Ali Sina University of Hamedan, Hamedan, Iran.)

Received: 2016-02-28 Accepted : 2017-04-24 Published : 2017-05-22

Keywords: Mutation, DNA sequencing, Dose-response, ALS gene, PJET1.2/blunt Vector.‎,

Abstract :

Tribenuron-methyl is commonly used for post emergence control of broad leaf weeds in wheat fields. In order to survey suspicious resistant weeds in wheat fields to this herbicide thirty-eight fields of Kermanshah province were investigated during 2012- 2013. Seeds of suspected resistance of wild mustard were gathered and tested in a randomized complete blocks design experiment with three replications. First, for early detection of herbicide resistance, the suspected population was screened using discriminating dose of tribenuron-methyl. Determining of the resistance degree was conducted by whole plant bioassay tests using dose-response curves. The resistance mechanisms were assayed by molecular methods, especially using the ALS gene cloning by PJET1.2/blunt Vector. For susceptible populations, the concentration required for complete control was 10.4 g ai ha-1 tribenuron-methyl. Also, in screening tests 50% of populations as resistant populations were identified. According to the Beckie and Tardif, it was found that 57.8% of these population did have a very high degree of resistance, 31.5% with high resistance and 10/5% with low resistance degree. GR50 of the resistant weeds was also increased as compared to sensitive weed, which indicates resistance in this province, Thus to control the resistant populations Z15, this amount increased to 1309 g ai ha-1.The results of DNA sequencing showed that mutation by replacing proline amino acid at position Ala122 causes resistance based on target-site mutation.

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