List of Articles تریکوموناس

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  1 - Frequency of Tritrichomonas foetus Infection in cows of Isfahan
  , H. Tafazzoli Ali Sharifzadeh
  Tritrichomonas foetus is a protozoan parasite that has been traditionally identified as a cause of reproductive tract disease in cattle and may lead to Infertility and abortion. The most common and widely used method of T. foetus detection is direct micro More

  Tritrichomonas foetus is a protozoan parasite that has been traditionally identified as a cause of reproductive tract disease in cattle and may lead to Infertility and abortion. The most common and widely used method of T. foetus detection is direct microscopic examination. When specimens cannot be tested immediately after collection it is necessary to use transport medium or culture medium. This survey aimed to review the current status of Tritrichomonas foetus in Isfahan,Iran By Molecular Assay. Tritrichomonas foetus infection was investigated in 73 Bulls and 27 cows slaughtered in two abbattoir different. Mucus samples were obtained from the vagina and from the vagina and uterus, respectively, using a "screw-head scraper rod". Extraction of DNA from samples was carried out using a DNA extraction kit (Cinnagen, Iran), according to the manufacturer’s instructions. All samples were analyzed by Nested PCR technique using T. foetus-specific primers of Genus (TFR 1–2) and specie (TFR 3–4). All samples were analyzed by Nested PCR technique with the two sets of primers, respectively. Amplification products of 372 bp were observeded in 6 Bulls (8.2%) and 2 Cows (7.4%) isolates, with the genus specific primers and in 2 bulls also T. foetus yielded amplification products of the expected size (347 bp) showed. Other samples were related to non-T. foetus organisms. Therefore, based on the results of this survey, prevalence of Tritrichomonas genus and especially Tritrichomonas foetus in samples confirmed serious attention to screening test in bulls. Manuscript profile
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  2 - تعیین آلودگی به تریکومونیازیس در کبوترهای شهر تبریز
  میرهادی خیاط نوری حسین هاشم زاده فرهنگ وحید امین جوادی پریسا شهبازی
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  3 - Comparative study of the effect of aqueous and alcoholic extract of pomegranate peel on Trichomonas vaginalis in vitro
  Faranak Kalantare dezfuly Zohreh Momeni Mahdis Ebrahim Zadeh
  Objectives: Trichomoniasis is the most common non-viral sexually transmitted infection in the world. Metronidazole is currently used for the treatment of trichomoniasis, which has side effects. In recent years, the use of plants has been considered by researchers. This More

  Objectives: Trichomoniasis is the most common non-viral sexually transmitted infection in the world. Metronidazole is currently used for the treatment of trichomoniasis, which has side effects. In recent years, the use of plants has been considered by researchers. This study was conducted to investigate the anti-trichomonal activity of aqueous and alcoholic extract of pomegranate peel on Trichomonas vaginalis in vitro.Methods: In this experimental study, the extracts were prepared, and to evaluate the effect of aqueous and alcoholic extract of pomegranate peel on Trichomonas vaginalis parasites, the concentrations of 100, 200, 400, 800, 1600, and 3200 µg/ml were prepared. Metronidazole and TYM medium were considered positive and negative controls, respectively. Afterward, 105 live parasites were added to all wells, and all groups were kept at 37°C. Live parasites were counted at 24 and 48 h intervals by Trypan Blue using a neobar slide microscope (Hemocytometer). Also, the effect of metronidazole (0.5, 1, 2, 4, 8, and 10 µg/ml) was investigated as a drug control. Subsequently, the IC50 value for the above extract was calculated using GraphPad Prism 9. It should be noted that all steps of the experiment were performed in triplicate and the results were considered as average.Results: The IC50 of aqueous and alcoholic extract of pomegranate peel on Trichomonas vaginalis were calculated at 226.3, 109.9 μg/ml after 24h and 60.16 and 32.6 μg/ml after 48h, respectively. The IC50 of metronidazole was calculated at 0.28 and 0.1 μg/ml after 24h and 48h. The highest mortality rate (100%) was observed at a concentration of 3200 μg/ml after 24h of exposure.Conclusion: While the aqueous and alcoholic extract of pomegranate peel has an anti-Trichomonas effect, the anti-Trichomonas properties of the alcoholic extract are more than its aqueous extract. Further and more comprehensive studies are suggested to investigate this plant's constituents and the parasite's lethal effect in vitro and in vivo conditions.  Manuscript profile
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  4 - Survey of trichomoniasis infection in some of the Ornamental Birds in Babol city
  Jafar Hossienzadeh abdulhani shojaee
  Trichomonas gallinae, a flagellate parasite of animals vertebrates, is exclusively found in birds and there mostly in the pigeons and doves. Other birds may also become infected. Tricomonas gallinae is basically a parasite that has high levels of gastrointestinal tract More

  Trichomonas gallinae, a flagellate parasite of animals vertebrates, is exclusively found in birds and there mostly in the pigeons and doves. Other birds may also become infected. Tricomonas gallinae is basically a parasite that has high levels of gastrointestinal tract and respiration, a very high number of birds. This study was conducted in 1395 on 140 birds including 60 pigeons, 40 Budgerigars, 20 Canary, 10 pheasants, and 10 quails. In this examination the samples were gathered from mouth and larynx of those birds by soap and then, were inspected via wet spread slide method. Our results show that, among 140 birds, 35 pigeons (58.3%), 15 Budgerigars (25%), 8 canaries (13.3%), 2 pheasants (3.3%) were infected, but none of the 10 quails were infected with trichomonase. Contamination in hot season (75%) was higher than cold one (25%). Finally the results show that tricomonase contamination has high spread in babol city. That it is necessary to control this contamination strategies need to be applied. Manuscript profile
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  5 - Comparing the effect of metronidazole with pyocyanin pigment extracted from Pseudomonas aeruginosa on Trichomonas vaginalis in-vitro
  sara abdizadehjavazm zohreh momeni mona farhadi
  10.30495/jmw.2023.1962624.2025
  Background and Objectives: Pyocyanin is a blue pigment with oxidation and reduction potential and metabolically active, which has medicinal effects on eukaryotic and prokaryotic cells. Trichomonas vaginalis is the causative agent of trichomoniasis, the most important no More

  Background and Objectives: Pyocyanin is a blue pigment with oxidation and reduction potential and metabolically active, which has medicinal effects on eukaryotic and prokaryotic cells. Trichomonas vaginalis is the causative agent of trichomoniasis, the most important non-viral    sexually transmitted disease in the world. The aim of this study was to evaluate the effect of      pyocyanin extracted from Pseudomonas aeruginosa on Trichomonas vaginalis and PC12cell line. Materials & Methods: This study was carried out by an interventional  method. First, pyocyanin was extracted from Pseudomonas aeruginosa strain RTCC1474 with the help of chloroform. The relative purity of the pigment was determined by thin-layer chromatography, spectrophotometry UV-Vis and FTIR. Its effect in different concentrations were investigated on Trichomonas vaginalis and PC12 cell line. Results: Pyocyanin at a concentration of 10,000µg/ml in 24 hours and concentrations of 5,000 and 2,500µg/ml in 48 hours caused 100% inhibition of the parasite growth. Its IC50 (Inhibitory Concentration of 50%) level in 48 hours was 17.44µg/ml and the CC50 of this pigment on the cell line was 930 µg/ml, so pyocyanin is effective against the Trichomonas vaginalis and its toxicity on the cell line is 53 times higher than of the parasite (SI=53/32). Conclusion: Considering the inhibitory effect of pyocyanin on the growth of Trichomonas    vaginalis  based on the results, it is possible that with further research on the extraction and purification of this pigment from various isolates of Pseudomonas aeruginosa, and with additional tests in vitro and in vivo, a more accurate judgment regarding the antiparasitic power of this      pigment can be expressed.   Manuscript profile