Frequency of Tritrichomonas foetus Infection in cows of Isfahan

Subject Areas : Journal of Comparative Pathobiology

, H. Tafazzoli ¹ ( Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahre kord, Iran )
Ali Sharifzadeh ² ( Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. )

Received: 2021-11-01 Accepted : 2021-11-01 Published : 2020-11-21

Keywords: Tritrichomonasfoetus, Cattle, abbatoir, Nested PCR,

Abstract :

Tritrichomonas foetus is a protozoan parasite that has been traditionally identified as a cause of reproductive tract disease in cattle and may lead to Infertility and abortion. The most common and widely used method of T. foetus detection is direct microscopic examination. When specimens cannot be tested immediately after collection it is necessary to use transport medium or culture medium. This survey aimed to review the current status of Tritrichomonas foetus in Isfahan,Iran By Molecular Assay. Tritrichomonas foetus infection was investigated in 73 Bulls and 27 cows slaughtered in two abbattoir different. Mucus samples were obtained from the vagina and from the vagina and uterus, respectively, using a "screw-head scraper rod". Extraction of DNA from samples was carried out using a DNA extraction kit (Cinnagen, Iran), according to the manufacturer’s instructions. All samples were analyzed by Nested PCR technique using T. foetus-specific primers of Genus (TFR 1–2) and specie (TFR 3–4). All samples were analyzed by Nested PCR technique with the two sets of primers, respectively. Amplification products of 372 bp were observeded in 6 Bulls (8.2%) and 2 Cows (7.4%) isolates, with the genus specific primers and in 2 bulls also T. foetus yielded amplification products of the expected size (347 bp) showed. Other samples were related to non-T. foetus organisms. Therefore, based on the results of this survey, prevalence of Tritrichomonas genus and especially Tritrichomonas foetus in samples confirmed serious attention to screening test in bulls.

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