Frequency of Tritrichomonas foetus Infection in cows of Isfahan

Subject Areas : Journal of Comparative Pathobiology

, H. Tafazzoli ¹ , Ali Sharifzadeh ²

1 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahre kord, Iran
2 - Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Received: 2021-11-01 Accepted : 2021-11-01 Published : 2020-11-21

Keywords: Tritrichomonasfoetus, Cattle, abbatoir, Nested PCR,

Abstract :

Tritrichomonas foetus is a protozoan parasite that has been traditionally identified as a cause of reproductive tract disease in cattle and may lead to Infertility and abortion. The most common and widely used method of T. foetus detection is direct microscopic examination. When specimens cannot be tested immediately after collection it is necessary to use transport medium or culture medium. This survey aimed to review the current status of Tritrichomonas foetus in Isfahan,Iran By Molecular Assay. Tritrichomonas foetus infection was investigated in 73 Bulls and 27 cows slaughtered in two abbattoir different. Mucus samples were obtained from the vagina and from the vagina and uterus, respectively, using a "screw-head scraper rod". Extraction of DNA from samples was carried out using a DNA extraction kit (Cinnagen, Iran), according to the manufacturer’s instructions. All samples were analyzed by Nested PCR technique using T. foetus-specific primers of Genus (TFR 1–2) and specie (TFR 3–4). All samples were analyzed by Nested PCR technique with the two sets of primers, respectively. Amplification products of 372 bp were observeded in 6 Bulls (8.2%) and 2 Cows (7.4%) isolates, with the genus specific primers and in 2 bulls also T. foetus yielded amplification products of the expected size (347 bp) showed. Other samples were related to non-T. foetus organisms. Therefore, based on the results of this survey, prevalence of Tritrichomonas genus and especially Tritrichomonas foetus in samples confirmed serious attention to screening test in bulls.

References:

1. Frey C. Hemphill A. Scild M, Stunzi P .Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR.Parasit Res.2009. 104: 783–788.


2. Reinmann K. Muller N. Kuhnert P. Campero CM. Leitsch D. Hess M. Henning K. Fort M. Muller J. Gottstein B. Frey C. Tritrichomonas foetus isolates cats and cattle show minor genetic differences in unrelated loci ITS-2 and EF-1. Vet Parasit .2012.185: 138-144

 

3. Alan G. Sarah J. Parasitology an Integrated Approach. UK. John Wiley & Sons, Ltd.2012.


4. Owen R. John E. (2006). Tritrichomonas foetus. Vet Clin Food Anim.2006. 22: 595–611.


5. Noakes D. Parkinson T. Englamd G. Arthur’s Veterinary Reproduction and Obsthetrics. UK. Elsevier.2001.


6. Greenwell P.Younes M. Roughooputh S. Purification of DNases of Tritrichomonas foetus: evidence that these enzymes are glycoproteins. Inter J Parasit. 2008.38: 749–756.


7. Jesus A. Mendoza I. Susana P. High prevalence of Tritrichomonas foetus infection in Asturiana de la Montana beef cattle kept in extensive conditions in Northern Spain. Vet J.2012. 193: 146-151.


8. Oliveria M. Fitho A.Borges J. Soares L.Tritrichomonas Foetus in bulls in the state of parnambuco,Brazil.Rev Brast Med Vet.2016.38:449-453


9. Rae D. Crews J. (2006).Tritrichomonas foetus. Vet Clin Food Anim.2006. 22: 595–611.


10. Felleisen RS. Lambelet N. Bachmann P. Nicolet J. Muller N. Gottstein B. (1998).Detection ofTritrichomonas foetusby PCR and DNA enzyme immunoassay based on rRNA gene unit sequences.J Clin Microb. 1998.36: 513–519.


11. Grahn RA. BonDurant R H. VanHoosear KA. Walker RL. Lyons LA. (2005).An improved molecular assay for Tritrichomonas foetus.Vet Parasit.2005.127: 33–41.


12. Asadpour R. Salehi N. Jafari R. PCR Detection oh Trichomonad Species in the Semen of Bulls.Bulg J Vet Med. 2011.14: 215-220.


13. Buller N. Corney B. Bovine Trichomonosis. Australian New Zealand Standard Diagnostic Procedure. 2013 http://www.agriculture.gov.au/SiteCollectionDocuments/animal/ahl/ANZSDP-Bovine-trichomoniasis.pdf (assessed 1 August 2017).


14. Antonio P. Carlos M. Alfredo M. Marlene B. Identification of Tritrichomonas foetus pseudocysts in fresh preputial secretion samples from bulls. Vet Parasit. 2011.175: 1-8.


15. Campero C. Rodriguez C. Bolondi A. Cacciato C. Cobo E. Perez S. Odeon A. Cipolla A. Bondurant RH. Two-step (culture and PCR) diagnostic approach for differentiation of non-T.foetus trichomonads from genitalia of virgin beef bulls in Argentina. Vet Parasit.2003. 112: 167-175.


16. Cobo E. Favetto P. Lane V. Friend A. VanHooser K. Mitchell J. BonDurant R. Sensitivity and specificity of culture and PCR of smegma samples of bulls experimentally infected with Tritrichomonas foetus. Theriogen.2007. 68: 853-860.


17. Rae D. Crews J. Greiner E. Donovan G. Epidemiology of Tritrichomonas foetus in beef bull populations in Florida.Theriogen.2004. 61: 605-618.


18. Barbara S. Indumathi S. Andy S. Alfonso C. Renata I. Spatio-temporal epidemiology of Tritrichomonas foetus infection in Texas bulls based on state-wide diagnostic laboratory data.Vet Parasit. 2012.186: 450-455.


19. Evelyn M. Awoke G. Tiny H. Mkhevu M. Prevalence of Campylobacter foetus and Trichomonas foetus among cattle from Southern Africa.Afri. J. Biotec.2011.10:10313-10314