List of Articles مقاومت آنتی‌بیوتیکی

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Determination of phenotypic and genotypic pattern of antibiotic resistance in Escherichia coli isolates isolated from diabetic patients in Shahrekord
  fatemeh Khodaverdipour Amin Roozbehi
  Introduction: Preventing the spread of drug resistance is one of the most important issues in society. Escherichia coli is one of the most common bacterial agents isolated from the urinary tract and nosocomial infections. Treatment of infections due to it is difficult d More

  Introduction: Preventing the spread of drug resistance is one of the most important issues in society. Escherichia coli is one of the most common bacterial agents isolated from the urinary tract and nosocomial infections. Treatment of infections due to it is difficult due to the acquisition of resistance genes. This study aimed to investigate the phenotypic and genotypic pattern of antibiotic resistance in clinical uropathogenic strains isolated from diabetic patients. Materials and methods: A total of 51 E. coli isolates from urinary tract infection in diabetic patients obtained from clinical, were used in this study. Isolates were confirmed by chemical tests and molecular techniques based on tracking of the 16srRNA gene. Antimicrobial resistance assessment of isolates was done using molecular methods base on (qnrA, tet A, tet B, aac (3)IIa, sul1) and disk diffusion. Results: Most resistance r to ampicillin (66.66%) and the lowest resistance to Nitrofurantoin (1.96%) were reported. The frequency of tet A, tet B, qnr A, sul 1 and aac (3) IIa genes reported 68.62%, 64.7%, 29.41%, 39.21%, and 29.41% respectively. The statistical analysis shows a significant relationship between resistance to the antibiotic tetracycline and the gene tet A, tet B statistically. Conclusion: Early detection of resistant strains to select the most appropriate treatment options is essential to prevent the spread of resistance. It is suggested, as treatment for urinary tract infections is important, so to prevent drug resistance and treatment failure, it should be done according to the resistance pattern in the region. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Investigating the Antimicrobial Activity of Peperin Isolated from Black Pepper on Staphylococcus Aureus Isolated from the Throat and Nose
  Saeide Saeidi Mehdi Jahantigh Razvane Behzad Mehr Zahra Beigomi
  10.30495/qafj.2022.694996
  Due to the increasing resistance of pathogenic bacteria to common antibiotics, researchers are looking for antimicrobial agents of plant origin as alternative drugs. The aim of this study is to investigate the antimicrobial activity of peperin isolated from black pepper More

  Due to the increasing resistance of pathogenic bacteria to common antibiotics, researchers are looking for antimicrobial agents of plant origin as alternative drugs. The aim of this study is to investigate the antimicrobial activity of peperin isolated from black pepper on Staphylococcus aureus isolated from the nose and throat. Sampling was done from the pharynx and nose of the patients and 9 strains of Staphylococcus aureus were isolated and purified. The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MBC) in six concentrations were evaluated by dilution method in liquid medium on the determined bacteria. The results of this study showed that the lowest inhibitory concentration was equal to 8 μg/ml, which inhibited one strain, while the highest concentration was 512 μg/mg, which inhibited one strain. Staphylococcus aureus isolated the most It was resistant to trimethoprim (88.8%), ampicillin (77.7%), tetracycline (77.7%), erythromycin (66.6%) and ceftazidime (22.2%). The results of this study showed that piperine present in black pepper has a very strong inhibitory effect on Staphylococcus aureus bacteria isolated from the nose and throat. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Prevalence and antimicrobial susceptibility of Listeria monocytogenes in Koozeh cheeses of Urmia retails
  B. Abbasinejad مسلم Neyriz-Nagadehi Nagi Taher Talatappeh
     Koozeh cheese is of the local traditional cheeses of Azerbaijan and Kurdistan areas of Iran, and this cheese has high consumption among the people of these regions. Listeria monocytogenes is a food borne pathogen, and because of its growth at refrigeration More

     Koozeh cheese is of the local traditional cheeses of Azerbaijan and Kurdistan areas of Iran, and this cheese has high consumption among the people of these regions. Listeria monocytogenes is a food borne pathogen, and because of its growth at refrigeration temperatures has great concern in public health. In the present research, prevalence rate of L. monocytogenes in Koozeh cheeses of Urmia retails was surveyed. Moreover, antimicrobial susceptibility of the isolates was studied. One hundred samples of Koozeh cheese were collected randomly from Urmia retails in the spring of 2013. Samples were cultured in L-PALCAM Listeria selective enrichment broth with supplement. The enriched samples were transferred to PALCAM Listeria selective agar with antibiotics. For the identification of Listeria species, specific biochemical tests were performed. Antibiotic susceptibility of L. monocytogenes isolates was determined by disk diffusion method using penicillin G, chloramphenicol, ceftriaxone, erythromycin, tetracycline, and gentamicin. Among the 100 tested samples, L. monocytogenes was isolated from 3 of the samples. Antibiogram results showed that all L. monocytogenes isolates were susceptible to the tested antibiotics. However, tetracycline, penicillin and erythromycin were shown larger diameter of inhibition zone than the others. Although the prevalence rate of L. monocytogenes in Koozeh cheeses was low, its capability to growth in refrigerator conditions along with the pathogenicity of the bacterium to humans, hygienic production and distribution of these cheeses are recommended. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  4 - The Study on antibiotic resistance pattern of Cronobacter sakazakii strains isolated from infant formula and baby food types
  Samira abbasi bafetrat Mohammad Goli Hasan Momtaz
  Cronobacter sakazakii is one of the main pathogens that transmitted by feeding infants by contaminated infant formula and baby-food. This bacterium is one of the major causes of mortality, digestive diseases and meningitis in newborns. This study was performed in order More

  Cronobacter sakazakii is one of the main pathogens that transmitted by feeding infants by contaminated infant formula and baby-food. This bacterium is one of the major causes of mortality, digestive diseases and meningitis in newborns. This study was performed in order to detect microbiological and molecular contamination by Cronobacter sakazakii and study antibiotic resistance pattern of bacterial isolates. Two hundred samples of infant formula and baby food from deferent brands were collected and after microbial detection, bacteria’s isolates were detected, using PCR method. Then antibiotic resistance pattern of isolates were evaluated using disk diffusion method. Three samples from total 100 baby food (3%) and 5 samples from total 100 infant formula (5%) had Cronobacter sakazaki infection. A, B and C brands from infant formula and A, D brands from baby food were contaminated with Cronobacter sakazakii. B and C brands from infant formula (10% each of them) and A brand from baby food (8%) were infected with Cronobacter sakazakii. Isolates from Cronobacter sakazakii which isolate from infant formula and baby food demonstrated most Sensitivity to ciprofloxacin (20.38), meropenem (19.83) and imipenem (19.63) and most resistance to amoxicillin, ampicillin, tigecycline, ticarcillin, aztreonam and ceftazidime. Careful inspection on the raw material quality of infant formula and baby food as well as, exact supervision on production procedure, sanitation in addition to use natural antimicrobial can reduce Prevalence rate of this bacteria. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  5 - Prevalence of antibiotic resistance genes in Entrococcus faecalis isolated from meat in Shahrekord
  Hamed Karimian الهه Tajbakhsh ebrahim rahimi
    In the past two decades, Enterococci resistant to some antibiotics has gained widespread that it would cause complications in the treatment of infections caused by these bacteria. Since meat as a foodstuff consumed in Iran is common and easily contaminated wi More

    In the past two decades, Enterococci resistant to some antibiotics has gained widespread that it would cause complications in the treatment of infections caused by these bacteria. Since meat as a foodstuff consumed in Iran is common and easily contaminated with bacteria therefore, we decided to detect frequency of antibiotic resistance genes in Enterococcus faecalis isolated from meat in Shahrekord. This cross sectional study was conducted on 104 samples of meat in 2016 in Shahrekord. Isolation of bacteria using by biochemical methods and confirmed with detection of 16srDNA gene, and antibiotic resistance genes isolates using by Kirby-Bauer method and molecular method for detection of tetM, ant (2'''')-I, erm B and aac (6'')/aph (2'') genes. Most bacterial resistance to streptomycin (2/95%) and sensitivity to vancomycin (100%) were estimated. Prevalence of ermB gene reported 76.9% and prevalence of aac (6'')/aph (2'') gene reported 40% in E. faecalis isolated. The results indicate that is not resistance to vancomycin in E. faecalis isolated from meat in Shahrekord, but high resistance to Streptomycin, Cefotaxime, Meropenem, Erythromycin, Tetracycline were found also found that ermB gene in E. faecalis high prevalence of antibiotic-resistant gene is the most abundant strength. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  6 - Determining antibiotic resistance pattern in Enterobacter cloacae strains isolated from checken and sheep meat in Shahrekord city
  elahe barzam الهه تاج بخش Hassan Momtaz
  Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considere More

  Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considered as an opportunistic pathogen. In this research, the isolation of Enterobacter cloacae from chicken and sheep meat samples in Shahrekord in 2019 was done by microbial and molecular methods. Antibiotic resistance pattern was determined by disc diffusion method and microtitre plate method was used to check biofilm production. The ability to produce broad-spectrum β-lactamase enzymes was investigated through phenotypic and genotypic methods. Out of 384 examined samples, Enterobacter cloacae were identified in 25 samples (6.51%) which also confirmed in the presence of the hsp60 in molecular analysis. Among these, 18 samples were related to chicken meat (72%) and 7 samples (28%) were related to sheep meat. The highest antibiotic resistance to cotrimoxazole and cefotaxime was reported in 20 isolates (80%) and the lowest resistance to nitrofuranthein was reported in 15 isolates (23.8%). In microtiter method. 15 isolates (60%) showed strong biofilm reaction, 10 isolates (40%) showed moderate biofilm reaction. The present study indicates that ESBL-producing Enterobacter cloacae strains have a relatively high prevalence. The increase in the number of these strains is often caused by the irrational prescription of antibiotics, which requires the use of new antimicrobial agents,. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  7 - Prevalence of sul genes in E.coli isolated from urinary tract infectious in Shahrekord
  Mahnaz Shamaei Maryam Reisi Hossein Khodabandeh shahraki
  Urinary tract infections is one of the most common infectious diseases and E. coli is one of urinary tract infection the most important factor. The purpose of this investigation is prevalence of sul genes in E.coli isolated from urinary tract infectious in Shahrekord to More

  Urinary tract infections is one of the most common infectious diseases and E. coli is one of urinary tract infection the most important factor. The purpose of this investigation is prevalence of sul genes in E.coli isolated from urinary tract infectious in Shahrekord to form cross-sectional in 2013. Samples was prepared as sterile and in terms of urine tests, cultures and was studied. Investigation antimicrobial susceptibility was performed by disk diffusion method. As well as, for tracing Sul gene PCR reaction was performed in the presence of specific primers and the results was analyzed. In this study of 130 E. coli isolates studied 67 isolates (53/51%) resistance to co-trimoxazol was observed. The frequency of genes sul1, sul2 and sul3 was respectively 20/89%, 55/22% and 4/47%. In statistical analysis with chi-square test between to resistance sulfonamides and sul genes significant correlation was observed. The results showed that E. coli isolates are high resistant to sulfonamides that may be the indiscriminate use of these antibiotics. Manuscript profile