Prevalence of antibiotic resistance genes in Entrococcus faecalis isolated from meat in Shahrekord

Subject Areas : Food Microbial Contamination

Hamed Karimian ¹ ( Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord,Iran )
الهه Tajbakhsh ² ( Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran )
ebrahim rahimi ³ ( Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran )

Received: 2017-04-04 Accepted : 2017-06-05 Published : 2018-10-23

Keywords: Enterococcus, Enterococcus faecalis, meat, Antibiotic resistance genes,

Abstract :

  In the past two decades, Enterococci resistant to some antibiotics has gained widespread that it would cause complications in the treatment of infections caused by these bacteria. Since meat as a foodstuff consumed in Iran is common and easily contaminated with bacteria therefore, we decided to detect frequency of antibiotic resistance genes in Enterococcus faecalis isolated from meat in Shahrekord. This cross sectional study was conducted on 104 samples of meat in 2016 in Shahrekord. Isolation of bacteria using by biochemical methods and confirmed with detection of 16srDNA gene, and antibiotic resistance genes isolates using by Kirby-Bauer method and molecular method for detection of tetM, ant (2'''')-I, erm B and aac (6'')/aph (2'') genes. Most bacterial resistance to streptomycin (2/95%) and sensitivity to vancomycin (100%) were estimated. Prevalence of ermB gene reported 76.9% and prevalence of aac (6'')/aph (2'') gene reported 40% in E. faecalis isolated. The results indicate that is not resistance to vancomycin in E. faecalis isolated from meat in Shahrekord, but high resistance to Streptomycin, Cefotaxime, Meropenem, Erythromycin, Tetracycline were found also found that ermB gene in E. faecalis high prevalence of antibiotic-resistant gene is the most abundant strength.