Contamination of groundwater and surface water with nitrate has become a serious problem in many parts of the world. Adverse effects on aquatic ecosystems are one of the most important effects of high nitrate concentrations in aqueous solutions. Zero-valent iron (Fe 0) metal nanoparticles are of great interest for the purification of toxic compounds from water. The aim of this study was to use nanostructure coated with carbon-quantum dot (CQD-Fe0) to investigate their role in biodenitrification of Thiobacillus denitrificance. For this purpose, Fe0 nanoparticles were synthesized by the liquid phase reduction method and for biocompatibility, uniform distribution and non-agglomeration, Fe0 was coated with carbon quantum-dot. Characterization of nanoparticles determined by XRD, TEM, FESEM, FTIR and DLS. The FTIR spectrum confirmed the formation of CQD and Fe0 bonds. The average diameter of CQD-Fe0 nanoparticles was observed in the range of 29.31-38.32. According to the results, with increasing temperature, growth and bio-nitrification activity of Thiobacillus denitrificance microorganism increases.
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