Synthesis of copper oxide nanoparticles by chemical method and investigation of their antimicrobial properties

Subject Areas : microbiology

Mina Ramezani ^{1 *} , Asma Shamsai ² , Ramin Mohammadi-Aloucheh ³

1 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

Received: 2023-11-29 Published : 2025-01-08

Keywords: Copper oxide nanoparticles, Staphylococcus aureus, Escherichia coli, Fusarium solani,

Abstract :

Introduction: Copper oxide is one of the most important metal oxides that has unique properties in various technological applications, such as superconductors, and has been used as an antimicrobial agent against numerous bacterial species. In the present study, CuO Nps was synthesized using chemical methods and its antimicrobial activity was investigated. Materials and Methods: Copper oxide nanoparticles were synthesized using the microwave method, and XRD, EDX, SEM, FTIR, and UV-vis analyses were performed to investigate the physical and chemical properties. The antimicrobial and antifungal effects of copper oxide nanoparticles on pathogenic bacteria including Staphylococcus aureus, Escherichia coli, and Fusarium solani were evaluated using MIC and MBC tests and well diffusion assay. Results: The results showed that at a concentration of 20 mg/mL, copper oxide nanoparticles inhibited the growth of Staphylococcus aureus. Similarly, growth inhibition of Escherichia coli was observed at a concentration of 4 mg/mL of nanoparticles. Furthermore, Fusarium solani exhibited increased sensitivity to copper oxide nanoparticles at concentrations higher than 50 mg/mL, with a growth inhibition zone diameter of 18 mm at a concentration of 100 mg/mL. Discussion and Conclusion: Copper oxide nanoparticles can be introduced as a strong antimicrobial agent.