Effect of Inhibitor Carbonyl Cyanide 3-Chlorophenylhydra‌zone(CCCP) on the Minimum Inhibitory Concentration of Aminoglycoside and efflux pump activity determination in Acinetobacter baumannii Multiple- Drug Resistant isolates

Subject Areas : microbiology

Sahar  Honarmand Jahromy ¹ (Department of Microbiology, Islamic Azad university, Varamin- Pishva branch,Iran)
Jeyran  Fathi Rad ² (Department of Microbiology, Islamic Azad university, Varamin- Pishva branch,Iran)
Fatemeh  Noorbakhsh ³ (Department of Microbiology, Islamic Azad university, Varamin- Pishva branch,Iran)

Received: 2018-08-25 Accepted : 2018-09-06 Published : 2018-02-20

Keywords: Multiple drug resistance, Acineto‌bacter baumannii, efflux pumps activity,

Abstract :

Acietobacter baumanni is an important cause of nosocomial infections. This bacterium is resistant to many commonly-used antibiotics and multidrug resistance is often responsible for the failure of antibiotic therapy. Numerous mechanisms may lead to this resistance and the active efflux pumps is an important factor for mutidrug resistance in A. baumannii .Today aminoglycosides are important antibiotics used for the treatment of in A. baumannii serious infections.The aim of this study was investigation of efflux pupms activity for aminoglycoside resistance among clinical isolates of A. baumannii . 55 strains of Acinetobacter bumanni were isolated from clinical specimens of patients hospitalized in Milad hospital and identified by biochemical tests. Antibiotic resistance profile of strains to 11 antibiotics was determined by disc diffusion method according to CLSI standard and MDR strains were defined. Minimum inhibitory concentration (MIC) of amikacin and gentamicin was determined before and after treatment by efflux inhibitor carbonyl cyanide 3-chlorophenylhydrazone (CCCP). The highest resistance was to ceftazidim and cefotaxim (98.2%) and lowest resistance was to gentamicin(62%). Resistance to amikacin was 74.5%. The prevalence of MDR strains was 98% Among amikacin and gentamycin resistant isolates 4(9.3%) and 17(50%) strains reported as actively efflux pumps. One strain for both antibiotics had efflux pump activity. The efflux pump is more active for gentamicin tha amikacin among A. baumannii isolates.