Comparison and evaluation of the effect of disinfectants Microten, Savlon (Strymid-C), and benzalkonium chloride on Escherichia coli
Subject Areas : microbiology
faezeh matin
1
(
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
)
seyyedeh masumeh mirnurollahi
2
(
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
)
roudabeh behzadi andouhjerdi
3
(
Department of Genetics, Central Tehran Branch, Islamic Azad University, Tehran, Iran
)
Keywords: Disinfectant, Savlon, Benzalkonium, Microten, <i/>Escherichia coli<i>,
Abstract :
Introduction: Due to the importance of disinfection and prevention of diseases caused by Escherichia coli, sometimes the companies producing these disinfectants have exaggerated and it causes the employees of health centers and hospitals to use these products as sterilizers to sterilize surfaces and places without conducting tests. If these products do not have the recommended efficiency and effectiveness mentioned in the instructions of the manufacturer, it will cause irreparable losses.Aim: This study was carried out in 1400 with the aim of evaluating the effect of three disinfectants Microten, Benzalkonium Chloride, and Savlon with recommended dilutions on Escherichia coli isolates from general hospital wards. Materials and methods: In this study, 50 samples were collected from the general departments of the hospital, and E.coli were analyzed using biochemical and molecular methods. E.coli (G4S45) was used as a control. Bacteria were cultured using the pour plate method using the pharma dilution method according to the American USP standard. Finally, the effect of the mentioned disinfectants on E.coli was investigated.Results: 19 samples were positive for the presence of E.coli and three disinfectants were in 5, 10, and 15 minutes after incubation, and performing the pour plate method caused a 100% lack of growth.Discussion: the efficiency of the three tested disinfectants was 100% effective according to the manufacturer's instructions.
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