The effects of cation-anion balance and dietary protein levels on performance, biochemical and haematological parameters in broiler exposed to heat stress
Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)
Keywords: Protein, broiler chickens, heat stress, Biochemical parameters, Electrolyte,
Abstract :
This experiment was carried out to determine the effects of different levels of protein and electrolyte balance (DEB) on performance, biochemical and haematological parameters in Ross (308) broiler exposed to heat stress. In this experiment, 486, one-day old broilers were used in a completely randomized design with a 3×3 factorial arrangement with 3 replicate for a treatment. The diets were formulated according to NRC (1994) recommendation with protein levels (100, 90 and 80% of NRC recommendations) and electrolyte balance (200, 260 and 320 meq/kg). The birds were exposed to heat stress (34±30C) for 8 hours/day (10:00 to 18:00). The results of experiment indicated that body weight gain was significantly different in starter and in broilers fed containing 260 meq/kg DEB and protein (90%) were significantly higher than those fed other levels of DEB and protein, in all periods (starter, grower and total period). In comparison to 200 DEB, the level of 260 DEB increased body weight gain and feed intake in all periods (p<0.05). Dietary protein levels had no significant effect on body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR). Dietary DEB and protein had no significant effect on cholesterol, triglyceride, total protein, albumin, sodium and potassium. The experimental groups did not affect on Haemoglobolin, Haematocrit, hetrophyl, lymphocyte, monocyt and White blood cells counts. The concentration of chlorine was decreased by increasing DEB (p<0.05). As a result, using of DEB 260 meq/kg and medium protein level (90% NRC) for broilers exposed to heat stress is recommended.
1- نوبخت، ع. (1386): اثر توازن الکترولیت‌های جیره بر عملکرد، کیفیت تخم‌مرغ و فراسنجه‌های بیوشیمیائی و هماتولوژیکی مرغ‌های تخم‌گذار در شرایط معمول حرارتی و حرارت بالا در مراحل اول و آخر تخم‌گذاری. پایان نامه دکتری. واحد علوم و تحقیقات تهران.
2- نظیفی، س. (1379): هماتولوژی و بیوشیمی بالینی پرندگان (تالیف). انتشارات دانشگاه شیراز.
3- Adekunmisi, A. A., and K. R. Robbins., (1987). Effects of dietary crude protein, electrolyte balance, and photoperiod on growth of broiler chickens. Poult. Sci. 66:299-305.
4- Ahmad, T., Sarwar. M., Mahr-Un. Nisa, Ahsan-ul- haq and Zia-Ul-Hasan., (2005): Influence of varying sources of dietary electrolytes on the performance of broilers reared in high temperature environment. Anim. Feed. Sci. and Tech. 20: 227-298.
5- Bartov, I., S. Bornstein, and B. Lipstein., (1974): Effect of calorie to protein ratio on the degree of fatnees in broiler fed on practical diet. Br .Poult. Sci. 15: 107-117.
6- Belay, T. and R. G. Teeter. (1993). Broiler water balance and thermobalance during thermo neutral and high ambient temperature exposure. Poult. Sci. 72: 16- 24.
7- Benton, C. E., D. Balnave and J. Brake., (1998): Review: the use of dietary minerals during heat stress in broilers. The professional Anim. Scientist. 14: 193- 196.
8- Borges, S. A. (1997). Suplementac¸a˜o de cloreto de pota´ssio e bicarbonato de so´d io para frangos de corte durante o vera˜o. Dissertac¸a˜o de mestrado. UNESP, Jaboticabal, Brazil.
9- Borges, S. A., (2001): Balanco electrolitico e sua interrelacao com o equilibrio acidobase em frangos de corte submetidos a estresse calorico. Jaboticabal. Tese (Doutorado em Zootecnia) Curso de Posgraduacao em Zootecnia, Universidade Estadual Paulista. PP. 97.
10- Borges, S. A., A. V. Fischer da Silva, A J. Ariki, D. M. Hooge and K. R. Cummings., (2003a): Dietary electrolyte balance for broiler chickens under moderately high ambient temperatures and relative humidities. Poult. Sci. 82: 301- 308.
11- Borges, S. A., A. V. Fischer da Silva, A J. Ariki, D. M. Hooge and K. R. Cummings. 2003b. Dietary electrolyte balance for broiler chickens exposed to thermoneutral or heat stress environments. Poult. Sci. 82: 428- 435.
12- Borges, s. A., A. V. Fischer da Silva, A. D. Meira, T. Moura, A. Maioka, and A. Ostrensky. (2004b): Electrolyte balance in broiler growing diets. Intl. J. Poult. Sci. 3: 623-628.
13- Borges, S. A., A. V. Fischer da Silva, A. Majorka, D. M. Hooge and K. R. Cummings. (2004a): Effects of diet and cyclic daily heat stress on electrolyte, nitrogen and water intake, excretion and retention by colostomized male broiler chickens. Intl. J. Poult. Sci. 3: 313- 321.
14- Borges, S. A., A. V. Fischer da Silva, J. Ariki, D. M. Hooge and K. R. Cummings. (2003a): Dietary electrolyte balance for broiler chickens exposed to thermoneutral or heat stress environments. Poult. Sci. 82: 428- 435.
15- Borges, S. A., J. Ariki, E. Santin, F. da Silva and A. Maioka., (1999). Balancoeletrolitico em dieta preinicial de frangos de corte durante o vera Revista Brasileira de Ciencia Avicola. 1: 175-179.
16- Borges, S.A., Maiorka, A., Laurentiz, A.C., Fischer Da Silva, A.V., Santin, E. and Ariki, J., (2002): Electrolyte balance in broiler chicks during the first week of age. Revista Brasileira de Ciencia Avicola. 4: 149-153.
17- Darre, M. J., P. C, Harrison., (1987): Heart rate, Blood pressure, cardiac output, and total peripheral resistance of single comb white leghorn hens during an acutee exposure to 35⁰C ambient temperature.Poult. Sci. 66: 541-547.
18- Fangyan, D,A. Higginbotham, and D.White., (2000): Food intake,energy balance and serum leptin concentrations in rats fed low-protein diets.J of Nutr. 130:514-521.
19- Fixter, M., D, Balnave and R. J. Johnson., (1987): The influence of dietary electrolyte balance on broiler growth at high temperatures. In: proc. Foundation Symp. Univ. of Sydney, Australia. Pp. 34-48.
20- Gross, W.B. and H.S. Siegel., (1983): Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Diseases. 27: 972-979.
21- Harper, H. A., V. W. Rodwell, and P. A. Mayes., (1977): Review of Physiological chemistry. Lange Medical publication0 Los Altos. CA.
22- Johnson, R. and K. Ajeewa., (1985): The effects of dietary minerals and electrolytes on the growth and physiology of the young chick. J. Nutr. 115: 1680-1690.
23- Lacroix, R., D.R. Keeney, and L.M. Welsh., (1970): Potentiometric titration of chloride in plant tissue extracts using the chloride ion electrode. Commun. Soil Sci. and Plant Anal. 1:1-6.
24- Martinez- Amezcua, C., J. L. Laparra-vega, E. Avila-Gonzalez, U. Cortes-Poblano, and M. T. Kidd., (1998): Dietary lysine and electrolyte balance do not interact to affect broiler performance. J. Appl. Poult. Res. 7: 313-319.
25- Maxwell, M. H. and G. W. Robertson., (1998): The avian heterophil lcukocyte: A Review.World’s. Poult. Sci. J. 54: 155-178.
26- Murakami, A. E., J. R. G. Franco, E. N. Martins, E. O. Oviedo Rondon, and M. S. Pereira., (2001): Effect of electrolyte balance in low protein diets on broiler performance and tibial dyschondroplasia incidence. J. Appl. Poult. Res. 12:207- 216.
27- Murakami, A. E., J. R. G. Franco, E. N. Martins, E. O. Oviedo Rondon, M.I. Sakamoto, and M. S. Pereira., (2003): Effect of electrolyte balance in low protein diets on broiler performance and tibial dyschondroplasia incidence. J. Appl. Poult. Res. 12: 207-216.
28- Nassiri Moghaddam, H., H. Janmohammadi, and H. Jahanian Najafabadi., (2005): The effects of dietary electolyte balance on growth, tibia ash and some blood serum electrolytes in young pullets. Inter. J. of. Poult. Sci. 4(7): 493-496.
29- National Research Council (NRC), (1994): Nutrient Requirements of Poultry. 9th rev. ed. National Academy Press, Washington, DC.
30- Nizamettin, S. H., Akyurek. H. Ersin. and A. Agma., (2005): Assessment the impacts of dietary electrolyte balance levels on laying performance of commercial white layers. Pak. J., Nut. 4(6): 423-427.
31- Ozbey, O., N. Yildez, M. H. Aysondu and O. Ozman., (2004): The effect of High temperatures on blood serum Parameters and the egg productivity characteristics of Japanese quails (Coturinx japanica). Int. J. poult. Sci. 485- 489.Academic Press, Washington, DC
32- Ozbey, O., N. Yildiz, M. H. Aysondu and O. Ozman., (2004): The effects of High temperature on blood serum parameters and the egg productivity characteristics of Japanese quails (Coturnix coturnix japonica). Int. J. Poult. Sci. 485-489.
33- Rezaei, M., H. Nassiri_Mogaddam, J. Pourreza, And H. Kermanshahi., (2004): The effect of dietary protein and lysine levels on broiler performance, carcass characteristics and nitrogen excretion. J. Poult. Sci. 3(2): 148-152. Ueno, T., Y. Miyazono and T. Komiyama., (1978): Breed differences of feed and water consumption and some physiological traits of chickens reared under different environmental temperatures. Japan. Poult. Sci. Ibaraki. Japan. 15: 189-194.
34- Sahin, K., O. Kucuk. N. sahin and M. Sari., (2001): Effect of vitamin C and vitamin E on lipid peroxitation status, some serum hormone, meabolite, and mineral concentrtins of Japanese quails reared under heat stress (34Ċ). Int. J. Vitam. Nutr. Res. 71: 27-31.
35- SAS Institute., (1985): SAS User's guide: Statistics, Version 5. SAS (Statistical Analysis System) Institute Inc., California, USA.
36- Senkoylu, N., and M. Altinsoy., (1999): The physiological views of stress. J. Farm. Istanbul. Turkey. 187: 37-39.
37- Soliman, K. F. A. and T. M. Huston., (1974): Effect of dietary protein and fat on the plasma cholestrerol and packed cell volume of chickens exposed to different environmental temperature. Poult. Sci. North Dunlap. Savoy. 53: 161-166.
38- Soliman, K. F. A. and T. M.Huston., (1974): Effect of dietary protein and fat on the plasma cholestrol and packed cell volum of chichens exposed to different environmental temperature. Poult. Sci. North Dunlap. Savoy. 53: 161- 166.
39- Surisdiarto, N. and D. Farrell., (1991): The relationship between dietary crude protein and dietary lysine requirement by broiler chicks on diets with and without the "ideal" amino acid balance. Poult. Sci. 70:830-836.
40- Takahashi, K. and Y. Akiba., (2002): Effects of oral administration of Diakur Tm (a glucose and electrolyte additive) on growth and some physiological esponses in broilers reared in a high temperature environment. Asian Aust. J. Anim. Sci. 15: 1341-1347.
41- Ueno, T., Y. Miyazono and T. Komiyama., (1978): Breed differencecs of feed and water consumption and some physiological traits of chickns reared under different environmental temperatures. japan. poult. Sci. Ibaraki. Japan. 15: 189-194.
42- Vecerek, M., S. Strakova, P. Sunchy and E. Voslarova., (2002): Influence of high environmental temperature on production and haematological indexes in broiler chickens. Czech J. Anim. Sci. 47: 176-182.
43- Zhou, W. T., M. Fujita and S. Yamamoto., (1999): Thermoregulatory responses and blood viscosity in dehydrated heat exposed broilers (Gallus domesticus). J. Therm. Biol. 24: 185-192.
44- Zulkifli, L., E. A. Dunnington, W. B. Gross and P. B. Siegel., (1995): Inhibition of adrenal steroidogenesis, food restriction and acclimation to high ambient temperatures in chickens. Brit. Poult. Sci.35: 417- 426.
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