Comparative study of Serum Amyloid A in Cows Affected by Subclinical Mastitis due to E.coli and Staphylococcus aureus
Subject Areas : Journal of Veterinary Clinical and Laboratory Research(JLACSR)
ommolbanin ghasemian
1
*
,
Seyedeh Zeinab Peighambarzadeh 1 Seyedeh Zeinab Peighambarzadeh 1
2
,
mehrad birgani
3
1 -
2 - 1- گروه دامپزشکی، واحد شوشتر، دانشگاه آزاد اسلامی، شوشتر، ایران .
3 - 1. Department of Veterinary, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran.
Keywords: : Escherichia coli, Inflamatory markers, Mastitis, Staphylococcus aureus,
Abstract :
This study aimed to evaluate the diagnostic value of Serum Amyloid A (SAA), in the early detection of subclinical mastitis in cows infected by Escherichia coli (E.coli) and s taphylococcus aureus infections. This cross-sectional analytical study, conducted in 2024 in laboratory, Isfahan, Iran, evaluated inflammatory markers in 79 dairy cows with clinical and subclinical mastitis. The cows divided into three groups: healthy cows, cows with subclinical mastitis, and cows with clinical mastitis. These groups were then evaluated for Serum Amyloid A (SAA). The diagnostic value of the inflammatory markers was determined by calculating the areas under the curves (AUCs) of the ROC curves. Generally, among the patients with a positive culture test (57%), 19% were found to be infected with E. coli, 22.8% with Streptococcus uberis, and 15.2% (12 cases) with Staphylococcus aureus. A strong correlation was observed between the mean SCC and the values of SAA (P<0.005). The sensitivity and specificity of SCC (0.98), SAA (0.90) were high for diagnosing mastitis in cows. The present study revealed that mastitis in dairy cows is associated with an increase in inflammatory cytokines such as amyloid A. The study suggests that variations in this biomarker could be utilized for disease diagnosis
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