Introduction & Objective: Various peptides are involved in food intake regulation in the bird and mammalian brain. Melanocortin and corticotropin play an important role in food intake control. On the other hand, phoenixin-14 increases food intake. This study aimed t
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Introduction & Objective: Various peptides are involved in food intake regulation in the bird and mammalian brain. Melanocortin and corticotropin play an important role in food intake control. On the other hand, phoenixin-14 increases food intake. This study aimed to investigate the synergistic effects of corticotropin and melanocortin with a sub-effective dose of phoenix -14 on food intake in neonatal chickens.Materials and Methods: A total of one hundred and ninety-two neonatal chicks were randomly divided into three experimental groups. Each experiment had a control group and three treatment groups (n=12 in each group). In all experiments, 3-hours food-deprived birds received intracerebroventricular ‎injections of either control diluent or drug solution. Then the birds had ad libitum access to the ‎food and fresh water, and then cumulative food intake (gr) was measured based on the ‎percentage of the body. In the first experiment, control solution, phoenixin-14 (0.8 nmol), Astressin-B (CRF1/CRF2 receptor antagonist; 30 µg), and phoenixin-14 + Astressin-B were injected. ‎ In the second experiment, control solution, phoenixin-14, Astressin-2B (CRF2 receptor antagonist; 30 µg), and phoenixin-14 + Astressin-2B were injected. In the third experiment, control solution, phoenixin-14 (0.8 nmol), SHU9119 (MC3/MC4 receptor antagonist; 0.5 nmol), and phoenixin-14 + SHU9119 were injected. In the fourth experiment, control solution, phoenixin-14 (0.8 nmol), MCL0020 (MC4 receptor antagonist; 0.5 nmol), and phoenixin-14 + MCL0020 were injected.Results: The results showed that co-inject ion of phoenixin-14 + Astressin-B and phoenixin-14 + Astressin-2B significantly increased food intake(p<0.05), while co-injection of phoenixin-14 + SHU9119 and phoenixin-14 + MCL0020 had no effect(p>0.05).Conclusion: According to the results of the present study, there is probably a synergistic effect between phoenixin-14 and corticotropin system on food intake control of neonatal chicks.
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