Number 3 Volume 16

Open Access Article

1 - Effect of Postnatal Administration of Vitamin E on Autistic-like Behavior in a Rat Model of Autism
Azin Sadat Seyed Atashi Hamid Sepehri

Background & aim: autism spectrum disorders (ASD) represent a heterogeneous group of neurodevelopmental syndromes that are clinically characterized by deficits in social interactionand repetitive behaviors . The pathogenesis of ASD is complex and not fully disclosed More

Background & aim: autism spectrum disorders (ASD) represent a heterogeneous group of neurodevelopmental syndromes that are clinically characterized by deficits in social interactionand repetitive behaviors . The pathogenesis of ASD is complex and not fully disclosed. Vitamin E is a fat-soluble vitamin that has strong antioxidant activity. Previous studies have reported decreased plasma levels of vitamin E in autism spectrum patients and beneift effect of prenatal treatment . The present study was conducted to investigate the effect of two week postnatal treatment of vitamin E on autism-like behavior in rats exposed to VPA. Materials and methods: pregnant rats were divided into two groups from the 14th day of pregnancy, one group received only normal food and water, and the other group was injected with valproic sodium on the 14th day of pregnancy. The offspring were tested for repetitive behaviors and cognitive ability with a T-maze task and social interaction with a play behavior task on 44st of Postnatal daysResults: Administration of vitamin E in mice treated with VPA increased cognitive flexibility compared to VPA treatment alone. Vitamin E treatment of mice treated with VPA led to the improved social behavior of pinning with strange mice . Administration of vitamin E in VPA-treated mice did not improve any of the social behaviors including sniffing, running, and grooming compared to valproic acid treatment aloneConclusion: The findings of the present study showed that vitamin E treatment improves cognitive flexibility and to some extent social behaviors in autistic rats. Manuscript profile

Open Access Article

2 - The effect of two training methods (continuous and periodic) and ginger supplementation on the balance of apoptosis and oxidation pathway in heart tissue of breast cancer model rats
Mohammad Hossein Bargahi parvin farzanegi Farzad Feli Kohikheili

Introduction and Objective: Today, breast cancer is one of the most common malignant diseases in women and its incidence is increasing, the aim of the present research was the effect of continuous and intermittent training methods with ginger supplement on the balance o More

Introduction and Objective: Today, breast cancer is one of the most common malignant diseases in women and its incidence is increasing, the aim of the present research was the effect of continuous and intermittent training methods with ginger supplement on the balance of apoptosis and oxidation pathway in heart tissue of breast cancer model rats.Methodology: In this experimental research, 56 mice were randomly divided into 8 groups: 1) control, 2) cancer, 3) cancer-saline, 4) cancer-supplement, 5) cancer-periodic, 6) cancer-continuous, 7) cancer-periodic-supplement and 8) cancer-continuous-supplement group were placed. The continuous program of the first week with a speed of 15 m/min for a duration of five minutes on the treadmill, which increased the speed by 1-2 m/min every week and increased the running time by 1-2 minutes. Intermittent training includes six sets of 2.5 minutes, with four minutes of rest between each set. Results: The results showed that the induction of cancer increased the expression of BAX and MDA in the heart tissue of rats and significantly decreased the expression of BCL-2 and SOD, each of the interventions (continuous exercise, ginger and the combination of continuous and intermittent exercise with ginger supplement) The cardiac expression of BAX and MDA decreased and the expression of BCL-2 and SOD increased significantly.Conclusion: The results confirm the role of continuous and periodic exercise with ginger supplement in improving the apoptotic and oxidative indices of heart tissue in breast cancer mice, which may have a protective effect on heart tissue. Manuscript profile

Open Access Article

3 - Investigating the effect of Eclipta prostrata hydroalcoholic extract on motor activity, avoidance memory and oxidative stress in an animal model of Parkinson's disease in adult male rats
shahrbano alamirostami Maryam Rafieirad

Background and Aims: Parkinson's disease is associated with motor and cognitive disorders, so we investigated the administration of Eclipta prostrata hydroalcoholic extract on learning, memory, motor activity and oxidative stress in Parkinson's model.Materials and metho More

Background and Aims: Parkinson's disease is associated with motor and cognitive disorders, so we investigated the administration of Eclipta prostrata hydroalcoholic extract on learning, memory, motor activity and oxidative stress in Parkinson's model.Materials and methods: 50 adult Wistar rats were divided into five groups: control group, parkinsonian group and three groups treated with eucalyptus perstrata extract in three different concentrations of 50, 100 and 200 mg/kg. Induction of Parkinson's model was done by injecting 6-hydroxydopamine (6-OHDA). One day after the last gavage, motor tests were done. The shuttle box test was used to evaluate learning and avoidance memory. Oxidative stress was evaluated by malondialdehyde, glutathione peroxidase and thiol content.Results: 7 days after the lesion in the MFB, after the administration of apomorphine, the rats turned 360 degrees in the right direction at a rate of more than 10 revolutions per minute. In the movement tests of the parkinsonian group, maintaining balance in rotarod (p<0.001), catalepsy (p<0.001), muscle stiffness (p<0.001), stride length (p<0.001) and avoidance memory (p<0.001) showed a significant difference to the control group. Also, Eclipta prostrata extract significantly improved all kinds of movement disorders caused by Parkinson's disease, and in doses of 50, 100, and 200, it improved memory in Parkinsonian rats (p<0.001). Also, the extract significantly increased the amount of thiol (p<0.001). and glutathione peroxidase (p<0.001) and decreased MDA in hippocampus and striatum tissue (p<0.001).Conclusion: In general, we showed that the hydroalcoholic extract of Eclipta prostrata administered in the animal model of Parkinson's has a favorable effect on memory, learning, motor activity and oxidative stress of the brain. Manuscript profile

Open Access Article

4 - Central Effects of Phoenixin and Muscimol on Pentylenetetrazol-Induced Seizures during Various Stages of the Estrous Cycle among Rats
Aliakbar Azadi Morteza Zendehdel Jahangir Kaboutari Negar Panahi Ahmad Asghari

Background & Aim: Catamenial epilepsy is a special form of epilepsy in women whom seizure aggravation is arranged with menstrual cycle that may affect up to 70 % of epileptic women. According to earlier studies, the role of neuropeptide Phoenixin (PNX) in the r More

Background & Aim: Catamenial epilepsy is a special form of epilepsy in women whom seizure aggravation is arranged with menstrual cycle that may affect up to 70 % of epileptic women. According to earlier studies, the role of neuropeptide Phoenixin (PNX) in the reproductive system has been observed and its antiepileptic effects have been proven. It also appears that the GABAergic system plays an important role in inhibition of seizures. Therefore, the aim of this study was to investigate the central effects of PNX and muscimol (GABA-A receptor agonist) on Pentylenetetrazol (PNT)-induced seizures during various stages of the estrous cycle among rats.Materials and Methods: In this study, 120 adult female rats were randomly divided into five groups, including intracerebroventricular (ICV) injection of normal saline, PNX (5 μg), PNX (10 μg), muscimol (0.1 μg)+ PNX (5 μg) and muscimol (0.1 μg)+ PNX (10 μg), in proestrus, estrus, metestrus, and diestrus. After ICV injections, acute epilepsy was induced by intraperitoneal (IP) injection of 80 mg/kg of pentylentetrazole. Initiation time of myoclonic seizures (ITMS) and initiation time of tonic–clonic seizures (ITTS) were monitored and recorded for 30 min.Results: The results of the present study showed that PNX alone increased both ITMS and ITTS in all phases of estrus (p<0.05). Furthermore, the injection of PNX with muscimol significantly reinforced the effects of the PNX on ITMS and ITTS in all estrus stages (p<0.05).Conclusion: The results showed that the antiepileptic activity of PNX was probably mediated by GABA-A receptors. Manuscript profile

Open Access Article

5 - the effect of galbanic acid on the prevention of intra-abdominal adhesion after surgery in rat
Melika Montazeri Firoozeh Saghaei

Background and Aim: Abdominal adhesions are a common complication after abdominal surgery that can cause intestinal obstructions, pelvic pain, or infertility. This study was conducted with the aim of investigating the effect of galbanic acid on preventing the formation More

Background and Aim: Abdominal adhesions are a common complication after abdominal surgery that can cause intestinal obstructions, pelvic pain, or infertility. This study was conducted with the aim of investigating the effect of galbanic acid on preventing the formation of intra-abdominal adhesions after surgery in rats.Material & Methods: 40 male Wistar rats were randomly divided into 4 groups of 10: after abdominal surgery and induction of adhesion, in the control group: 1cc of distilled water, in the galbanic acid group (GA5):  galbanic acid with a dose of 5mg/kg, in the galbanic acid group (Ga10): galbanic acid with a dose of 10 mg/kg and in the tween group: 0.5 cc of tween (solvent of galbanic acid) was prescribed intraperitoneally for 10 days. After the end of the course, the rats were anesthetized, and the open abdomen and adhesive bands were observed and graded and analyzed with spss software and chi-square test. A tissue sample was removed from the adhesion site and after staining, it was observed under a microscope.Results: Galbanic acid in doses of 5 and 10 mg/kg significantly reduced adhesion compared to the control and tween groups. Histopathological studies show the beneficial effect of galbanic acid by reducing collagen fibers and fibrocytes.Conclusion: Galbanic acid with anti-fibrin, antioxidant and anti-inflammatory properties in doses of 5 and 10 mg/kg can prevent intra-abdominal adhesions in rats. Manuscript profile

Open Access Article

6 - Investigating the effects of injecting intravenous anesthetics Propofol, sodium thiopental, fentanyl during implantation on the rate of abnormalities in rats.
Shiva Shojaifard Mohamad Reza Jamshidi Elham Hosseini Somayeh Abdollahi sabet مهدی رهنما

Background & Aim: Background and purpose: Anesthesia is very important for assisted reproductive technology to provide minimal adverse effects on the oocyte. Defects in the development of the embryo or abnormality in its growth phenomenon lead to IVF failure in most More

Background & Aim: Background and purpose: Anesthesia is very important for assisted reproductive technology to provide minimal adverse effects on the oocyte. Defects in the development of the embryo or abnormality in its growth phenomenon lead to IVF failure in most cases. The number of abnormalities in laboratory rats is RAT.Materials & methods: Propofol, Nestonal, and Fentanyl were used for single and combined anesthesia. The sham group was studied to control injection stress by injecting distilled water and the control group without receiving drugs. In the next step, the fertility conditions of the mice were provided. The effectiveness of the drugs is relatively similar to the selected doses, and after counting, cesarean surgery is performed on each fetus, and after the placenta is carefully separated from the fetus, both are carefully weighed, measured, and recorded.Results: The difference in the number of births was not observed, but the weight of the placenta and the weight of the fetus were investigated as indicators determining the effect of drugs on the fetus, which can be a symbol of fetal abnormalities. The results showed that the drugs used had no effect on conception and its success rate, but they were effective on fetal growth, and propofol had the least effect on abnormalities, and there was a significant difference in placenta and fetal weight between this group and the other group. The birth weight of mice was based on the type of drug, the highest birth weight was related to the drug Nestonal (TP) and the lowest birth weight was related to the drug Fentanyl (PH). Based on the type of drug, the live birth weight of the mice was the highest weight of the live mice related to Nestonal drug (TP) and the lowest was related to Fentanyl drug (PH).Conclusion: Examining anesthetic drugs to find the safest and best drug is of great clinical and research importance. The study of chromosomal abnormalities is one of the main programs of future studies for the analysis of fetal abnormalities. Manuscript profile

Open Access Article

7 - Feeding ecology and diet of Zagros tooth-carp, Aphhanius vladykovi Coad, 1988 in Choghakhor wetland of Chaharmahal-O-Bakhtiary Province
Fardin Shalui Rasool Zamani Ahmad Mahmoodi Farzaneh Nikookhah Hossein Rahimi Pordanjany Mahin Dokht Jafarei

Background & Aim:  Investigating the diet of fishes is important in order to investigate the ecological role and understand their position in the food web chains.Materials & Methods: In this study, Feeding ecology of Aphanius vladykovi in Choghakhor wetland More

Background & Aim:  Investigating the diet of fishes is important in order to investigate the ecological role and understand their position in the food web chains.Materials & Methods: In this study, Feeding ecology of Aphanius vladykovi in Choghakhor wetland of Chaharmahal-O-Bakhtiary Province was evaluated seasonally. In this reason, the relative index of gastrointestinal (RLG), condition factor (CF) vacuity index (VI) and food items between male and female, two size classes in the hot and cold seasons were investigated.Results: Fish diet was primarily composed of crustaceans (Ostracoda, Ampipoda), mollusks (Gastropoda, Bivalvia), aquatic insect larvae (Ephemeroptera, Diptera) and detritus. There was no significant sex and size-related variation in food items. Diet composition was significantly different between hot and cold seasons (p < 0/05). Furthermore, the average of RLG index was 0.885±0.51that indicated the fish was carnivorous.Conclusion:  In general, in this study, based on the average relative length of the intestine, A. vladykovi is introduced as a carnivorous fish. It is also at an average level in terms of nutrition. The change of season had an effect on the diet of this species, but the studied biological variables such as gender and size were not effective in determining the choice of food items. Manuscript profile

Open Access Article

8 - The effect of a session of intense resistance activity with glutamine supplementation on the relative expression of myogenin and myosin creatine kinase genes in the fast-twitch muscle fibers of adult male Wistar rats.
mansoor mottahedi Tahereh Bagherpoor َArdeshir Zafari nematallah nemati

Background & Aim: Despite the importance of resistance protocol and glutamine on hypertrophy, their effect on myogenic genes expression process is still unknown. Therefore, the aim of the present study was to investigate the effect of an intense resistance session w More

Background & Aim: Despite the importance of resistance protocol and glutamine on hypertrophy, their effect on myogenic genes expression process is still unknown. Therefore, the aim of the present study was to investigate the effect of an intense resistance session with glutamine on Myogenin and Myosin creatine kinase gene expression in male Wistar rats. Materials & Methods: 30 8-week-old male rats with an approximate weight of 220±20 were prepared and divided into three groups, control, intense resistance training, and intense resistance training with glutamine, in a simple random manner. The training groups participated in a resistance session of climbing the ramp with 4 sets, 5 repetitions, 30 seconds of rest between repetitions and 2 minutes of rest between sets. Glutamine was once a day powder dissolved in 100 cc of distilled water at a dose of 5.5 grams per kilogram of body weight every day for 5 days. The Extensor Digitorum long muscle tissue was sent to the relevant laboratory to study the expression of Myogenin and Myosin creatine kinase genes. The relative fold change method was used to check gene expression data at a significance level of 5%. Results: The gene expression results showed that myogenin and myosin creatine kinase gene expression levels increased significantly as a result of a high-intensity resistance training session with glutamine compared to the control group, and this value was more pronounced in the resistance training group (p<0.05). Conclusion: It seems that an intense resistance training session is more effective than glutamine on the increase of myogenic genes. Manuscript profile

Open Access Article

9 - Evaluation of the effect of colostrum in diet on intestinal microbial flora of Japanese quail
Saman Mahdavi Ali Nobakht Reza Mokhtarian asl

Introduction & Aim: Addition of natural nutritional compounds including colostrum in the diet can be useful for quail. The aim of this study was evaluation of the effect of colostrum in diet on intestinal microbial flora of Japanese quail. Materials and Methods: 12 More

Introduction & Aim: Addition of natural nutritional compounds including colostrum in the diet can be useful for quail. The aim of this study was evaluation of the effect of colostrum in diet on intestinal microbial flora of Japanese quail. Materials and Methods: 120 Japanese quails were tested in a completely randomized design using a diet without colostrum, a diet with 2% cow's colostrum and 4% cow's colostrum in 3 treatments and 4 replications and 10 Japanese quails in each replication. After a period of 42 days, two quails were selected from each replicate. Then, a piece of the ileum of each bird was examined to count the population of lactobacillus and coliform. Results: Quails fed with 4% colostrum had the highest total counts of lactobacillus (p< 0.05) and lowest total counts of coliform bacteria among the experimental groups (p>0.05).  Also, the treatment group fed with 2% colostrum also showed an overall increase and decrease in the population of lactobacilli and coliform bacteria compared to the control group (p>0.05).         Conclusion: The use of pasteurized cow colostrum in amounts of 2% and 4% in the diet of Japanese quail increases the total count of lactobacilli and reduces the total population of coliform bacteria, which can be used as a new nutrient to improve the intestinal microbial flora in the diet of farmed poultry.  Manuscript profile

Open Access Article

10 - The effect of two types of exercise (continuous and intermittent) and atrostatin on the expression of PP2Ac and GSK-3β genes in the heart tissue of diabetic rats
Maryam Ebrahimi Habib Asgharpour parvin farzanegi Reza Rezaee Shirazi

Background and Aim: Apart from high blood pressure and coronary artery disease, diabetes can directly affect the structure and function of the heart and lead to a complication called diabetic cardiomyopathy. The purpose of this study is to investigate the effect of two More

Background and Aim: Apart from high blood pressure and coronary artery disease, diabetes can directly affect the structure and function of the heart and lead to a complication called diabetic cardiomyopathy. The purpose of this study is to investigate the effect of two types of training and the drug atrostatin on the expression of PP2Ac and GSK-3β genes in the heart tissue of diabetic model rats.Methods and Materials: 64 male rats, which were randomly divided into 8 groups: including 1) control, 2) diabetic, 3) diabetic + persistent, 4) Diabetic + periodic, 5) atherostatin, 6) continuous + atrostatin, 7) periodic + atrostatin and 8) saline. The rats of the exercise groups performed two types of continuous and intermittent exercise 5 days a week for 8 weeks. Atrostatin was injected intraperitoneally daily at a dose of 20 mg per kg body weight.Results: Induction of diabetes led to a decrease in the expression of the PP2Ac gene and an increase in the expression of the GSK-3β gene in the heart tissue, and continuous exercise and the combination of intermittent and continuous exercise with the drug atrostatin led to an increase in PP2Ac and a decrease in GSK-3β in the heart tissue of diabetic rats compared to the diabetic group.Conclusion: It is possible that regular exercise in combination with atrostatin can prevent the development of diabetes-induced cardiomyopathy by increasing the PP2Ac gene and decreasing the GSK-3β gene in the heart tissue which has a protective effect on the heart of diabetic rats. Manuscript profile

Open Access Article

11 - Investigation of antimicrobial properties of green synthesized CuO nanostuctures using Berberis integerrima aqueous extract on infected surgical wound caused by Staphylococcus aureus
Parvin Eskandari Faeze Naghilonia Reza Shapouri Mahdi Arfaei

Background & Aims: CuO is one of the most important transition metal oxides due to its interesting properties. It is used in various technological applications such as high critical temperature superconductors, gas sensors, photocatalyst, and so on. Staphylococcus a More

Background & Aims: CuO is one of the most important transition metal oxides due to its interesting properties. It is used in various technological applications such as high critical temperature superconductors, gas sensors, photocatalyst, and so on. Staphylococcus aureus bacteria causes a wide range of infectious diseases. In this study, the antimicrobial activity of the synthesized copper oxide nanoparticles in the presence of Berberis integerrima aqueous extract on the infected surgical wound caused by this bacterium was investigated in mice.                             Materials and methods: First, copper oxide nanoparticles were synthesized in the presence of Berberis integerrima aqueous extract and confirmed by XRD, FT-IR, FE-SEM analyses, and the MIC and MBC levels of the nanostructures used were determined by the dilution method in Mueller Hinton Broth culture medium. Also, after creating a skin wound and inoculating the solution of Staphylococcus aureus bacteria with a concentration of (5 × 105 CFU/ml), the concentration of MBC along with Eucerin ointment was checked on the infected wounds.           Results: In general, copper oxide nanoparticles synthesized in the presence of Berberis integerrima aqueous extract has a significant antimicrobial activity to control the growth of Staphylococcus aureus bacteria. Also, the synthesized copper oxide has a favorable antimicrobial and reparative effect on the infected surgical wound caused by this bacterium.                           Conclusion: According to the findings, copper oxide nanoparticles can be effectively and efficiently synthesized by Berberis integerrima aqueous extract in simple and environmentally friendly conditions. It also has a significant antibacterial effect on Staphylococcus aureus bacteria and has a favorable reparative effect compared to Eucerin ointment. Copper oxide nanoparticles can be expected to play an effective role in the treatment of microbial infections.                                  Key words: Berberis integerrima, copper nanoparticles, Eucerin, mice, Staphylococcus aureus. Manuscript profile

Open Access Article

12 - Comparison the effect of melatonin and the selective inhibitor of serotonin reuptake citalopram drug on the ultrastructure of liver tissue and hepatic enzymes in Adult Female three-spotted gourami Fish (Trichogaster trichopterus)
Mohammadreza Pouravaz طاهره ناجی Homayon Hosseinzadeh sahafi

Background & Aims: Most of the various drugs that are used to treat various diseases are metabolized in the liver. The purpose of this study is to investigate the effect of melatonin and selective serotonin reuptake inhibitor drug citalopram on liver tissue ultrastr More

Background & Aims: Most of the various drugs that are used to treat various diseases are metabolized in the liver. The purpose of this study is to investigate the effect of melatonin and selective serotonin reuptake inhibitor drug citalopram on liver tissue ultrastructure and liver enzymes in adult female Trichogaster. Materials & Methods: For this purpose, 120 pieces of Trichogaster with an average weight of 4.23 ± 0.70 g divided into eight groups of 15, which included the control groups, solvent intact, three groups which received citalopram with a dose of 5, 10 and 20 mg/kg and three treatment groups receiving melatonin with doses of 10, 50, and 100 mg/kg. A dose of 0.02 ml of the drug was injected intramuscularly between the dorsal fin and the lateral line every other day for 10 times, over a period of 20 days. Then, the fish were euthanized and dissected. Results: The results showed that melatonin and citalopram had a significant effect on the level of all liver enzymes compared to the control groups (P<0.05). The highest levels of ALT, AST and ALP were measured after the injection of 20 mg/kg citalopram (P<0.05). In fish treated with the highest concentration of melatonin and all concentrations of citalopram, dissociation between hepatocytes and expansion of sinusoids occurred. Fat vacuoles were formed in high concentrations of melatonin and citalopram. Conclusion: Therefore, melatonin at a concentration of 100 mg/kg and citalopram at a concentration higher than 5 mg/kg have a negative effect on the ultrastructure of liver tissue and liver enzymes. Manuscript profile

Open Access Article

13 - Effect of low frequency electrical stimulation on CB2 receptors expression level in the dentate gyrus during perforant path kindling in rats
Parastoo Mardani Javad Mirnajafi zadeh

Background & Aim: Application of Low-frequency stimulation (LFS), is accompanied with a decrease in hippocampal kindled-seizure induced changes rats and prevents propagation of seizure. The purpose of this study is to investigate the role of the endocannabinoids recepto More

Background & Aim: Application of Low-frequency stimulation (LFS), is accompanied with a decrease in hippocampal kindled-seizure induced changes rats and prevents propagation of seizure. The purpose of this study is to investigate the role of the endocannabinoids receptors CB2 in the inhibitory effects of LFS on perforant path kindling- arose seizures. Materials & Methods: Rats Animals were kindled through electrical stimulation of perforant path and recorded responses in the dentate gyrus. Animals included of four groups kindled, Kindled+LFS (KLFS), LFS and control. Semi- rapid kindling procedure was used to induce seizures in animals. In group of KLFS, LFS (0.1 ms pulse duration at 1 Hz, 800 pulses) was applied five min after termination of kindling stimulations. At the end of the experiment, after fixing the animals by intracardiac perfusion method with 4% paraformaldehyde, the cerebral hemispheres were examined by Immunofluorescence. Results: Obtained results showed that LFS application in the LFS-treated group (KLFS) had significantly reduced seizures parameters. However, on basis immunofluorescent findings, no significant change in level of expression CB2 receptors was observed following LFS stimulations in the gyrus dentate of the KLFS group while it increased significantly compared to the kindling group. Conclusion: It is suggested that LFS does not interact with CB2 endocannabinoid receptors but in the pathological conditions of kindling, LFS may have an effect in suppressing seizures by increasing the expression level of CB2 endocannabinoid receptors to the normal expression level, although this increase in the expression level was not different from normal conditions. Manuscript profile

Open Access Article

14 - The effect of the combination of black seed and honey (Dosin) on memory and learning following induction of hypothyroidism in adult male rats
Parisa Poorzal Mokhtar Mokhtari Mehrdad Shariati

Background & Aim: Hypothyroidism causes memory and learning disorders and reduced cognitive function. This study was conducted in order to determine the effect of dosin (Negilla sativa and honey) on learning and memory in adult male rats after induction of hypothyroidis More

Background & Aim: Hypothyroidism causes memory and learning disorders and reduced cognitive function. This study was conducted in order to determine the effect of dosin (Negilla sativa and honey) on learning and memory in adult male rats after induction of hypothyroidism. Materials & Methods: In this experimental study, 60 adult male Wistar rats with an approximate weight of 225-200 g and an age of about 2.5-3 months were divided into six groups of ten. The control group (Ctl) did not receive any drug. Sham group receiving one milliliter of distilled water for 21 days, Experimental group 1 of hypothyroidism (Hypo) who received methimazole in the amount of 25 mg/kg by gavage for 10 days. Experimental group 2 receiving Dosin at the rate of 100 mg/kg for 21 days, experimental groups 3 and 4, in addition to inducing hypothyroidism, received 100 and 200 mg/kg respectively for 21 days by gavage. Memory and learning disorders in animals were evaluated by the Morris water maze test. Results: : In the Morris water maze, the time and distance traveled to reach the platform in the experimental group 1 (Hypo) was higher than the control group (Ctl) on the third and fourth days. The time and the route traveled in experimental groups 3 and 4 on the third day were significantly less than the experimental group 1. Spatial memory results showed that the average time spent in the target quadrant in experimental group 1 decreased significantly compared to the control group. Also, the average time and distance spent in the target quadrant in experimental group 3 showed a significant increase compared to experimental group 1. Conclusion: The results suggest that treatment with dossin extract may ameliorate the deleterious effects of hypothyroidism on learning and memory. Manuscript profile

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