List of Articles شهرزاد نصیری سمنانی

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Isolation Of Bacillus cereus from Foods and Studying the Cytotoxicity Of Them on Vero Cells
  Zahra Deilami Khiabani Shahrzad Nasiri Semnani
  Inroduction and Objective:Bacillus cereusis an important enterotoxigenic food borne pathogen. It contains NHE and HBL complex  enterotoxins. The genes of these complex can be used for characterization of  enterotoxin production in B. cereus. The objective of this study More

  Inroduction and Objective:Bacillus cereusis an important enterotoxigenic food borne pathogen. It contains NHE and HBL complex  enterotoxins. The genes of these complex can be used for characterization of  enterotoxin production in B. cereus. The objective of this study is to detect enterotoxigenic B. cereus and assessing the incidence of it in foods.Material and Methods:220 food samples including; 80 meat products, 20 dairy, 20 rice and 100 salad samples, have been collected in the years 2012-2014. Using biochemical and molecular tests, the B. cereus positive food samples has been detected. B.cereus isolates checked for NHE and HBL complex by specific primers. B. cereus isolates which contain both NHE and HBL have been used for cytotoxic assay on Vero cells.Results: Among 100 B.cereus isolates, 40 contain NHE, 23 contain HBL and 17 isolates contain both complexes. The recent isolates have been used for cytotoxic assay on Vero cells.Conclusion: PCR is a prompt, reliable method for detection of enterotoxigenic bacteria. Enhancing awareness about virulence and prevalence of genes involved in food poisoning would be effective in prevention of food poisoning. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Study Effects Of Hydroalcoholic Extract Of Medicago sativa in the Treatment Of Acetic Acid Induced Gastric Ulcer in Rats
  Mojdeh Samari Mehdi Rahnema Shahrzad Nasiri Semnani Ali Shahnavaz
  Inroduction and Objective:Peptic ulcer, from histologicalpointisknownasmucosalnecrosisfactor. The purpose of this study was to investigate the effect of Alfalfa hydroalcoholicvegetivearial organs extract on gastric ulcer induced by acetic acid in Rat.Material and Method More

  Inroduction and Objective:Peptic ulcer, from histologicalpointisknownasmucosalnecrosisfactor. The purpose of this study was to investigate the effect of Alfalfa hydroalcoholicvegetivearial organs extract on gastric ulcer induced by acetic acid in Rat.Material and Methods: In this experimental study, 60 wistar rats weighting approximately (200-250g) were randomly divided into 4 groups; control, sham(normal salin), experiment 1 (extract of dose 250 mg/kg body weight) and experiment 2 (extract of dose 500 mg/kg body weight). Rats were deprived of food and then underwent surgeryand gastric wasby injecting60% acetic acid after 4,7,10,14 days gavage, the rats' stomach out and then measuring the wound and fixed with 10% formalin, the passage of the tissue section was stained And the number of neutrophils, macrophages and fibroblasts were counted.Results:Results showed that the hydroalcoholic extract of Alfalfacaused a significant increase in gastric ulcer healing in experimental groups 1 and 2 (PConclusion:Alfalfahydroalcoholic vegetive arial organs extract is effective in the treatment of peptic ulcer induced by acetic acid. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Study Effects Of Hydroalcoholic Extract Of Melisa Officinalis in the Treatment Of Acetic Acid Induced Gastric Ulcer in Rats
  تکرخ اشتری توان دشتی مهدی رهنما شهرزاد نصیری سمنانی
  Inroduction and Objective:Peptic ulcer involved mucous extras and good heal under mucous in the wall of stomach. This decease usually is from the most known digest deceases, the plant drugs as compare with the chemical drugs had lower side effects and in contrast of che More

  Inroduction and Objective:Peptic ulcer involved mucous extras and good heal under mucous in the wall of stomach. This decease usually is from the most known digest deceases, the plant drugs as compare with the chemical drugs had lower side effects and in contrast of chemical drugs that maybe had interfere with another compounds of  body or produced a poison in the body, hasn’t interfere effects. Melisa officinalis have biologic effects such as calmness and antioxidant effects. In this research studied the effects of hydroalcoholic nectar of leaf ofMelisa officinalis plant over the peptic ulcer result of acid acetic on the RatMaterial and Methods: In this experimental study prepared hydraulic nectar of melisa officinalis plant. 60heads of female mousse in weight of 250-200 divided accidently to four 15groups, control group, Sham group (normal receiver of Salin) experiment1 group (extract of does 200 mg/kg) experiment2 group (extract of does 400 mg/kg). Mousse suffered form peptic ulcer through injection of acid acetic. After a day the rats treated for 14days through nectar process. Then the sample of stomach in the forth, tenth seventh and fourteen days excited and after measuring the sizes of wounds and fitting with formalin 10% , passage stages,  performed tissue cut and coloring method and finally investigated the histology parameters.Results:the results showed that the nectar of Melisa officinalis result in meaningful increase of improving the wound in the experiment group 1 and 2 as compare with Sham and control groups, in the experiment group 2 improving the wound as compare with the experiment group 1 had meaningful increase. The inflammation factors and fibroblasts showed the meaningful decrease as compare with control and Sham group and showed meaningful reduction in the next days and the experiment group 2 showed meaningful reduction from froth day.Conclusion:Melisa officinalishas high recover ability in treatment of peptic ulcer result of acid acetic Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  4 - Evaluation of Aqueous, Organic Extracts of Leaves and Roots Polygonum aviculare L. on Antibiotic-Resistant Pathogenic Bacteria Invitro and Invivo
  shahrzad nassiri semnani nastaran Ghasempour
  Inroduction & Objective: Drug resistance of bacteria in infections diseases has led to more attention to the use of plants in the treatment of diseases. The aim of this study was to investigate the effects of aqueous, organic extracts of leaves and roots of Polygonu More

  Inroduction & Objective: Drug resistance of bacteria in infections diseases has led to more attention to the use of plants in the treatment of diseases. The aim of this study was to investigate the effects of aqueous, organic extracts of leaves and roots of Polygonum aviculare L. on pathogenic bacteria in animal and laboratory models. Materials and Methods: In this study, after preparing aqueous, ethanolic and estonian extracts of different parts of Polygonum aviculare L. powder, the MIC and MBC of the extracts on bacteria were determined by dilution methods in broth and well diffusion in agar. In this study, an animal model was administered by peritoneal injection of 5×105 CFU/ml of bacteria and 0.5 cc of extracts with MIC concentration, number of spleen bacterial colonies after 7 days by culture on Müllerinton agar and standard counting protocol. Results: The highest levels of MIC and MBC on Staphylococcus aureus related to leafy and ethanolic leaves were 57 and 38, respectively, on Pseudomonas aeruginosa related to stem and leaf ethanol, respectively 31 and 27, on Klebsiella pneumoniae ethanolic extract of root and leaf 57 and 120 mg / ml and on Streptococcus pyogenes the estonian extract of the leaf is 227 mg / ml. In vivo, the leaf extract for Staphylococcus aureus was 7.5 × 106 CFU / ml and the ethanolic extract of the stem for Pseudomonas aeruginosa was 8 × 107 CFU / ml, for Klebsiella pneumoniae and Streptococcus pyogenes leaves and roots was 7.5 × 103 and 6.3 × 107 CFU / ml respectively. Conclusion: Polygonum aviculare L.extracts have antimicrobial effects on the studied bacteria. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  5 - The Effect of Hydro Ethanolic Seeds Extract of Neem(Azadirachta indica) and Bakain (Melia azedarach) to the Number of Trypanosoma evansi Steel in Liver of Mice
  shahrzad nassiri semnani nastaran Ghasempour
  0000-0002-7481-1784
  DOR: 20.1001.1.17359880.1400.14.3.3.4
  Inroduction & Objective: Trypanosoma evansi induced trypanosomiasis causes infection in domestic mammals as well as humans. Treatment of trypanosomiasis has limitations, including resistance to chemical drugs. The aim of this study was to identify active natural pro More

  Inroduction & Objective: Trypanosoma evansi induced trypanosomiasis causes infection in domestic mammals as well as humans. Treatment of trypanosomiasis has limitations, including resistance to chemical drugs. The aim of this study was to identify active natural products and compare the effects of hydroalcoholic extracts of neem seeds and bitter olives against the resistant agent of this disease in the liver organ of mice infected with Trypanosoma evansi.Material and Methods: After collecting and identifying neem and Bakain seeds plants, hydroalcoholic extract was prepared from their seeds by Perkoleh method and after concentrating 100-500 mg / kg of rat weight peritoneally to male rats infected with 3×107 Trypanosoma evansi were injected for 3 days. Routine method was used to determine the number of noises. Serum levels of ALT, AST, ALP were measured. Tissue pathology tests of. Histopathological liver tests were performed after hematoxylin and eosin staining. Statistical evaluation was performed using ANOVA analysis of variance and Prism software and the error limit of p <0.05 was accepted as a significant difference.Results: Neem and Bakain seeds extracts both reduced the number of parasites and compared to the two extracts, the reducing effect of Bakain was greater than that of Neem. Congestion changes, fat degeneration, vacuolar degeneration, Necrosis and cell infiltration in the liver nephritis was higher of the control groups than the treated groups.Conclusion: The use of neem and Bakain seeds hydroalcoholic extracts of in a certain amount of herbal medicine is effective in trypanosomosis (soda). Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  6 - Evaluation of the Antimicrobial Effect of Lemon verbena leaves Extracts on Streptococcus pyogenes, Staphylococcus epidermidis, Klebsiella pneumoniae and Brucella melitensis in vitro and Animal Model Study
  shahrzad nasiri semnani nastaran ghassempoor
  Inroduction & Objective:Streptococcus pyogenes and Staphylococcus epidermidis Klebsiella pnenmonia and Brucella melitensis have high resistance to antibiotics treatments. The aim of this study, was to investigate the antimicrobial effect of Lemon verbena leaves extr More

  Inroduction & Objective:Streptococcus pyogenes and Staphylococcus epidermidis Klebsiella pnenmonia and Brucella melitensis have high resistance to antibiotics treatments. The aim of this study, was to investigate the antimicrobial effect of Lemon verbena leaves extracts on Streptococcus pyogenes, Staphylococcus epidermidis, Klebsiella pneumoniae and Brucella melitensis in vitro and animal model study. Material and Method:In this studies after prepagation, of extracts Lemon leaves MIC and MBC and  diameter of inhibition zone was determined by well diffusion agar method, the extracts on bacteria determined by broth dilution method. In animal model study, 5×105 CFU/ml bacteria were injected intraperitioneally in to mice and the counts of bacterias colonies was countected according to the standard protocol. Results: The results of macrodilution method showed that the MIC of ethanolic extract of the leaves of the lemon verbena in the range of 1:32 dilution (27.81 mg/ml) to 1:8 (111.25 mg/ml) and MBC ethanolic extract of the leaves of the lemon verbena  in the range 1:16 (55.62 mg/ml) to 1:4 (222.5 mg/ml). MIC of acetonic extract of the leaves of the lemon verbena in the range of 1:32 dilution (27.81 mg/ml) to 1:8 (111.25 mg/ml) and MBC of acetonic extract of the leaves of the lemon verbena  in the range 1:16 (48.18 mg/ml) to 1:4 (192.75 mg/ml). Also MIC of aqueous extract was in the range of 1:8 (103.75 mg/ml) to 1:4 (207.5 mg/ml) and MBC of this extract was in 1:4 dilutions (207.5 mg ml) in all studied bacteria. In animal model, were seen a significant reduction between the treated groups with control group. Ethanolic and acetonic extract Lemon verbena more effective in an animal model. Conclusion: The results of this study showed that Lemon verbena extracts has antimicrobial effect on studied bacteria Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  7 - Investing effect of Malva , Thymus , Viola and Pinus, Extracts on Candida Glabrata in the In vitro and Animal model.
  Seyedeh maryam Moosavinejad shahrzad Nasiri Semnani Rasol Shikri Reza Shapouri
  Inroduction & Objective:The skin infected wound and wounds caused by burn are very epidemic in society, and these kind wounds rate treatment in effective to alleviate the patint, applying the herbal drugs with less complications to treat the wounds. Considering this More

  Inroduction & Objective:The skin infected wound and wounds caused by burn are very epidemic in society, and these kind wounds rate treatment in effective to alleviate the patint, applying the herbal drugs with less complications to treat the wounds. Considering this significance , surveying Investing effect of Malva , Thymus , Viola and Pinus, Extracts on Candida Glabrata in the In vitro and Animal model. Material and Method:In this study, hydroalcholic extracts of Malva , Thymus , Viola and Pinus were prepared in the laboratory. Then, the MIC and MLC of the extract were determined by dilution method in the broth. In the study of the animal model, the bacteria were first inoculated with a concentration of (5×106CFU/ML) to the wound site on Syrian mice. After 24 hours, an ointment prepared with MLC concentration was prepared from extracts was used to treat burns and infections withCandida Glabrata. In the animal model, the extracts of have antimicrobial activity against growth of Candida Glabrata. Results: In this study, The MIC and MLC of the extracts model, the microbia were first inoculated with a concentration of (5×106CFU/ML) to the wound site on Syrian mice. After 24 hours, an ointment prepared with MBC concentration was prepared from extracts of the nettle, watercress and burdock root for 1 g of silver sulfadiazine and was used to treat burns and infections with Candida Glabrata. In the animal model, the extracts antimicrobial activity against growth of Candida Glabrata.In this case, the extract of Pinus and Viola have growth inhibitory Candida Glabrata activity stronger than Malva , Thymus extract. Conclusion: According to the findings of this study, it can be concluded that the extract of these 4 plants on an animal model has antimicrobial and healing effects on Candida Glabrata. It can be used as an anti-inflammatory agent orointment microbia to be raised. Manuscript profile