Evaluation of Aqueous, Organic Extracts of Leaves and Roots Polygonum aviculare L. on Antibiotic-Resistant Pathogenic Bacteria Invitro and Invivo
Subject Areas :
shahrzad
nassiri semnani
1
(Assistant Professor in Department of Microbiology, Factualty of Bacic Sciences -Engineering, Zanjan Branch, Islamic Azad university, zanjan.Iran.)
nastaran
Ghasempour
2
(PhD. student General Veterianary, Factualty of Veterianary Razi University, Kermanshah. Iran)
Keywords:
Abstract :
Inroduction & Objective: Drug resistance of bacteria in infections diseases has led to more attention to the use of plants in the treatment of diseases. The aim of this study was to investigate the effects of aqueous, organic extracts of leaves and roots of Polygonum aviculare L. on pathogenic bacteria in animal and laboratory models. Materials and Methods: In this study, after preparing aqueous, ethanolic and estonian extracts of different parts of Polygonum aviculare L. powder, the MIC and MBC of the extracts on bacteria were determined by dilution methods in broth and well diffusion in agar. In this study, an animal model was administered by peritoneal injection of 5×105 CFU/ml of bacteria and 0.5 cc of extracts with MIC concentration, number of spleen bacterial colonies after 7 days by culture on Müllerinton agar and standard counting protocol. Results: The highest levels of MIC and MBC on Staphylococcus aureus related to leafy and ethanolic leaves were 57 and 38, respectively, on Pseudomonas aeruginosa related to stem and leaf ethanol, respectively 31 and 27, on Klebsiella pneumoniae ethanolic extract of root and leaf 57 and 120 mg / ml and on Streptococcus pyogenes the estonian extract of the leaf is 227 mg / ml. In vivo, the leaf extract for Staphylococcus aureus was 7.5 × 106 CFU / ml and the ethanolic extract of the stem for Pseudomonas aeruginosa was 8 × 107 CFU / ml, for Klebsiella pneumoniae and Streptococcus pyogenes leaves and roots was 7.5 × 103 and 6.3 × 107 CFU / ml respectively. Conclusion: Polygonum aviculare L.extracts have antimicrobial effects on the studied bacteria.
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