Evaluation of UV-A Irradiation and Silver Nanoparticle Treatment on some of Growth, Physiological and Biochemical Characteristics of Dill (Anethum graveolens L.)
Subject Areas : Agriculture and climate
Mahboubeh Sadat Hoseini
1
,
Mehrnaz Mahmoudi-Zarandi
2
*
1 - Department of Biology, Ke.C., Islamic Azad University, Kerman, Iran
2 - Department of Biology, Ke.C., Islamic Azad University, Kerman, Iran
Keywords: Ultraviolet radiation, Antioxidant enzymes, Growth parameters, Protein, Stress, Photosynthetic pigments,
Abstract :
The global security of agricultural products has been threatened due to climate changes and the increase in ultraviolet radiation. Considering the effectiveness of nanoparticles in improving plant resistance to stress, nanotechnology can be used to reduce the effects of stress. This research aims to investigate the UV-A stress on the dill and evaluate the effect of silver nanoparticles in reducing the damage of this stress on the plant. First, pot cultivation of dill seeds was done, when the plant grew to 8 cm, UV-A stress was applied at three levels (0, 30 minutes, and 60 minutes), at the same time, the samples were treated with silver nanoparticles at three levels(0, 50 and 100 mg/L).The growth parameters (fresh and dry weight of stem and root, length of stem and root), physiological parameters (photosynthetic pigments), biochemical parameters (catalase activity, ascorbate peroxidase activity, protein content).The results showed that ultraviolet radiation causes a significant decrease in the growth parameters and photosynthetic pigments of chlorophyll a, b, and total Chl., however, the amount of carotenoids decreased less compared to chlorophylls, so the ratio of carotenoids to total chlorophyll increased. Ultraviolet radiation significantly reduced catalase enzyme activity and protein content, while significantly increasing ascorbate peroxidase enzyme activity. Treatment of plants with silver nanoparticles mitigated the damaging effects of UV radiation, and a concentration of 100 mg/L was identified as the optimal concentration of silver nanoparticles for improving both growth and biochemical parameters of plants exposed to 30 minutes of UV-A radiation. Therefore, application of silver nanoparticles at an appropriate concentration is recommended to enhance plant growth and performance under the detrimental effects of ultraviolet radiation
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