Investigating the amount of berberine alkaloid extracted from the root of barberry (Berberis vulgaris) native to Iran by HPLC method
Subject Areas : Journal of Quality and Durability of Agricultural Products and Food Stuffs
Elham Shahriari Rad
1
(Ph. D. student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
Shahram Shoeibi
2
(Professor, Food and Drug Laboratory Research center, Food and Drug Administration, Ministry of Health and Medical education, Tehran, Iran.)
Shahin Bonakdar
3
(National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran)
akram eidi
4
(Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
Dariush Minai-Tehrani
5
(Associate professor, Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.)
Keywords: Alkaloids, HPLC, Berberine, B. vulgaris, Barberry,
Abstract :
Today, traditional and complementary medicine has a special place in the treating of many disorders and diseases. Valuable medicinal plants grow in Iran due to the special climate diversity. The barberry plant (Berberis vulgaris) is one of the most important and prominent plant species and has many therapeutic properties. Berberine, as the most important barberry alkaloid in the root, has a suitable concentration for extraction and utilization and introducing it as a natural substance with pharmacological properties is the aim of this research. The roots of the barberry plant were collected from Tehran city and ground after drying at ambient temperature and away from sunlight. Methanol was used to extract the berberine present in the root of this plant. After filtering, the methanolic extract was used to identify berberine using an HPLC device with a DAD detector. The resulting chromatogram shows the proper separation of barberry root extract and berberine peak peaks compared to the berberine standard, which was clearly separated and identified. The amount of berberine in barberry root was determined according to the calibration curve of 1.44%. Based on the obtained results and a review of the therapeutic and medicinal properties of native Iranian barberry, it is important to determine the amount of the effective substance in the context of its use in the prevention and treatment of various disorders and diseases.
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