Identification of resistant genes against blast in genotypes cultivated in north of Iran using molecular markers
Maryam Farifteh 1 , Bahar Morid 2 , Hamidreza Zamanizadeh 3
1 - Plant Protection Department, Agriculture Faculty, Islamic Azad University Science and Research Branch, Tehran, Iran
2 - Plant Protection Department, Agriculture Faculty, Islamic Azad University Takestan Branch, Takestan, Qazvin, Iran
3 - Plant Protection Department, Agriculture Faculty, Islamic Azad University Science and Research Branch, Tehran, Iran
Keywords: Rice Blast Disease, Resistance gene, Pia, Pib, Pi5 ,
Abstract :
Blast disease caused by Magnaporthe grisea is a significant threat to rice worldwide, leading to substantial damage and yield losses. The most effective method for controlling this disease is through the use of resistant rice cultivars that carry specific resistance genes. In this study, three primers (Yca7, Nsb, and JJ718) were used associated with the Pia, Pib, and Pi5 resistance genes, respectively, to identify blast-resistant rice cultivars and lines. At first, DNA was extracted from the leaves of 10-day-old seedlings of the entered rice cultivars and lines using the CTAB method. Subsequently, polymerase chain reaction (PCR) and electrophoresis were conducted to amplify and analyze the resulting DNA bands with the respective primers. Our findings indicated that Qaim and Gilane cultivars carry the Pia resistance gene, making them probable candidates for rice blast disease resistance. Similarly, all tested cultivars (excluding Sahel) possess the Pib resistance gene, suggesting their resistance to blast disease. Furthermore, except for Sepidrood and Sahel cultivars, all cultivars tested positive for the Pi5 resistance gene, indicating their ability to resist blast disease. Based on these results, Qaim and Gilane cultivars exhibit resistance to the disease as they carry all three resistance genes, while Sahel cultivar lacks all three resistance genes, making it the most susceptible cultivar in our study.
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