Evaluation of the antagonistic ability of two isolates of Trichoderma harzianum against Tobacco root-knot nematode (Meloidogyne incognita)
Subject Areas : Plant Pathology
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Keywords: Biological control, antagonistic, Nicotiana tabacum, Trichoderma harzianum BI, T. harzianum ,
Abstract :
Root knot nematodes (Meloidogyne sp.) are one of the most important plant parasitic nematodes in the world. In recent years, the biological control of root knot producing nematodes is considered a priority, with the aim of reducing the dangerous effects of chemical toxins, including threats to human health and environmental pollution. Antagonists are one of the effective factors in biological control. In order to biologically control tobacco root knot nematode (Meloidogyne incognita), the effect of two isolates of Trichoderma harzianum were investigated in laboratory conditions in a Completely Randomized Design (CRD) project in 4 replications. In laboratory studies, the effect of the culture extract of two isolates of Trichoderma, including Trichoderma harzianum BI and Trichoderma harzianum Iran 2375c with a concentration of 106 spores/mL, on the mortality of the second instar larvae and hatching the eggs of M. incognita in tobacco were evaluated at 48 and 72 hours. The average comparison showed that the most effective treatment was the T. harzianum BI isolate, after 14 days, caused 86.7 % decrease in egg hatching rate and after 72 hours, caused 69.5 % on the mortality of second instar larvae of M. incognita. In general, the results obtained from this research showed that the culture extracts of both T. harzianum isolates have good antagonistic ability against the M. incognita.
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