Determine the antibiotic resistance patterns and tsst-1 gene frequency in staphylococcus auresu strains isolated from patients of Imam Khomeini hospital in Ahvaz
Subject Areas : microbiology
ziba shanaki baversad
1
(1. MSc, Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord Branch, Iran)
مریم ریسی
2
(گروه میکروب شناسی،دانشگاه آزاد اسلامی، واحد شهرکرد، گروه میکروب شناس)
Marziyeh Soleymanian
3
()
Keywords: Antibiotic resistance, Staphylococcus aureus, Toxic shock syndrome toxin -1 gene,
Abstract :
isolates of Staphylococcus aureus from different samples to determine antibiotic resistance and Staphylococcus aureus is a major cause of hospital acquired infections and community. Toxic shock syndrome toxin -1 gene secreted by the bacteria from the categories are important virulence factors and is component super antigens toxins pyrogenic (PTSAgs). The purpose of this study is determine the antibiotic resistance patterns and tsst-1 gene frequency in Staphylococcus aureus strains isolated from patients of Imam Khomeini hospital in Ahvaz. In this study, 133 clinical frequency tsst-1 gene were studied. After genomic DNA extraction using DNA extraction kit was performed the definitive diagnosis of bacteria, Then the gene tsst-1 frequency done in the presence of specific primers and antibiotic resistance was determined by agar disk diffusion method. After PCR amplification and detection of the bacterium, Of 133 isolates sequence tsst-1 gene was observed in 6 strains. In antibiogram test the greatest resistance to cefazolin (3/83%) and the lowest resistance to nitrofurantoin and vancomycin (0%) was observed. Due to the increasing prevalence of resistance to antibiotics of clinical importance tsst-1 gene timely identification and implementation of appropriate therapeutic strategies for controlling infection seems necessary.
1. Stuart Walker T. 1998. Microbiology walkers. Michigan: W.B. Saunders Company..
2. Reisi M., Tajbakhsh E. and Momtaz H. 2014. Isolation and identification of antibiotic resistance genes in Staphylococcus aureus isolates from respiratory system infections in Shahrekord, Iran. Biol J Micro. 10: 97-106.
3. El-Ghodban A., Ghenghesh K.S., M.rialigeti K., Esahli H. and Tawil A.2006. PCR detection of toxic shock syndrome toxin of Staphylococcus aureus from Tripoli, Libya. J. Med. Microbiol .55(2):179-82.
4. Tihoo M., Mobin H., Mozafari N.A., Moadab R., Sedigh bayan K.H. and Mones rast S.H. 2011. frequently distribution toxic shock syndrome-1 (TSST-1) in S. aureus strains isolated from hospitals in Tabriz Shohada. Lab .Sci .J. 1: 39-44.
5. Nakagava S., Kushiya K., Uchiyama T. and Yamamoto T.2005. Specific inhibitory action of anisodamine against a staphylococcal superantigenic toxin, toxic shock syndrome toxin 1(TSST-1), leading to down-regulation of cytokine production and blocking of TSST-1 toxicity in mice. Clin. Diagn. Lab. Immunol. 12: 399-408.
6. Bacha EA., Sheridan RL, Donohue GA. and Tompkins RG. 1994. Staphylococcal toxic shock syndrome in pediatric burn unit.Burns. 20: 499-502.
7. Clinical and Laboratory Standards Institute (CLSI). 2006.Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved standard-Ninth Edition (M2-A9).Clinical and Laboratory Standards Institute, Wayne, PA 2006.
8. Erajian G.H., Boromand M.A., Rashedi marandi F., Rahbar M., Shahcheraghi F. and Sharifi M. 2012. Standard performance tests to determine the antimicrobial susceptibility disk diffusion method. Tehran: Voi. Pub. Cen.
9. Farahmand A, Ahmadi S, Dastmalchi Saei M, Anassori H. Identification of toxic shock syndrome toxin-1 (TSST-1) gene in Staphylococcus aureus isolated from bovine mastitis milk. Archives of Razi Institute 2013; 68:17-22.
10. Somily AM, Babay HA. Superiority of Dzonetesting method over standard method to detectRnducible resistance in gram positive bacteria: a Prospective surveillance from a teaching hospital in Saudi Arabia. Int. J. Health .Sci. 2: 8-16.
11. Byrne-Bailey K.G., Gaze W.H., Kay P., Boxall A.B.A., HawkeyPM. and Wellington E.M.H. 2009. Prevalence of Sulfonamide Resistance Genes in Bacterial Isolates from Manured Agricultural Soils and Pig Slurry in the United Kingdom.Antimicrob Agents Chemother. 53(2): 696-702.
12. Barber M. and Rozwadowska- Dowzenko M. 1984. Infection by penicillin- resistant Staphylococci. Lancet. 2: 6530: 641-644.
13. Kirby WM. 1994. Extraction of a highly potent penicillin inactivator from penicillin resistant Staphylococci. Science. 99 (2579): 452- 453.
14. Ramdani- Bouguessa N, Bes M, Meugnier H, Forey F, Reverdy ME, Lina G, et al. Detection of methicillin- resistant Staphylococcus aureus strains resistant to multiple antibiotics and carrying the panton- valentine leukocidin genes in an Algiers hospital. Antimicrob Agents Chemother 2006; 50 (3): 1083- 1085.
15. Rashidiyan M, Taherpour A, Godarzi S. The frequency of nasal carriers of Staphylococcus aureus strains isolated from clinical staff in Besat hospital and resistance to antibiotics of strains isolated. Kurdistan Univ Med J 2002; 6 (21): 2- 6.
16. Ghassemian R, Najafi N, Shojaeifar A. The prevalence of Staphylococcus aureus nasal carriers and pattern Antibiotic resistance in the Employee Training Center –Razi GhaemShahr City in fall 2004. MazandaranUniv Med J 2004; 14 (44): 79- 86.
17. Gohnson W, Tyler M, Ewan S, Ashton E, Pollard F, Rozee KR. Detection of Genes for Entrotoxins, Exfoliative Toxins and Toxin Shok Syndrome Toxin 1 in Staphylococcus aureus by the Polymerase Chain Reaction.J of Clinica Microbiology 1991; 29(3): 426-430.
18. Chiang YC, Liao WW, Fan CM, Pai WY, Chiou CS, Tsen HY. PCR detection Staphylococcal ektrotoxins(SEs) N, O, P, Q, R, U and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases hn Taiwan. Int Food Micobiol 2008; 121: 66-73.
19. Mehrotra M, Wang G, Johnson WM. Multiplex PCR for Detection of genes for Staphylococcus aureusentrotoxin, exfoliative toxins, toxic shock syndrome toxin 1, and meticillin resistance. J Clin Microbiol 2000; 38: 1032-1038.
20. Deurenberg H, Nieuwenhuis F, Driessen C, London N, Frank R, Ellen E. The prevalence of the Staphylococcus aureus tst gene among community and hospital acquired strains and isolates from Wegener-s Granulomatosis patients. FEMS. Micobiology. Letters. 1: 185-189.
21. Islam M.J., Uddin M.S., Nasrin M.S., Nazir K.H.M., Rahman M.T. and Alam MM. Prevalence of Toxic Shock Syndrome Toxin-1 producing coagulase positive Staphylicoccus aureus in human and their characterization. Bangl J Vet Med 2007; 5(1&2): 115-119.
22. Parsonnet J., Goering R., Hansmann M., Jones M., Ohtagaki K., Davis C. and Tutsuka K. 2008. Prevalence of Toxic Shock Syndrome Toxine-1 (TSST-1) Producing strains of Staphylococcus aureus and Antibody to TSST-I among Healthy Japanese Women. J. Clin. Microbiol. 48: 2731- 2738.
23. Kaufmann S.H.E. and Steward M.W. 2005.Topley and Wilson’s Microbiology and Microbial Infections.10th edition, Hodder Amold Ltd, London pp.577-632.
24. Becker K Roth R. and Peters G. 1998. Rapid and specific detection of toxigenic Staphilococcus aureus: use of two Multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal entrotoxin genes, exfoliative toxin genes and toxic shock syndrome toxin 1 gene. J. Clin. Microbiol. 36: 2548-2553.
25. Bacha E.A., Sheridan R.L., Donohue G.A. and Tompkins R.G. 1994. Staphylococcal toxic shock syndrome in pediatric burn unit. Burns: 20: 499-502.
26. Childs C., Edwards Jones V., Heathcote D.M., Dawson M. and Davenport P.J. 1994.Patterns of Staphylococcus aureus colonization, toxin production, immunity and illness in burned children.Burns 20:514-521.
27. Quan L., Morita R. and Kawakami S. 2010. Toxic shock syndrome toxin. 1.Japan. child. Burns 36: 716-721.
28. White M.C., Thornton K. and Young A.E, 2005. Early diagnosis and treatment of toxic shock syndrome in paediatric burns. Burns. 20: 193-197.
29. Javadi niya S.H., Asqarian pour R., Noorbakhsh S., Soboti B., Shokrollahi M. and Tabatabaeei A. 2014. Comparison Level Toxin TSST-1 in children with fever and without fever burn wounds. Tehran. Med. Univ. J. 72: 113-120.